MethylationofFattyAcids(KropinskiMethod)
Methylation of Fatty Acids (Kropinski Method)OBJECTIVE:To methylate fatty acids in whole cells or lipopolysaccharide.REAGENTS :Methanol-Hydrochloride Reagent Kit>10mg of whole cells or 1 mg of lipopolysaccharide400 nmoles of fatty acid standard (pentadecanoic acids C15)METHODS:Prepare 1M MeOH-HCl reagent according to the instructiongiven with the kit.Add internal standard (C15 fatty acid) to give a final concentra......阅读全文
Methylation－of－Fatty－Acids－(Kropinski－Method)
Methylation of Fatty Acids (Kropinski Method)OBJECTIVE:To methylate fatty acids in whole cells or lipopolysaccharide.REAGENTS :Methanol-Hydrochloride
BetaOxidation－of－Fatty－Acids
Fatty acids provide highly efficient energy storage, storing much more energy for their weight than carbohydrates like glucose. Fatty acids are stored
Methylation－Specific－PCR
Methylation Specific PCRProtocol written by James Herman*Methylation Specific PCR (MSP) is a simple rapid and inexpensive method to determine the meth
Protein－arginine－methylation
Typical modification sites: RGG box or RXR sequence motifs R-arginine, G-glycine,X-any aminoacid.Enzymes catalysing protein arginine methylation: PRMT
Oxidation－of－Polyunsaturated－Fatty－Acid
Unsaturated fatty acids are those that contain one or more double bonds in their alkyl-chain. Polyunsaturated fats with two double bonds usually have
Mechanisms－of－transcriptional－repression－by－DNA－methylation
Tumorigenesis is known to be a multistep process in which defects in various cancer genes accumulate. Epigenetic modifications, most importantly DNA m
MethPrimer－－Design－Primers－for－Methylation－PCRs
Welcome to MethPrimer MethPrimer is a program for designing bisulfite-conversion-based Methylation PCR Primer. Currently, it can design primers for tw
Glucogenic－and－Ketogenic－Amino－Acids
A significant amount of metabolic energy can come from amino acid metabolism, particularly under conditions of starvation. The metabolism of amino aci
Mitochondrial－Carnitine－Palmitoyltransferase－(CPT)－System
The oxidation of fatty acids is an important source of energy for ATP production in mitochondria through the entry of acetyl-CoA into the Krebs cycle.
Comparison－of－Beta－oxidation－in－mitochondria－and－peroxisomes－andglyoxysomes
In addition to the beta-oxidation of fatty acids in mitochondria, animal cells carry out beta-oxidation in another organelle, the peroxisome. Although
Oxidation－ofoddnumbered－chain－fatty－acid－from－PropionylCoA－toSuccinylCoA
NON-Clickable Image The beta-oxidation of fatty acids in mitochondria progressively shortens fatty acids two-carbons at a time as acetyl-CoA units are
脂肪酸的分类依据
根据碳链长度的不同分类可分为：短链脂肪酸、中链脂肪酸和长链脂肪酸。脂肪酸代谢脂肪酸根据碳链长度的不同又可将其分为 ：短链脂肪酸（short chain fatty acids，SCFA），其碳链上的碳原子数小于6，也称作挥发性脂肪酸（volatile fatty acids，VFA）； 中链脂肪酸（
Basic－mechanism－of－action－of－PPARa,－PPARb(d)－and－PPARg－and－effects
Similar to other nuclear hormone receptores, PPAR acts as a ligand activated transcription factor. Upon binding fatty acids or hypolipidemic drugs, PP
