AtypicalWesternt...
实验概要Peprotech provides a typical Western-transfer protocol (e.g. for the detection of protein X).实验原理Western Transfer, also known as Western Blotting, is a rapid immunoblotting technique for identifying the presence of a particular protein in a complex mixture of proteins such as cell lysates or sera. The technique exploits both the efficiency of SDS-PAGE to separate a mixture of proteins into distinct prot......阅读全文
RPL22基因突变因子与药物介绍
核糖体是催化蛋白质合成的细胞器，由一个小的40s亚基和一个大的60s亚基组成。这些亚基由4种RNA和大约80种结构上不同的蛋白质组成。这个基因编码一种细胞质核糖体蛋白，它是60S亚单位的一个组成部分该蛋白属于核糖体蛋白L22E家族其起始的蛋氨酸残基在翻译后被去除。该蛋白能与EBERs（EBERs）1
可靠的CCCadvanced－FN1无异源耗材支持人间充质干细...（四）
Figure 5: hMSC proliferation rate during long-term expansion in different animal-component-free culture systemshMSC expansion on the FN1 motifs surface
毫米波隔离器最新技术进展（一）
Recent Advancements in mmWave Isolator TechnologyMarch 13, 2019 David W. Porterfield, Jr., Micro Harmonics Corp., Fincastle, Va. No CommentsIsolators
细胞周期的流式细胞伩检测实验方法（PI，Brdu)1
ANALYSIS OF CELL CYCLE Miriam Capri and Daniela Barbieri Dept. Biomedical Sciences, Sect. General Pathology,Via Campi, 287, University of Modena, 4110
冻融过程对青藏高原高寒草甸温室气体交换过程影响
温室气体浓度升高引起的全球气候变暖是当今人类社会可持续发展面临的重大挑战。青藏高原既是全球变化的敏感区域，也是世界上低纬度冻土的集中分布区。春季土壤冻融交替是高原地区常见的自然现象，冻融交替不仅能够改变土壤的水热条件、理化性质，而且会显著影响温室气体地气界面交换过程。图：青藏高原典型高寒草甸甲烷
Structure－of－Mitochondria
The cytoplasm of nearly all eukaryotic cells contain mitochondria, although there is at least one exception, the protist Chaos (Pelomyxa) carolinensis
实验室气体汇流排系统的安装方法
是将液态气体经低温泵汽化器通过该系统对气瓶充装或是将一定数量的气瓶同时连接在一起，统一汇流减压，然后以一定的压力输出到每个用气点。该系统结构合理，节约能源，减轻劳动强度，提高工效，保持工作环境的整洁安全。可根据不同的用量和厂房面积，进行设计以保证zui佳的使用效果。1. 开启：应缓慢开启减压器前的截
ELISA:－Sandwich
实验概要The following protocol provides a method of sandwich ELISA by Peprotech.主要试剂Tween-20 (Sigma Cat. # P-7949); BSA (Sigma Cat. # A-7030); ABTS Liqu
湖泊中被忽略的无机还原态磷生态效应研究获进展
全面阐明湖泊磷循环过程并揭示其对生态系统影响一直是环境科学领域研究热点。由于传统认知和分析方法不足, 绝大多数研究均以正五价的磷酸盐(P+V)为基础, 忽略了磷化氢(P-III)、次亚磷酸盐(P+I)和亚磷酸盐(P+III)等无机还原态磷(简写“IRP”), 致使当前对湖泊磷生物地球化学循环过程认识
梅特勒托利多XP205DR电子天平
XP205DR电子天平产品详细说明 功能和特点SmartGrid: 不寻常的称量特性,革新性的网格秤盘,将室内空气气流干扰降至最低.实现最快的称量结果显著降低天平的稳定时间.SmartSens: 实现无需用手接触的天平操作. 操作人员可以全神贯注的进行样品的安全处理, 避免溢出, 并将污染的风险最
Can－Pipetting－Damage－Your－Health
The Art of Ergonomics: Making the Work Fit the WorkerIn a working environment, use of ergonomic principles reduces levels of physical and mental s
Protein－arginine－methylation
Typical modification sites: RGG box or RXR sequence motifs R-arginine, G-glycine,X-any aminoacid.Enzymes catalysing protein arginine methylation: PRMT
石墨烯在太赫兹频段实现的无线片上网络（WiNoC）（一）
On the Nanocommunications at THz Band in Graphene-Enabled Wireless Network-on-ChipQuoc-Tuan Vien,1 Michael Opoku Agyeman,2 Tuan Anh Le,1 and TerrenceM
重组DNA的分离、克隆与测序实验手册8
B. Midiprep double-stranded DNA isolationA midi-prep double-stranded DNA isolation has been developed to generate a sufficient amount of template DNA
Mitochondria－Structure－and－Function－－Theoretical－Details
