DecontaminationofEthidiumBromideSolutionsandSurfaces
WARNING: EtBr is toxic and mutagenic. Hypophosphorus and its solutions are\corrosive. Decontamination solution gives off a small amount of nitrogendioxide, a toxic gas, when initially mixed.Laboratory Safety Practices and Equipment:-Prepare decontamination solution in the fume hood.-Wear two layers of gloves, lab coat and safety glasses.-Turn off electrical equipment before decontamination.Preparation of Decontaminat......阅读全文
Decontamination－of－Ethidium－Bromide－Solutions－and－Surfaces
WARNING: EtBr is toxic and mutagenic. Hypophosphorus and its solutions are\corrosive. Decontamination solution gives off a small amount of nitrogendio
Ethidium－Bromide－Decontamination
N.B.: Ethidium bromide is a powerful mutagen. Protective gloves should be worn at all times when handling solutions containing ethidium bromide.Decont
Ethidium－bromide
Introduction Ethidium bromide (EtBr), 3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide, is commonly used as a non-radioactive marker for identify
溴乙非啶的处理
N.B.: Ethidium bromide is a powerful mutagen. Protective gloves should be worn at all times when handling solutions containing ethidium bromide.Decont
10mg/ml的溴化乙锭（ethidium－bromide）
小心称取1g溴化乙锭，转移到广口瓶中，加100ml水，用磁力搅拌器搅拌直到完全溶解。用铝箔包裹装液管，于4℃贮存。
核酸纯度、浓度与分子量测定实验——Ethidium－bromide染色法
实验方法原理利用一系列不同浓度的DNA标准溶液（0、2.5、5、10、20、30、40、50 ng/ml），或已知浓度的DNA marker，和未知浓度DNA样品一起进行琼脂糖凝胶电泳，以EB染色后，在凝胶图象分析仪上观察，比较标准浓度及未知浓度的亮度，来求取DNA 的含量；比较DNA样品与DNA
Standard－neutral－agarose－electrophoresis
Standard neutral agarose electrophoresisStandard agarose gels can be prepared using either TBE or TAE running buffers.You will need:Either 10 x TBE or
对比法测定DNA浓度
Plate assay for determination of DNA concentrationA fairly accurate, rapid assay of DNA concentration can be obtained by UV visualization of samples s
General－Laboratory－Procedures,－Equipment－Use,－and－Safety－Considerations
A. Storage .The following properties of reagents and conditions are important considerations in processing and storing DNA and RNA. Heavy metals promo
ELECTROPHORESIS－OF－DNA－IN－AGAROSE－GELS
ELECTROPHORESIS OF DNA IN AGAROSE GELSA). AGAROSE CONCENTRATIONS: Use 0.8% agarose (w/v) for high molecular weight DNA fragments, and 1 - 1.2% f
Decontamination－of－cells－from－the－yeast
I Destroy yeast1. Aspirate medium and wash cell in PBS.2. Incubate cells at 37oC for 5 min in non-diluted antibiotic-antimycotic.3. In
基本实验技术
I. Safety ProceduresA. ChemicalsA number of chemicals used in this laboratory are hazardous. All manufacturers of hazardous materials are required by
质粒的大量制备
· Plasmid Mini and Maxi Prep Methods (Gimila Lab) · Maxi-preps and all media, solutions (NWFSC)Isolation of cosmid, plasmid and P1 DNA
质粒的大量制备
· Plasmid Mini and Maxi Prep Methods (Gimila Lab) · Maxi-preps and all media, solutions (NWFSC)Isolation of cosmid, plasmid and P1 DNA
RNA－gel－electrophoresis
MaterialsDEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37ºC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide solutions should also be
Cyanogen－Bromide－digestion－of－protein
1. Proteins are TCA precipitated and washed with acetone, then dried.2. The CNBr should be brought to room temperature in the hood and used ONLY in th
RNA－gel－electrophoresis
实验概要RNA gel electrophoresis主要试剂DEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37ºC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide sol
实验常用试剂的制备与储藏（三）
二.电泳缓冲液、染料和凝胶加样液 电泳缓冲液 50×Tris-乙酸（TAE）缓冲液 5×Tris-硼酸（TBE）缓冲液 染料 1％溴酚蓝（bromophenol blue） 加1g水溶性钠型溴酚蓝于100ml水中，搅拌或涡旋混合直到完全溶解。 1％二甲苯青FF（xylene
过氧化氢蒸汽－(－H－P－V－)－生物有效性（二）
2. 参考文献/ 出处1. Rogers, J. V., C. L. Sabourin, Y. W. Choi, W. R. Richter, D. C. Rudnicki, K. B. Riggs, M. L. Taylor, and J. Ch a ng.2005. Decontaminat
DNA提取中EB的去除实验方法
Removal of Ethidium Bromide from DNA by Extraction with Organic SolventsJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourn
Denaturing－Agarose－Gel－Electrophoresis－of－RNA
The overall quality of an RNA preparation may be assessed by electrophoresis on a denaturing agarose gel; this will also give some information about R
Solutions－for－Sea－Urchin－Development
Solutions for Sea UrchinASW: Artificial sea water (MBL)NaCl24.72 gKCl0.67 gCaCl2.2H2O1.36 gMgCl2.6H2O4.66 gMgSO4.7H2O6.29 gNaHCO30.18 gdH2Oto 1 literS
Cell－Culture－Media－and－Solutions
Antimycotic/antibiotic media:To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add:165.0 ml fetal bovine serum, heat inactivated12.0 ml 200mM (100
Stock－solutions－for－tissue－culture
The kitchen makes Tris, TD, Tryp/TD, PBS, and VE.Tris is a fairly complex, Tris-buffered physiological saline solution. It is used to wash cells and i
Cell－Culture－Media－and－Solutions
Cell Culture Media and SolutionsDec. 18, 1990R. VeileAntimycotic/antibiotic media:To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add:165.0 ml f
Bacterial－Media－Solutions－and－Stocks
3 agar (200 ml)Add 6 grams agar to 200 ml deionized water. Autoclave to sterilize.1.6 agar (200 ml)Add 3.2 grams agar to 200 ml deionized water.Autocl
Yeast－Media,－Solutions－and－Stocks
Yeast Media:Note: Synthetic complete medium can be prepared by adding media supplements (see below).Medium using 6.7 g yeast nitrogen base without ami
AMNIOTIC－FLUID－CULTURES－ON－COVERSLIPS
I. Purpose:Amniotic fluid may be used for prenatal diagnosis of aneuploidy or other structural abnormalities. II. Culture Procedure:A. Aseptic techniq
AMNIOTIC－FLUID－CULTURES－ON－COVERSLIPS
实验概要 AMNIOTIC FLUID CULTURES ON COVERSLIPS主要试剂Solutions:Colcemid working solution: 10 mcg/ml Colcemid in Hank's Balanced Salt Solution, sto
DIRECT－AND－SHORTTERM－PROCEDURE－FOR－HARVESTING－BONE－MARROW－CHROMOSOMES
I. PurposeTo identify chromosome anomalies in hematopoietic cells. Used especially for chromosome studies for hematological disorders such as preleuke