nucleic－acids－research影响因子
《NUCLEIC ACIDS RESEARCH》是一个期刊，最新的影响因子为11.561。 NUCLEIC ACIDS RESEARCH 出版国家：England 出版商：Oxford University Press 出版周期：Semimonthly 语言：English 研究领域
Omega－Oxidation
NON-Clickable Image While the main route of fatty acid metabolism is through beta-oxidation, some minor metabolic pathways such as omega oxidation als
检测粪便中短链脂肪酸有什么意义
酸根据长度的不同又可将其分为短链酸（short chain fatty acids,SCFA），其碳链上的碳原子数小于6，也称作挥发性酸(volatile fatty acids,VFA）；中链酸（Midchain fatty acids，MCFA），指碳链上碳原子数为6-12的酸，主要成分是辛酸（
Lipid－analysis－Week－3:－GAS－LIQUID－CHROMATOGRAPHY
During this week, you will analyze the fatty acid composition of the individual lipid fractions recovered from the TLC plate. Gas chromatography is a
脂肪酸根据碳链长度的不同分类
可分为：短链脂肪酸、中链脂肪酸和长链脂肪酸。 脂肪酸根据碳链长度的不同又可将其分为： 短链脂肪酸（short chain fatty acids，SCFA），其碳链上的碳原子数小于6，也称作挥发性脂肪酸（volatile fatty acids，VFA）； 中链脂肪酸（Midchain
根据碳链长度对脂肪酸进行分类
可分为：短链脂肪酸、中链脂肪酸和长链脂肪酸。脂肪酸根据碳链长度的不同又可将其分为 ：短链脂肪酸（short chain fatty acids，SCFA），其碳链上的碳原子数小于6，也称作挥发性脂肪酸（volatile fatty acids，VFA）；中链脂肪酸（Midchain fatty ac
脂肪酸根据碳链长度的不同分类
可分为：短链脂肪酸、中链脂肪酸和长链脂肪酸。 脂肪酸根据碳链长度的不同又可将其分为： 短链脂肪酸（short chain fatty acids，SCFA），其碳链上的碳原子数小于6，也称作挥发性脂肪酸（volatile fatty acids，VFA）； 中链脂肪酸（Midchain f
Shuttle－for－transfer－of－acetyl－groups－from－mitochondria－to－the－cytosol
Acetyl-CoA is produced in mitochondria through the metabolism of fatty acids and the oxidation of pyruvate to acetyl-CoA. When ATP is needed, this ace
脂肪酸的分类，结构特点及存在形式
脂肪酸（fatty acid）是具有长链烃的羧酸，分为饱和脂肪酸（saturated fatty acid，SFA）；单不饱和脂肪酸（monounsaturated fatty acids，MUFA），其碳氢链有一个不饱和键不饱和脂肪酸。多不饱和脂肪酸（polyunsaturated fatty a
Assay－of－Phospholipase－A－Activity
Phospholipases of the A type constitute a large family of esterases that catalyze the hydrolysis of the fatty acid ester bonds in phospholipids an
Gelelongation－assay－for－type－II－fatty－acid－synthesis
Gel-elongation assay for type II fatty acid synthesisSrinivas KodaliAndrew GalgociSheo Singh Dr.Jun Wang Dr., jun_wang2@merck.com, Merck Research Labo
脂肪酸的分类依据及分类
脂肪酸有多种分类形式，分别分类如下。根据碳链长度的不同分类可分为：短链脂肪酸、中链脂肪酸和长链脂肪酸。脂肪酸根据碳链长度的不同又可将其分为 ：短链脂肪酸（short chain fatty acids，SCFA），其碳链上的碳原子数小于6，也称作挥发性脂肪酸（volatile fatty acids
亚麻酸的相关内容介绍
按照脂肪烃的饱和程度，即是否存在双键或二键（一般为双键），脂肪酸又可分为饱和脂肪酸（saturated fatty acids，SFA）和不饱和脂肪酸（unsaturated fatty acids，UFA），其中不饱和脂肪酸可以根据双键的多少分为单不饱和脂肪酸（mono-unsaturatcd
HSD17B4基因编码功能及结构描述
由该基因编码的蛋白质是参与脂肪酸过氧化物酶体β氧化途径的双功能酶。它还可以催化直链和2-甲基支链脂肪酸形成3-酮酰基-CoA中间体。影响过氧化物酶体脂肪酸β氧化活性的基因缺陷是D-双功能蛋白缺乏症（DPD）的一个原因。这个基因的一个明显的假基因存在于8号染色体上。已发现该基因的多个选择性剪接转录变体
HSD17B4基因编码功能及结构描述
由该基因编码的蛋白质是参与脂肪酸过氧化物酶体β氧化途径的双功能酶。它还可以催化直链和2-甲基支链脂肪酸形成3-酮酰基-CoA中间体。影响过氧化物酶体脂肪酸β氧化活性的基因缺陷是D-双功能蛋白缺乏症（DPD）的一个原因。这个基因的一个明显的假基因存在于8号染色体上。已发现该基因的多个选择性剪接转录变体
HSD17B4基因突变与药物因子介绍
由该基因编码的蛋白质是参与脂肪酸过氧化物酶体β氧化途径的双功能酶。它还可以催化直链和2-甲基支链脂肪酸形成3-酮酰基-CoA中间体。影响过氧化物酶体脂肪酸β氧化活性的基因缺陷是D-双功能蛋白缺乏症（DPD）的一个原因。这个基因的一个明显的假基因存在于8号染色体上。已发现该基因的多个选择性剪接转录变体
Phosphatidylcholine－Biosynthesis－Pathway
The main components of biological membranes are phosphoglyceride lipids composed of a glycerol unit esterified to two fatty acids and a polar alcohol