核酸纯度、浓度与分子量测定实验——Ethidiumbromide染色法
实验方法原理利用一系列不同浓度的DNA标准溶液（0、2.5、5、10、20、30、40、50 ng/ml），或已知浓度的DNA marker，和未知浓度DNA样品一起进行琼脂糖凝胶电泳，以EB染色后，在凝胶图象分析仪上观察，比较标准浓度及未知浓度的亮度，来求取DNA 的含量；比较DNA样品与DNA marker条带位置，推知DNA 样品分子量。实验材料DNA试剂、试剂盒琼脂糖TBE电泳缓冲液TE buffer溴化乙锭（EB）载样缓冲液仪器、耗材电泳仪电泳槽电子天平移液器枪头点样板微波炉紫外分光光度仪凝胶图象分析仪实验步骤1. 称取1.5 g 琼脂糖加入盛有100 ml 0.5xTBE电泳缓冲液三角瓶中，摇匀，在微波炉上加热至琼脂糖完全溶解。加入溴化乙锭（EB）至终浓度0.5 ug/ml，并摇匀。 2. 用胶带将制胶板两端封好，插入适当梳子，将溶解的琼脂糖倒入，室温冷却凝固。 3. &n......阅读全文
Ethidium－bromide
Introduction Ethidium bromide (EtBr), 3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide, is commonly used as a non-radioactive marker for identify
Ethidium－Bromide－Decontamination
N.B.: Ethidium bromide is a powerful mutagen. Protective gloves should be worn at all times when handling solutions containing ethidium bromide.Decont
Decontamination－of－Ethidium－Bromide－Solutions－and－Surfaces
WARNING: EtBr is toxic and mutagenic. Hypophosphorus and its solutions are\corrosive. Decontamination solution gives off a small amount of nitrogendio
10mg/ml的溴化乙锭（ethidium－bromide）
小心称取1g溴化乙锭，转移到广口瓶中，加100ml水，用磁力搅拌器搅拌直到完全溶解。用铝箔包裹装液管，于4℃贮存。
核酸纯度、浓度与分子量测定实验——Ethidium－bromide染色法
实验方法原理利用一系列不同浓度的DNA标准溶液（0、2.5、5、10、20、30、40、50 ng/ml），或已知浓度的DNA marker，和未知浓度DNA样品一起进行琼脂糖凝胶电泳，以EB染色后，在凝胶图象分析仪上观察，比较标准浓度及未知浓度的亮度，来求取DNA 的含量；比较DNA样品与DNA
Standard－neutral－agarose－electrophoresis
Standard neutral agarose electrophoresisStandard agarose gels can be prepared using either TBE or TAE running buffers.You will need:Either 10 x TBE or
对比法测定DNA浓度
Plate assay for determination of DNA concentrationA fairly accurate, rapid assay of DNA concentration can be obtained by UV visualization of samples s
ELECTROPHORESIS－OF－DNA－IN－AGAROSE－GELS
ELECTROPHORESIS OF DNA IN AGAROSE GELSA). AGAROSE CONCENTRATIONS: Use 0.8% agarose (w/v) for high molecular weight DNA fragments, and 1 - 1.2% f
DNA提取中EB的去除实验方法
Removal of Ethidium Bromide from DNA by Extraction with Organic SolventsJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourn
Denaturing－Agarose－Gel－Electrophoresis－of－RNA
The overall quality of an RNA preparation may be assessed by electrophoresis on a denaturing agarose gel; this will also give some information about R
溴乙非啶的处理
N.B.: Ethidium bromide is a powerful mutagen. Protective gloves should be worn at all times when handling solutions containing ethidium bromide.Decont
General－Laboratory－Procedures,－Equipment－Use,－and－Safety－Considerations
A. Storage .The following properties of reagents and conditions are important considerations in processing and storing DNA and RNA. Heavy metals promo
重组DNA的分离、克隆与测序实验手册7
III. Methods for DNA isolationA. Large scale double-stranded DNA isolationThe method used for the isolation of large scale cosmid and plasmid DNA is a
QUALITATIVE－ANALYSIS－OF－DNA－FRAGMENTATION－BY－AGAROSE－GEL－ELECTROPHORESIS
