LipoproteinAnalysisWeek2:Electrophoresis
Lipoprotein Analysis Week 2: Electrophoresis IntroductionSDS polyacrylamide gel electrophoresis (SDS PAGE) will be used to assess the purification process and to determine the apparent molecular weights of the three apoproteins.SDS-Polyacrylamide gel electrophoresisElectrophoresis is the process in which charged particles migrate through a solid or liquid matrix in response to application of an electr......阅读全文
双向电泳(twodimensional－electrophoresis,2DE)1
一、蛋白质组学概论随着人类基因组计划的实施，生命科学步入了后基因组时代，出现了不同于以往经典生物实验科学的全新的研究方式─“生物大科学”。这种生物大科学的核心思想是整体性研究，即以生物体内某类物质为对象进行完整的研究。过去对生命活动的研究仅限于研究细胞内个别的基因或蛋白质，而基因组学和蛋白质组学的目
血红蛋白电泳（hemoglobin－electrophoresis）（HbA2定量）
实验原理血红蛋白电泳（hemoglobin electrophoresis）目的是检出和确认各种正常和异常的血红蛋白。根据不同的血红蛋白带有不同的电荷，等电点不同，在一定的pH缓冲液中，血红蛋白的等电点小于缓冲液的pH时带负电荷，电泳时在电场中向阳极泳动，反之，Hb带正电荷向阴极泳动。在一定电压下，
Biosynthesis－and－Analysis－of－Bilins
The term bilin is a collective one to describe a broad group of open chain tetrapyrroles and derives from the name “bile pigments” as the first o
Analysis－of－Heme－and－Hemoproteins
Heme is perhaps the most ubiquitous cofactor found in nature and the most functionally diverse. Hemoproteins are involved in cell respiration (cy
Analysis－of－Oligosaccharide－Ligands
Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity Chromatography Analysis of Oligosaccharide Ligands by High Performance Liqui
Analysis－of－murine－BM
Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis. Techniques to do this are well established in hu
Lineage－Analysis－of－Blood
Materials:Capillary tubes1.5 mL Eppendorf microfuge tubes15 mL conical centrifuge tubes96-well V-bottom plates (Corning Costar 3894, from Fisher)Flow
CELL－CYCLE－ANALYSIS
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isol
Flow－Cytometry－Analysis
PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide r
RNA－gel－electrophoresis
MaterialsDEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37ºC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide solutions should also be
RNA－gel－electrophoresis
实验概要RNA gel electrophoresis主要试剂DEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37ºC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide sol
Pulse－Field－Electrophoresis
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fra
Agarose－Gel－Electrophoresis
实验概要Separating nucleic acid fragments by agarose gel electrophoresis.实验原理 Agarose gel electrophoresis remains the most widely used technique for sep
Agarose－gel－electrophoresis
General ProcedureCast a gelPlace it in gel box in running bufferLoad samplesRun the gelImage the gelCasting Gels0.7% agarose gel with 1kbp ladder in U
Gel－Electrophoresis－of－DNA
What is Electrophoresis?Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. In this CyberLab we
中生载脂蛋白AI与B液体双试剂性能评价
Evaluation of a Capability for The Liquid Double Reagent Assay Produced By The Zhongsheng Beikong Biotechnology and Science Inc For determine Lipo
Genomic－Southern－Blot－Analysis
This chapter describes a detailed protocol for genomic Southern blot analysis which can be used to detect transgene or endogenous gene sequences
Microtubule－Spindowns－for－Visual－Analysis
Microtubule spindowns for visual analysis can be performed on single microtubules or microtubules nucleated from axonemes/centrosomes. Although live
Protocols－for－LCM－preparation－and－analysis
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC. StainingII. Pr
Molecular－Analysis－and－Results－－DNA
Theory of CGHComparative genomic hybridization (CGH) is a fairly new molecular cytogenetic technique that allows detection of DNA sequence copy number
FACS－Analysis－of－ES－Cells
Isolate cells and dissociate to single cell suspension (can use Gibco Cell Dissociation Buffer, Accutase or Trypsin)Wash with 10% FBS/DMEM:F12For surf
Simultaneous－analysis－of－DNA－content
Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques. William Telford. Louis E. King and Pamela
PAM:－Prediction－Analysis－for－Microarrays
PAM: Prediction Analysis for MicroarraysClass Prediction and Survival Analysis for Genomic Expression Data Mining Features:Performs sample classificat
Analysis－and－Reconstitution－of－Phycobiliproteins:－Methods－for－the－...
Analysis and Reconstitution of Phycobiliproteins: Methods for the Characterization of Bilin Attachment ReactionsPhycobiliproteins are a homologous fam
CPhI－China重磅推出China－Pharma－Week活动周
—— 带您尽享制药行业盛宴！ 由欧洲博闻展览咨询有限公司(UBM EMEA)和中国医药保健品进出口商会（CCCMHPIE）主办，上海博华国际展览有限公司（UBM Sinoexpo）协办的“第十七届世界制药原料中国展”暨"第十二届世界制药机械、包装设备与材料中国展"（CPhI & P-MEC C
同行评议能走向透明吗？|－Peer－Review－Week
数据分析可以增强对科学论文的审查，但出版商必须首先同意公开此类信息。 同行评议是一件吃力不讨好的工作。整个学术界每年要花7000万个小时代表学术期刊为同行审稿。这些工作一般没有经济回报，也很少能得到应有的认可。 共享同行评议及其背后的数据有助于期刊杜绝学术出版中的造假、低效和系统性偏见。
Isolation－and－Differentiation－of－AmnionDerived－Stem－Cells－(ADSCs)
ADSC isolation, culture, and cloning Rat amnion membrane is mechanically separated from the chorion of embryonic day 18.5 Sprague–Dawley rat embry
Standard－neutral－agarose－electrophoresis
Standard neutral agarose electrophoresis Standard agarose gels can be prepared using either TBE or TAE running buffers. You will need: Either 10 x
Agarose－Gel－Electrophoresis－of－DNA
1) Dissolve 1 g of agarose in 100 ml of 1X TAE or TBE buffer (gives a 1% gel). See note for making LMP agarose gel. 2) Cast the gel with the comb in p
ELECTROPHORESIS－OF－DNA－IN－AGAROSE－GELS
ELECTROPHORESIS OF DNA IN AGAROSE GELSA). AGAROSE CONCENTRATIONS: Use 0.8% agarose (w/v) for high molecular weight DNA fragments, and 1 - 1.2% f