Wholemountfluores...
实验概要The method provides a protocol for whole mount fluorescent immunohistochemistry. The advantage of using fluorescence to stain whole mount sections is that confocal microscopy can be used to section through the larger embryo or tissue sample without having to manually section onto slides. This gives a clearer idea of where the target protein of interest is expressed within the t......阅读全文
骨组织βgal免疫染色实验技术方法
ß-gal Staining:Reagents:0.1M Phosphate Buffer (pH 7.3):0.1M Sodium Phosphate monobasic115ml0.1M sodium phosphate dibasic385mlTotal Volume500 mlFix sol
显微镜接口知识
什么叫C/CS接口?单词“C”的来历是“cinema”(电影)的打头字母,C接口的起源是摄影机镜头。目前,C接口是由 从16毫米摄影机、电视摄像机、三目显微镜的标准接口。C接口镜头提供一个公口的螺纹与相机的母口配合。螺纹的直径是1英寸(25毫米),每英寸32圈螺纹,根据“ANSI B1.1”标准设计
Early-development-of-primary-motor-neurons-and-somites-in-Zebrafish-Embryos
Background:Zebrafish,or the teleost fish Danio rerio,is a rapidly developing organism that is apopular species for studying vertebrate development. Cl
Specimen-Preparation-for-Scanning-Electron-Microscopy
Specimen Preparation for Scanning Electron MicroscopyWe recommend consultation with one of the lab directors before preparing specimens. The methods p
AWorks编程:Shell交互界面(三)
2.4.2 mount命令描述挂载存储设备到指定的文件系统。语法结构mount <mount point> <device name> <FS type> 。取值说明<mount point> 为挂载点的名称,是必填参数,用户可自行取值。<device name> 是设备名称,必填参数。<FS ty
Phase-Contrast-Microscopy
Phase Contrast MicroscopyPrincipleMost of the detail of living cells is undetectable in bright field microscopy because there is too little contrast b
制作光学显微镜切片样本时所需用品简介!
载玻片之品质依不同厂商而有所差异,品质较差者略带有淡蓝色。载玻片又分有磨砂与光滑两种,前者仅在玻片一端带有磨砂,方便拿取,但若要贴上标籤,以光滑玻片较易黏贴。 载玻片的标準尺寸均为75mm(长)×25mm(宽),此规格与染色瓶、样本盒等相互配合,也有特殊规格之载玻片或可以买市售之玻
E.-Immunohistochemi...
实验概要We provide a guideline procedure and tips for staining of paraffin embedded sections, including antigen retrieval, chromogenic detection and flu
用高内涵成像完成3D微组织球三维体积与分区分析的方法
高内涵—3D微组织球三维体积与分区分析 三维多细胞类球体(肿瘤球、微球、类器官)可以帮助我们在临床前药物筛选阶段更好地预测多种候选药物的潜在作用。但是,相较于二维单层培养细胞,采用三维培养细胞模型系统进行检测分析则更具挑战性。 一起来看看珀金埃尔默是如何分析3D微组织球三维体积与分区的吧! 3D微组
用高内涵成像完成3D微组织球三维体积与分区分析的方法
高内涵—3D微组织球三维体积与分区分析 三维多细胞类球体(肿瘤球、微球、类器官)可以帮助我们在临床前药物筛选阶段更好地预测多种候选药物的潜在作用。但是,相较于二维单层培养细胞,采用三维培养细胞模型系统进行检测分析则更具挑战性。 一起来看看珀金埃尔默是如何分析3D微组织球三维体积与分区的吧! 3D微组
粘度计NDJ1-英文说明书
Operation ManualForNDJ-1 Rotary ViscometerMADE IN CHINAThank you for using NDJ-1 Rotary Viscometer. NDJ-1 is easy to use, in order to install and op
用高内涵成像完成3D微组织球三维体积与分区分析的方法
高内涵—3D微组织球三维体积与分区分析 三维多细胞类球体(肿瘤球、微球、类器官)可以帮助我们在临床前药物筛选阶段更好地预测多种候选药物的潜在作用。但是,相较于二维单层培养细胞,采用三维培养细胞模型系统进行检测分析则更具挑战性。 一起来看看珀金埃尔默是如何分析3D微组织球三维体积与分区的吧! 3D微组
红外观察镜的相机适配器运用解析
红外观察镜是一款高性能的图像转换观察镜。其通过将被观测物体所反射或发射的光聚焦到摄像管里而进行观察,光谱响应范围覆盖270-2000 nm。它体积小,重量轻,结构紧凑,手持式方便自由操作,顶端内螺纹规格为1/4-20,内置一节1.5V“AAA”型号电池,也可外接3V的电源。通过持续按住
用DAPI进行细胞染色
用DAPI进行细胞染色DAPI的配置Stock solution of 1mg/ml in ddH2O; working solution of 0.25mg/ml to 50mg/ml in ddH2O or PGM. 1.Place sample on slide. 2.Add a few dr
Differential-Interference-Contrast
Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)PrincipleDifferential interference microscopy requires several optical
全基因组的比较基因组杂交技术介绍
Whole-Genome and Custom Fine-Tiling Array CGHComparative Genomic Hybridization (CGH) measures DNA copy number differences between a reference genome a
Use-of-SemiThin-Cryosections-for-Light-Microscopy.
