ChIPusingplantsamples–Arabidopsis
实验概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). The exact chromatin concentration should be determined before starting the X-ChIP assay. View our cross-linking chromatin immunoprecipitation (X-ChIP) protocol, which should be used after the chromatin preparation detailed below.实验原理Eukaryotic chromatin is a co......阅读全文
IMAGINGPAM相关文献集（三）
101. Li Q-M, Liu B-B, Wu Y, Zou Z-R. Interactive Effects of Drought Stresses and Elevated CO2 Concentration on Photochemistry Efficiency of C
Using－GenBank
GenBank(R) is a comprehensive database of publicly available DNA sequences for more than 205,000 named organisms and for more than 60,000 within t
EcoTech植物表型成像分析全面解决方案（三）
6) PlantScreen NIR成像采用多滤波器技术，不仅测量水分吸收峰值1450nm，还可进行reference测量如植物对1000nm反射，从而得到高反差水分分布信息。而其它产品近红外成像只有一个滤波器得到1400nm的反射影像，没有reference测量（对照参考测量），得到的数据很大
Protein－concentration－of－Laemmli－gel－samples
Protein concentration of Laemmli gel samplesTo 10 µl boiled lysate (in Laemmli sample buffer) add 40µl water + 50µl 50% TCA. Ppt. 10 min. on ice. Spin
选择性微电极在植物生理学研究中的应用（五）
7 展望选择性微电极技术能用于直接并灵敏地观察植物体对矿质元素的需求，研究者可利用选择性微电极技术进行对植物某种离子或高或低的吸收的品种的筛选，还可制定出与植物需求相适应的环境的营养水平；能及时准确地探测到的光、温、水涝、盐分引起的植物体离子或分子信息的微小变化，能成为预测植物受到逆境胁迫最直观、
Crosslinking－chromatin－immunoprecipitation－(XChIP)－protocol
实验概要ChIP is a powerful tool that allows the specific identification of proteins or histone modifications to regions of the genome. Chromatin is isol
Fluidigm公司微液流芯片在单细胞研究中的应用（二）
It was also worth it for Tannishtha Reya, who studies stem cell fate and cancer at Duke University Medical Center in Durham, North Carolina. "Im
Cloning－of－small－RNAs－with－5’－phosphate－and－3’－OH－ends01
IntroductionThe following protocol describes a procedure for the purification and cloning of miRNAs and other small RNAs in the 20-30 nucleotide size
Production－of－Antibody－Fragments－in－Arabidopsis－Seeds
Plants offer a number of attractive benefits over conventional mammalian or bacterial cell culture systems for the production of valuable pharmace
Using－a－Counting－Chamber
Using a Counting ChamberFor microbiology, cell culture, and many applications that require use of suspensions of cells it is necessary to determine ce
ChIPChip技术的介绍与应用
人类基因组计划的完成开启了一个新的纪元——功能基因组时代来临，与基因信息相比较，人们更关注于基因的功能、调控网络与信号通路等信息。表观遗传学研究与核内蛋白因子的功能分析成为基因表达调控研究的重要组成部分。结合了染色质免疫共沉淀与基因芯片技术的ChIP-chip技术的浮现使得全基因组范围内DNA与蛋白
Measurement－of－Carbon－Fixation－Rates－in－Leaf－Samples
Generation of a Light Curve To address the hypothesis concerning photosynthetic efficiency it is necessary to expose sun and shade leaves to a ran
CORE－SAMPLE－PCR:－A－method－to－rePCR－unique－bands－from－products－of－mixed－s
INTRODUCTIONThe products of a PCR reaction - especially when this is done on eukaryotic genomic DNA, and when using degenerate primers - often contain
IMAGINGPAM相关文献集（四）
