EZ96®M13IsolationVacuumManifoldProtocol
实验概要The E.Z.N.A.™ family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources. The key to this system is Omega Bio-Tek, Inc.’s (OBI) new HiBind® matrix that specifically, but reversibly, binds DNA or RNA under certain optimal conditions, while allowing proteins and other contaminants to be removed. ......阅读全文
EZ－96®－M13－Isolation－Vacuum－Manifold－Protocol
实验概要The E.Z.N.A.™ family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a vari
EZ－96®－M13－Isolation－Vacuum－Manifold－Protocol
实验概要The E.Z.N.A.™ family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a vari
EZ－96®－M13－Isolation－Spin－Protocol
实验概要The E.Z.N.A.™ family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a vari
Nucleolar－Isolation－Protocol
We recommend that you first download and read this page as a PDF file. Using that as your guide, you can then follow the protocol below and view a Qui
RNA－Isolation－Protocol
RNA Isolation Protocol(Revised 5-15-2003)Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109 cells (OD600= 0.2 Dilute cells or scale up)
RNA－Isolation－Protocol
Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109 cells (OD600= 0.2 Dilute cells or scale up)Pipet 30 ml of RNAProtect Bacteria Reagen
Vacuum/Spin－Protocol－for－Tissue－DNA－Extraction
实验概要The E.Z.N.A.® Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysis. Up to 3
Green－lab－protocol－for－vacuum－infiltration－transformation－of－Arabidopsis
This protocol is adapted from protocols by Nicole Bechtold (Bechtold et al. 1993), Andrew Bent (Bent et al. 1994) and Takashi Araki. No claims are
Fastfilter－Plasmid－Midi－Kit－Vacuum/Spin－Protocol
实验概要The E.Z.N.A.TM Fastfilter Plasmid Midi Kit combines the power of HiBind® technology with the time-tested consistency of alkaline-SDS lysis of
Insect－DNA－Isolation－Protocol
实验概要The E.Z.N.A.® Insect DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from insects, arthropods, roundworms, flatw
Cajal－Body－Isolation－Protocol
Buffers and solutions(All solutions are supplemented with Complete Protease inhibitor tablet (Roche, Cat no: 1-873-580) at the final concentration of
重组DNA的分离、克隆与测序实验手册8
B. Midiprep double-stranded DNA isolationA midi-prep double-stranded DNA isolation has been developed to generate a sufficient amount of template DNA
Vacuum/Spin－Protocol－for－Isolating－DNA－from－Blood－with－Nucleated－Red
实验概要DNA isolation from fish or avian blood sample can be difficult because it contains nucleated red blood cells. E.Z.N.A. NRBC Blood DNA Kit is de
Nucleolar－Isolation－Protocol2
7. Resuspend the pellet with 3 ml S1 solution (Figure 3). The pellet should be resuspended readily by pipetting up and down. A pellet that cannot be r
E.Z.N.A.®－Plasmid－Maxi－Kit－vacuum－Protocol
实验概要This Protocol is designed to isolate 500-1200 ug of high Copy-Number plasmids or 50-400 ug of low Copy-Number Plasmids from 200 ml overnight c
E.Z.N.A.™－Fastfilter－Plasmid－Mega－Protocol
实验概要The E.Z.N.A.™ family of products is an innovative system that radically simplifies extraction and purification of nucleic acids from a variety
DNA－Purification－from－Blood－or－Body－Fluids
实验概要试剂盒，操作简单，稍有点贵，还可以接受。实验材料blood实验步骤Protocol: DNA Purification from Blood or Body Fluids(Spin Protocol)This protocol is for purification of total (ge
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
Jacobs:Protocol－Total－Protein－Isolation－Using－RIPA－Lysis－Buffer
MaterialsRIPA buffer (RIPA buffer enables the extraction of cytoplasmic, membrane and nuclear proteins and is compatible with many applications, inclu
DNA抽提
DNA抽提（主要内容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction from Cell and Tissue· Mitochondria DNA Isola
Fastfilter－Plasmid－Midi－Kit－Spin－Protocol
实验概要The E.Z.N.A.TM Fastfilter Plasmid Midi Kit combines the power of HiBind® technology with the time-tested consistency of alkaline-SDS lysis of
Protocol－for－Trichloroacetic－Acid－(TCA)－Precipitation
IntroductionThe efficiency of nucleotide incorporation in DNA/RNA polymerization reactions (e.g. transcription, reverse transcription, and DNA replica
Protocol－for－Trichl...
实验概要The efficiency of nucleotide incorporation in DNA/RNA polymerization reactions (e.g. transcription, reverse transcription, and DNA replication)
96Well－Sample－Preparation－for－Adherent－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB/c 3T3, et
96Well－Sample－Preparation－for－Adherent－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB/c 3T3, et
M13噬菌体
· M13 Phage (Michael Blaber)Very useful background information about M13: its infection, replication, packing, cloning. If you are new to phag
其它PCR方法
· Standard PCR Protocol (Molecular Biology Techniques Manual)The followings are described in detailRecommended Reagent ConcentrationsRecommend
线虫总RNA提取及RTPCR实验方法
C. elegans RNA Isolation and RT-PCRReagents Needed:M9 (common stock)Trizol (stored at 4ºC)chloroform2-propanol70% EtOHRNase-free H2OiScript cDNA Synth