Use of Semi-Thin Cryosections for Light Microscopy.Semi-thin sections can be obtained from frozen blocks of cryoprotected biological material by secti
IHC全攻略2:如何制备样品
对于每一项IHC/ICC研究,组织和细胞样本的制备都不可掉以轻心。为了方便孵育,整个组织必须被切成超薄(5-10 μm)的切片,或切成小块用于整体免疫组化(whole mount IHC)。对于ICC实验,在开始染色步骤之前,细胞必须附着在显微镜载玻片或盖玻片上。样本制备也与固定方法密切
华大基因、中科院Nature子刊发表新成果
OpGen公司今日宣布,通过利用ARGUS® Whole Genome Mapping System技术结合新一代测序技术(NGS),生成了首个家山羊(domestic goat)的高质量参考基因组。这一研究是由深圳华大基因研究院和中科院昆明动物研究所领导,相关成果发表在《自然生物技术》
SMD集成电路的封装特点
SMD(surface mount devices)表面贴装器件。偶而,有的半导体厂家把SOP 归为SMD(见SOP)。SOP 的别称。世界上很多半导体厂家都采用此别称。(见SOP)。
Methylation-of-Fatty-Acids-(Kropinski-Method)
Methylation of Fatty Acids (Kropinski Method) OBJECTIVE: To methylate fatty acids in whole cells or lipopolysaccharide. REAGENTS : Methanol-Hyd
Fluorescence-Procedures-forthe-Actin-andTubulin-Cytoskeleton-in-Fixed-Cells
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed CellsActin: Louise CramerTubulin: Arshad DesaiGeneral StrategyWe typically wor
CCD-Camera介绍
CCD Camera(CCD Microscope Camera)是电荷耦合式摄像机的简称,主要功能为将来自镜头光学图象聚焦,经由CCD转换为随时间变化的视频信号,再经摄象机内部线路处理CCD转换视频信号,然后经由同轴或光缆或其它传输途径将信号传送至电脑经图象卡处理将信号在显示器上显示。 Cool
Plasma-and-Serum-Preparation
实验概要Serum is the liquid fraction of whole blood that is collected after the blood is allowed to clot. The clot is removed by centrifugation and the
Sample-preparation-(analytical-gels)
Sample preparation and solubilization are crucial factors for the overall performance of the 2-D PAGE technique. Protein complexes and aggregates shou
与骨骼相关的组织学实验技术方法
Embedding Bones for Frozen Section:Fill an ice bucket with dry ice pellets and place a 500ml beaker in the middle.Put approximately 200ml of 2-MethylB
Biosensis的神经肽及其受体研究相关抗体的应用(二)
艾美捷科技作为专业的生命科学领域解决方案供应商,为您推荐Biosensis品牌的可用于神经肽及其受体研究相关的抗体集合。Biosensis抗体可用于许多应用,包括ELISA,免疫印迹(WB),免疫组织组化(IHC),免疫细胞化学(ICC),免疫沉淀(IP),免疫荧光(IF)和流式细
Global-Expression-Profiling-of-RNA-from-Laser-Microdissected-Cells-at-...
Global expression profiling of RNA isolated from laser microdissected cells allows one to profile a specific set of cells allowing for enhanced s
Fluorescent-Staining-of-Cells
1. Fluorescent phalloidin in methanol. Phallacidin does not work as well. Dilute 10 ul 330 nM stock into 500 ul PBS for each large coverslip. 2.
AvaSpecDual-双通道型光纤光谱仪机箱
AvaSpec-Desktop/Rackmount 多通道机箱机箱类型 Desktop桌面型Rack-mount可安装型最大通道数 4个10个外型尺寸 315 x 235 x 135 mm315 x 445 x 135 mm AvaSpec-Dual 双通道机箱接口 2 x USB2.0 2 x