151. 邓培雁, 刘威, 韩博平. 宝山堇菜（Viola baoshanensis）镉胁迫下的光合作用 生态学报,2007, 27(5):1858-1862. 152. 邓培雁, 刘威, 韩博平, 韩志国. 宝山堇菜（Viola baoshanensis）、紫花地丁(V. y
核蛋白基因组指纹技术
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
早期胚胎发育中的单胚胎细胞基因表达（二）
“We picked 42 genes to validate on the BioMark system,” Dr. Yao said. “We picked them to represent different functional categories.”“We used the F
Streamlined－DNA－Extraction－Protocol
This method is derived from a procedure developed by Toby Bradshaw and the Poplar Molecular Genetics Cooperative. We have tested the procedure wit
CORE－SAMPLE－PCR
A method to re-PCR unique bands from products of mixed sizeContentsINTRODUCTIONPROTOCOLCOMMENTSINTRODUCTIONThe products of a PCR reaction - especially
IMAGINGPAM相关文献集（二）
51. Krupenina NA, Bulychev AA. Action potential-induced changes in photosynthetic pattern of a plant cell Comparative Biochemistry and Phys
碳水化合物分析
Carbohydrate Assay (Hancock Laboratory) (Accessible only by IE)This protocol is used to determine the relative amounts of LPS CHO present in a given s
Deglycosylation－of－Glycoproteins－Using－Endoglycosidases
T.H. Plummer, Jr. and A.L. Tarentino, Department of Biochemistry, New York State Department of Health, Wadsworth Center for Laboratories and Research,
利用植物表型组学挖掘基因组学的成果
到2050年，全球人口将达到97亿，预计作物产量翻一番才能满足全球人口的粮食需求。为了达到这一目标，作物产量需每年增长2.4%，但目前作物产量平均增长率仅为1.3%。作物生产性能的遗传改良仍然是提高作物生产力的关键因素，但当前的改善速度无法满足可持续性和粮食安全的需要。为了确保粮食安全、生态系统的可
IMAGINGPAM相关文献集（一）
德国WALZ公司生产的M系列调制叶绿素荧光成像系统IMAGING-PAM，作为全球最权威的叶绿素荧光成像系统，在国际科研界得到了广泛应用，已经成为叶绿素荧光成像研究的行业标准。 泽泉科技有限公司作为德国WALZ在中国的技术服务中心，一直以来致力于推广调制叶绿素荧光技术在国内科研界的应用，并
早期胚胎发育中的单胚胎细胞基因表达（一）
Single-embryo Gene Expression for Early Embryo DevelopmentMylene Yao, M.D. Assistant ProfessorDept. of Obstetrics and Gynecology Stanford UniversityMy
染色质免疫沉淀芯片（ChipChip）
该技术能够快速在目标基因组的染色体中确定特异DNA结合蛋白的准确结合位点，ChIP芯片也可以在一个基因组的任何感兴趣的区域内寻找染色体的结构改变。一、ChIP-Chip的用途（1）在基因组范围内确定基因转录因子的DNA结合位点和其他DNA结合蛋白或蛋白复合体的DNA结合位点。（2）染色体活性状态的定
FluorCam多光谱荧光成像技术应用案例——植物干旱响应表...
FluorCam多光谱荧光成像技术应用案例——植物干旱响应表型研究植物对干旱的响应过程非常复杂，同时植物也有多样的应答机制来回避和耐受干旱胁迫并维持生长。光合系统被认为是对干旱极为敏感的，因此FluorCam叶绿素荧光成像系统从问世起就被广泛应用于植物干旱胁迫的研究。美国怀俄明大学将芜菁Brassi
Insect－DNA－Isolation－Protocol
实验概要The E.Z.N.A.® Insect DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from insects, arthropods, roundworms, flatw
Adventitious－Root－Induction－in－Arabidopsis－thaliana－as－a－Model－for－In...
Adventitious root formation, the development of roots on non-root tissue (e.g. leaves, hypocotyls and stems) is a critical step during micropropag
Green－lab－protocol－for－vacuum－infiltration－transformation－of－Arabidopsis
This protocol is adapted from protocols by Nicole Bechtold (Bechtold et al. 1993), Andrew Bent (Bent et al. 1994) and Takashi Araki. No claims are
Assay－of－superoxide－dismutase－activity3
Botanical Bulletin of Academia Sinica, Vol. 37, 1996The method using NBT as a superoxide radical competitor and a color indicator was also explored to