UsingaCountingChamber
Using a Counting ChamberFor microbiology, cell culture, and many applications that require use of suspensions of cells it is necessary to determine cell concentration. One can often determine cell density of a suspension spectrophotometrically, however that form of determination does not allow an assessment of cell viability, nor can one distinguish cell types.A device used for cell counting is called a counting cham......阅读全文
Using－a－Counting－Chamber
Using a Counting ChamberFor microbiology, cell culture, and many applications that require use of suspensions of cells it is necessary to determine ce
Chemotaxis－Assay－Using－Microchemotaxis－Chambers－(Modified－Boyden－Chamber)
Overview This assay uses a simple apparatus to study chemotaxis of leukocytes or other migratory cells. The apparatus consists of two multi-well chamb
发育生物学
In Vitro Production of Bovine Embryos (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)This protocol describe procedures for in vitro
Cell－counting/plating
OverviewCount the number of viable cells in a culture via dilution plating. The following assumes E. coli cells but it should apply to any type of cel
Measurement－of－Carbon－Fixation－Rates－in－Leaf－Samples
Generation of a Light Curve To address the hypothesis concerning photosynthetic efficiency it is necessary to expose sun and shade leaves to a ran
Cell－counting－with－an－hemacytometer.
Accuracy of manual counts with an hemacytometer depend on:accurate mixing of the sample (no bubbles!)number of chambers countednumber of cells counted
Counting－ES－cell－Chromosomes
(original reference: "tissue culture made easy" by Christian LaMantia from the Magnuson lab)1) Plate cells onto gelatinized plates (35 or 60 mm) witho
细胞培养常规操作
常规操作(主要内容如下)· Aseptic Technique· Culture Vessels· Cell Counting· Primary Culture· Maintenance of Cell Line ·
细胞计数的多种方法
A hemacytometer (also spelled hemocytometer) is an etched glass chamber with raised sides that will hold a quartz coverslip exactly 0.1 mm above the c
Use－of－a－Hemacytometer
A hemacytometer (also spelled hemocytometer) is an etched glass chamber with raised sides that will hold a quartz coverslip exactly 0.1 mm above the c
Method:－Preparation－of－Lymphoblastoid－Cell－Lines－for－Long－Term－Storage
Method: Preparation of Lymphoblastoid Cell Lines for Long Term StorageMay 30, 1990Rosalie VeilePurpose:To store cell lines in a form that will insure
Using－GenBank
GenBank(R) is a comprehensive database of publicly available DNA sequences for more than 205,000 named organisms and for more than 60,000 within t
Cell－Counting/－LiveDead－Discrimination
This is a microscopy based application for definitive discrimination of live and dead cells. Trypan Blue exclusion notoriously over estimates the numb
Critical－Appraisal－of－the－MTT－Assay－in－the－Presence－of－Rottlerin－2
Materials and methodsMaterials All chemicals and materials for cell culture (unless otherwise indicated) were obtained from Sigma (Milan, Italy). La
Dynamic－Flow－Assay－in－a－Parallel－Plate－Flow－Chamber
Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850Flow assays allow visualization of c
Deglycosylation－of－Glycoproteins－Using－Endoglycosidases
T.H. Plummer, Jr. and A.L. Tarentino, Department of Biochemistry, New York State Department of Health, Wadsworth Center for Laboratories and Research,
ChIP－using－plant－samples
实验概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin immunoprecipitati
Detection－and－Measurement－of－Radioactivity
Liquid scintillation countingThe amount of kinetic energy in a beta particle differs from one decay to the next. However, each radioisotope has a typi
MiniChamber－for－Regulating－Gaseous－Environment－During－Culture
1. The basic unit is constructed from a media filtration unit such as Corning’s 115 ml filter system (catalog numbers 430944-430947).2. Tygon tubing (
双通道尤斯灌流室（Ussing－Chamber）
UC-2M双通道尤斯室（尤斯灌流室）基座用于安装各种规格的尤斯室。尤斯室（尤斯灌流室）及其基座共同组成离体组织体外实验装置。将上皮组织安装在尤斯室中，尤斯室内加入生理溶液，并利用基座恒温和通氧，可使离体的上皮组织在较长时间内继续保持生理活性，以便研究者对上皮功能进行体外研究。 UC-2M是
双通道尤斯灌流室（Ussing－Chamber）
UC-2M双通道尤斯室（尤斯灌流室）基座用于安装各种规格的尤斯室。尤斯室（尤斯灌流室）及其基座共同组成离体组织体外实验装置。将上皮组织安装在尤斯室中，尤斯室内加入生理溶液，并利用基座恒温和通氧，可使离体的上皮组织在较长时间内继续保持生理活性，以便研究者对上皮功能进行体外研究。 UC-2M是
MiniChamber－for－Regulating－Gaseous－Environment－During－Culture
Mini-Chamber for Regulating Gaseous Environment During Culture R.M. Rivera and P.J. HansenA simple and inexpensive chamber for regulating gaseous envi
血球计数板计数误差的来源及解决方案—自动化计数
引言血球计数板一直是实验室细胞计数的金牌标准。自从18世纪在法国第一次被用于分析病人的血液样本，血球计数板在过去几百年中已经得到一系列的重大发展，相比以前计数更为精确、使用更为简单，并最终形成了今天我们使用的样子。现在血球计数板计数仍然是所有细胞学研究的一个组成部分，然而其计数存在的问题由于自身固有
Transfection－of－Mammalian－Cells－Using－Lipofectamine
Materials: LipofectamineBasal Medium containing 10% fetal bovine serum, 1% glutamine, 1% aaBasal Medium containing 1% glutamineBasal Medium cont
Method:－Cell－Counts－Using－a－Hemacytometer
Method: Cell Counts Using a HemacytometerJune 1, 1990Rosalie VeilePurpose:The purpose of this procedure is to determine the cell density of the cultur
Using－GenBank－for－Genomic－Authentication:－A－Tutorial
The GenBank database is perhaps one of the most important repositories of genetic information. A researcher working in the field of genomic authe
ChIP－using－plant－samples－–－Arabidopsis
实验概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). T
Extraction－of－DNA－using－DNAzol®－Reagent
实验概要DNAzol® Reagent (Genomic DNA Isolation Reagent) is a complete and ready-to-use reagent for the isolation of genomic DNA from solid and liquid sa
Fluorescence－In－Situ－Hybridization－using－TSA™
实验概要This protocol describes steps for fluorescent in situ hybridization (FISH) to Drosophila embryos using Tyramide Signal Amplification (TSA™), and
RNA－extraction－using－trizol/tri
RNA extraction with TRIzol (Invitrogen product name) or the equivalent TRI (Sigma-Aldrich product name) is a common method of total RNA extraction fro