1. IntroductionNuclear morphology changes characteristic of apoptosis appear within the cell together with a distinctive biochemical event: the endonu
Preparation－of－Agarose－Gels－for－DNA－separations
Weigh out the desired amount of agarose and place in an Erlenmeyer flask with a measured amount of electrophoresis buffer, e.g. for an 0.8% gel, add 0
甲醛洋菜胶体电泳实验
甲醛洋菜胶体电泳 实验方法原理 rRNA 占细胞RNA总量的80~85%,以ethidium bromide 染色后，呈现于胶体上的两个主要RNA色带应该
甲醛洋菜胶体电泳实验
实验方法原理 rRNA 占细胞RNA总量的80~85%,以ethidium bromide 染色后，呈现于胶体上的两个主要RNA色带应该分别是large 与small rRNAs （真核生物为28S 与18S,原核生物为23S 与16S）；散布于small rRNA 附近，呈淡淡smear
溴化乙锭的基本信息
中文名溴化乙锭外文名Ethidium bromide别 名EB化学式C21H20BrN3分子量394.32CAS登录号1239-45-8EINECS登录号214-984-6熔 点261 ℃水溶性40g/L外 观粉末应 用荧光染色剂
甲醛洋菜胶体电泳实验
甲醛洋菜胶体电泳 （formaldehyde-agarose gel electrophoresis）甲醛是一种常用的RNA 变性剂。用于（1）RNA的分离测定（2）RNA提纯。实验方法原理rRNA 占细胞RNA总量的80~85%,以ethidium bromide 染色后，呈现于胶体上的两个主要R
electrophoresis－of－DNA
Agarose Gel Electroporesis of DNA Making the gel: 1. Place casting platform with well former sideways in gel stand where you wish to pour
Agarose－gel－electrophoresis
General ProcedureCast a gelPlace it in gel box in running bufferLoad samplesRun the gelImage the gelCasting Gels0.7% agarose gel with 1kbp ladder in U
凝胶电泳仪使用方法
该技术操作简便快速，可以分辨用其它方法（如密度梯度离心法）所无法分离的DNA片段。当用低浓度的荧光嵌入染料溴化乙啶(Ethidium bromide, EB)染色,在紫外光下至少可以检出1-10ng的DNA条带,从而可以确定DNA片段在凝胶中的位置。此外,还可以从电泳后的凝胶中回收特定的DNA条带,
凝胶电泳仪的使用方法
该技术操作简便快速，可以分辨用其它方法（如密度梯度离心法）所无法分离的DNA片段。当用低浓度的荧光嵌入染料溴化乙啶(Ethidium bromide, EB)染色,在紫外光下至少可以检出1-10ng的DNA条带,从而可以确定DNA片段在凝胶中的位置。此外,还可以从电泳后的凝胶中回收特定的DNA条
RNA－gel－electrophoresis
MaterialsDEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37ºC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide solutions should also be
DNA－mobility－in－gels
1. Migration of marker dyes in native polyacrylamide non-denaturing gels Gel % Bromophenol blue (BP) Xylene cyanole (XC) 3.5 100 460 5.0
DNA转化实验指导2
1B. Cloning 1. A caveat on dephosphorylation: the most common reason for failure to obtain colonies is a result of adding too much BAP or CIP to
4′,6二脒基2苯基吲哚的染色剂的染色原理
可用于细胞核染色的试剂是一种可以穿透细胞膜的蓝色荧光染料。和双链DNA结合后可以产生比DAPI自身强20多倍的荧光。和EB（ethidium bromide）相比，对双链DNA的染色灵敏度要高很多倍。DAPI染色常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核
RNA－gel－electrophoresis
实验概要RNA gel electrophoresis主要试剂DEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37ºC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide sol
Cell－Counting/－LiveDead－Discrimination
This is a microscopy based application for definitive discrimination of live and dead cells. Trypan Blue exclusion notoriously over estimates the numb
Agarose－Gel－Electrophoresis－of－DNA
1) Dissolve 1 g of agarose in 100 ml of 1X TAE or TBE buffer (gives a 1% gel). See note for making LMP agarose gel. 2) Cast the gel with the comb in p