Mitochondria Structure and Function - Theoretical DetailsIn an attempt to be concise and understandable, introductory level courses and textbooks freq
EMC－Antenna－Parameters－and－Their－Relationships（三）
wherePd = radiated power density at distance r from the antenna, W/m2Pt = power input to the antenna, WGt = numerical gain of the antennar
碳水化合物分析
Carbohydrate Assay (Hancock Laboratory) (Accessible only by IE)This protocol is used to determine the relative amounts of LPS CHO present in a given s
线粒体的结构与功能
In an attempt to be concise and understandable, introductory level courses and textbooks frequently present concepts that are technically correc
a－pipeline－for－the－identification－of－intact－Nglycopeptides（五）
Alignment between MS/MS and MS3 identifications. Glycans were identified after analyzing the HCD/CID-MS/MS spectrum pairs, but the sequences of pe
用Fluidigm－digital－array芯片研究癌细胞
Technology Review网站在2009年9月30日发表了题为《Analyzing Cancer Cells to Choose Treatments—— Microfluidics chips allow scientists to study circulating cancer
Bacteria－Growth－and－Culture－Bacteria－Growth－and－Culture
Flagella and motilitymonotrichous flagella - the bacterial cell has a single flagellaperitrichous flagella - the bacterial cell has several flagella w
Bacteria,－cont.－Growth－and－Reproduction
Flagella and motilitymonotrichous flagella - the bacterial cell has a single flagellaperitrichous flagella - the bacterial cell has several flagella w
In－VitroCulture－of－Chicken－Heart
The chicken is a classic organism used to illustrate the principles of basic embryology. One of the developmental systems which has been examined in g
Dynabeads®－Streptavidin－Trial－Kit（二）
3. General Immobilization Protocol Wash the Dynabeads® before use. a) Add the biotinylated molecule to the washed Dyn
Polymerase－Chain－Reaction－(PCR)－cont.
Polymerase Chain Reaction (PCR) cont.Choice of Polymerases for PCROne of the important advances which allowed development of PCR was the availability
使用CCCadvanced™FN1无异源耗材培养人多能干细胞（一）
Ready-to-use Eppendorf CCCadvanced™ FN1 Motifs Surface for Xeno-Free Expansionof Human Pluripotent Stem CellsAurélie Tacheny¹, Silvia Tejerina¹, Wiâme
An－Ultrafast－method－of－DNA－extraction－from－Neurospora
We have found that the DNA extraction procedure of Metzenberg and Baitch (Neurospora Newsl. 28:20)/Stevens and Metzenberg (Neurospora Newsl. 29:27) wh
Basics－of－Electrochemical－Impedance－Spectroscopy（五）
From this equation the exchange current density can be calculated when Rct is known.DiffusionDiffusion also can create an impedance called a Warburg
Chemical－Induction－of－Apoptosis
Chemical Induction of Apoptosis - 1 May 2001p53, p21WAF1, Myc, Bcl-2, Bax, Bcl-x and bak are among the proteins involved in the regulation of apoptosi
Cr－Release－Cytotoxicity－assay
DescriptionCytotoxic activities of mNK cells are examined in 51Cr release assay from target cells ProcedureEffector cells (mNK cells) are seeded into