GuidelinesfortheUseofAnalgesicsandTranquilizersinLaboratoryAnimal
What is Anesthesia? Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are analgesia (pain relief), amnesia (loss of memory) and immobilization. The drugs used to achieve anesthesia usually have varying effects in each of these areas. Some drugs may be used individually to achieve all three. Others have only analgesic or sedative properties ......阅读全文
PCR产物纯化方法
Purification of PCR Products in Preparation for CloningJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid
SMEAR－PREPARATION
The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria
Activation－and－Expansion－of－Human－Treg－Cells
实验概要This protocol is intended for activation and expansion of human Treg cells isolated with the Dynal® CD4 CD25 Treg Kit (Cat. no. 113.23D). The exp
LCM－PROTOCOLS
Slide SectioningParaffin blocks- For DNA analysis:Special LCM processing schedule is followed.The water bath is cleaned using RNAse Zap™, rinsed thoro
放射性同位素使用规则
RULES FOR THE USE OF RADIOACTIVITY You must be certified by EHS before you can use radioactivity. The guiding principle isCOMMON SENSE. I take radio
The－iPhyClassifier,－an－Interactive－Online－Tool－for－Phytoplasma－...
The i PhyClassifier is an internet-based research tool for quick identification and classification of diverse phytoplasmas. The i PhyClassifier simu
BJUT－and－H.E.L.－Joint－LaboratoryCollaborative－Excellence－for－a－Bright－Future
By the end of 2023, the number of new energy vehicles in China reached 20.4 million； whether it's power batteries or new energy vehicles, China
Preparation－of－Mitochondria－from－Rat－Liver
Preparation of Mitochondria from Rat LiverRat liver is an ideal source for functional intact mitochondria for a number of reasons. We use Sprague-Dawl
Protocol－for－Construction－of－BAC－Libraries
Protocol for Construction of BAC Libraries The bacterial artificial chromosome cloning (BAC) system is emerging as the system of choice for const
Horizon和Waters公司推出水分析的新型样品制备技术
Oasis® HLB萃取盘为环境实验室创造了更高的效率 简介：Salem, NH, 2008年11月3日—— Horizon技术公司和Waters公司推出了Oasis HLB萃取盘，一种用于EPA 8270和1694方法分析的固相萃取产品。 Horizon Technology and Wate
英国血液学会发布最新血浆输注指南
英国血液学会（BSH）近期发布了输注新鲜冰冻血浆（FFP）和冷沉淀血制品的最新指南。 新的血浆输注指南使用了推荐分级的评估、 制定与评价方法（GRADE），数据来源于2004年至2016年发表的文献。指南内容包括FFP和冷沉淀的安全解冻和储存及在无出血的患者中的使用方案。 指南
英国血液学会发布最新血浆输注指南
英国血液学会（BSH）近期发布了输注新鲜冰冻血浆（FFP）和冷沉淀血制品的最新指南。 新的血浆输注指南使用了推荐分级的评估、 制定与评价方法（GRADE），数据来源于2004年至2016年发表的文献。指南内容包括FFP和冷沉淀的安全解冻和储存及在无出血的患者中的使用方案。 指南
Genomic－DNA－Extraction－－PureLink™
实验概要The PureLink™ Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently isolate g
NCCLS的微量稀释法的PROPOTAL
National Committee for Clinical Laboratory Standards. 2003. Methods for dilution antimicrobial susceptibility tests for bacteria thatgrow aerobically,
NCCLS的微量稀释法的PROPOTAL
National Committee for Clinical Laboratory Standards. 2003. Methods for dilution antimicrobial susceptibility tests for bacteria thatgrow aerobically,
实验室装修规划设计与设备的统一
实验室装修规划设计与设备的统一实验室规划设计中涉及到的内容很多，如实验室内的设备仪器、储存、储藏家具、卫生要求、建筑和人员安全、防火要求、电路布线、给排水等等。Related to the contents of the laboratory, laboratory planning and d
PfuDNA聚合酶PCR实验方法介绍
General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec
RNAi片段siRNA设计原则
RNAi target selection rules:Targeted regions on the cDNA sequence of a targeted gene should be located 50-100 nt downstream of the start codon (ATG).S
BioRad（伯乐）Western－Blot半干法转膜的十大注意事项
首先讲转膜仪的清洗：Do not immerse the unit in liquid. Use special care when cleaning the anode plate to avoid scratching or marring the platinum. Do not use
BAC－DNA分离方法－Isolation－of－BAC－DNA－from－Largescale－Cultures
Isolation of BAC DNA from Large-scale CulturesJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid W. Russe
Example－Tissue－Bank－Protocol3
7.2 Research with de-identified tissueThis section should describe the process for supplying researchers with tissue samples that will not hav
Complete－Mouse－Necropsy
EuthanasiaEuthanasia and mouse necropsies require prior IACUC approval. The mode of euthanasia should be chosen which minimizes pain or distress to th
Acetobacter－Xylinum－Culture
OverviewGeneral guidelines on how to grow up a culture of Acetobacter xylinum, ATCC strain 53582 .Preparation of Acetobacter MediaTo prepare ∼500 ml o
The－Impact－of－Harmo...－（二）
In addition, proficiency panel projects have demonstrated that even after an experiment was done and spots were counted, considerable variation can
一步法甲醛胶连检测蛋白质相互作用
INTRODUCTIONThis protocol describes a method for chemical cross-linking of proteins using formaldehyde. With the exception of zero-length cross-linker
包涵体表达蛋白的纯化方法
Joseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid W. RussellUniversity of Texas Southwestern Medical Cen
cAMP分析－cAMP－Assays
cAMP AssaysGouzel Karimova and Daniel LadantUnite Postulante de Biochimie des Interactions Macromoleculaires, Departement de Biologie Structurale et C
亲和层析实验技术方法
INTRODUCTIONThis protocol describes a method for removing antibodies that react with bacterially encoded proteins by passing a crude preparation of im
HuanTe－Laboratory－Technology－(Hangzhou)－Co.,－Ltd.－has－entered－the－public－review－stage－for－the－ANTOP
The 2026 ANTOP Award nomination is in full swing!The "Zebrafish Lab Dedicated Equipment High-Performance Flagship Brand Award" submitted by HuanTe L
Sterile－Technique
Good sterile technique is the first and most important step in insuring consistent results when employing recombinant DNA and protein expression techn