CloningbyLimitingDilutionofHybridoma
Author: Nanci DonackiSource: Contributed by Nanci DonackiDate Added: Tue May 14 2002Date Modified: Tue Apr 27 2004MaterialsDMEM, high glucose (Life Technologies, Inc. #10313-021 or equivalent)Fetal Bovine Serum (Life Technologies, Inc. #16000-044 or equivalent)L-glutamine (Life Technologies, Inc. #25030-149 or equivalent)Hybridoma Cloning Factor (Fisher # IG50-0615)50 ml sterile centrifuge tubes (......阅读全文
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