Raftculturemedia(akaGreen’smedia)
Raft culture media (aka Green’s media)3 parts DMEM (including glutamine or glutamax)1 part Ham’s F125% FCSvarious supplements (not everybody uses the same supplements, some don’t use any!)The following are the supplements I have used. I doubt the necessity of the last three but have not got round to trying without them.Adenine (Sigma A8626), MW=135, final concentration=180mM, working stock=45mM, Dilution f......阅读全文
Raft－culture－media－(aka－Green’s－media)
Raft culture media (aka Green’s media) 3 parts DMEM (including glutamine or glutamax) 1 part Ham’s F12 5% FCS various supplements (not everybody
Tissue－Culture－Media
We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in
Cell－Culture－Media－and－Solutions
Cell Culture Media and Solutions Dec. 18, 1990 R. Veile Antimycotic/antibiotic media: To 1 liter of sterile RPMI 1640 with 2mM L-gl
MEDIA－FOR－EMBRYO－CULTURE－AND－MANIPULATION
M16 Medium (Protocol obtained from Karen Austen-Reed from SS Tan Laboratory, Anatomy Department) For oocyte maturation and routine culture of mouse
Cell－Culture－Media－and－Solutions
Antimycotic/antibiotic media: To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add: 165.0 ml fetal bovine serum, heat inactivated 12.0 m
TISSUE－CULTURE－STOCK－SOLUTIONS－AND－MEDIA
MS MEDIUM FOR ARABIDOPSIS To 990 ml H2O add: Sucrose ........... 10.0 g MOPS .............. 0.5 g Agar .............. 8.0 g Adjust pH to
Embryonic－limb－bud－culture－in－media
Early in embryonic development, the region of the chick embryo which is determined to form a limb first differentiates from the rest of the embryo1. T
Organotypic－raft－culture－of－primary－human－keratinocytes－(PHKs)
Organotypic raft culture of primary human keratinocytes (PHKs)NotesUse 106 keratinocytes per raft.Typical ProtocolTrypsinize the PHKs off the flask an
MEDIA－AND－SOLUTIONS－REQUIRED－FOR－ROUTINE－ES－CELL－CULTURE
Media UsedTo prepare 100 ml mediumDMEM80 mlFCS15 mlNon-essential amino acids (100x)1 mlPen/strep (5,000 1U/ml, 5000 ug/ml)1 mlL-Glutamine 200 mM1 mlNu
Collagen－gel－containing－3T3－fibroblasts－dermal－equivalent－for－raft－culture
Collagen gel containing 3T3 fibroblasts (dermal equivalent for raft culture)Ingredients for 6 x collagen matrices in a 6-well plate;Roughly 3x106 J2-3
Yeast－Media
YEPD (non-selection)-1% yeast extract-2% peptone-1.5% agar (if needed for plates)After autoclaving add glucose to 2% by adding 100 ml of 20% solution
Antibiotic－Concentration－in－Media
Antibiotics should be added to lower than 60 C broth, or filter sterilized: See note. "C'' refers to chromosomal resistance: "P" plasmid based resist
Bacteria－Growth－and－Culture－Bacteria－Growth－and－Culture
Flagella and motilitymonotrichous flagella - the bacterial cell has a single flagellaperitrichous flagella - the bacterial cell has several flagella w
细胞培养——培养基和试剂
· Cell Culture Media and Solutions (Donis-Keller lab)· Cell Culture Media Formulations (LTI)Comprehensive list of cell culture media,
Bacterial－Media－Solutions－and－Stocks
3 agar (200 ml)Add 6 grams agar to 200 ml deionized water. Autoclave to sterilize.1.6 agar (200 ml)Add 3.2 grams agar to 200 ml deionized water.Autocl
Yeast－Media,－Solutions－and－Stocks
Yeast Media:Note: Synthetic complete medium can be prepared by adding media supplements (see below).Medium using 6.7 g yeast nitrogen base without ami
Transfer－of－Eukaryote－Suspension－Cultures
Materials Fibroblast suspension culture Tissue culture laminar flow hood Media appropriate to culture line used Disposable pipettes
Lactobacillus－culture
OverviewGeneral overview and guidelines on how to grow up a culture of LactobacillusMaterialsMRS broth (difco)MRS agar (difco)anaerobic conditionsProc
Retos－Buffers－－Media－Book
08-15 BufferCompoundMWFinal concentration200 mlGlycine75.07 0.1 M1.5 g1 M MgCl2 10 mM2 mlKOHto pH 9H2Oto 200 mlAAcetic Acid 1 MCompoundMWFinal concent
Culture－of－retinal－endothelial－cells－and－pericytes
Isolation of retinal microvessels1. Eyes were obtained and transported on ice.2. Eyes were cut circumferentially 3 mm posterior to the limbus, the v
细菌培养基
Preparation of LB Plate (Dr. Chastain)prepare LB plate with or without antibioticsBacterial Culture Media Recipes (WUGSC) M9 Plate Supplement (Gottsch
Tissue－Culture－Methods3
REFERENCES:R. Ian Freshney, Culture of Animal cells: A manual of basic techniques, Wiley-Liss, 1987.VI. TISSUE CULTURE PROCEDURESEach student should m
可靠的CCCadvanced－FN1无异源耗材支持人间充质干细...（三）
Results and Discussion The Eppendorf CCCadvanced™ FN1 motifs surface supports an efcient short-term expansion of hMSC-BM in various xeno-free cultu
The－Dos－and－Donts－of－Cell－Culture
Given below are a few of the essential "do’s and don'ts" of cell culture. Some of these are mandatory e.g. use of personal protective equipment (P
Dorsal－Root－Ganglion－Neuron－Culture－from－Adult－Rats
DescriptionProcedure for culturing and maintaining Dorsal Root Ganglion (DRG) neurons from adult rats. ProcedurePreparing Culture Surface- Collagen co
Freezing－and－Thawing－of－Mammalian－Cell－Lines
For long term storage of myeloma cells, hybridoma cells, T cells, and other mammalian cell lines in liquid nitrogen, and restoring them in culture.
Culture－of－Endometrial－Explants
Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion of Proteins and Prostaglandins P. J. Hansen
Maintenance－of－Cell－Culture
Maintenance of Cell Culture Author: Nanci Donacki Source: Contributed by Nanci Donacki Date Added: Tue May 14 2002
Bacterial－cell－culture
Materials Glass culture tubes with metal caps and labels Growth medium, from media room or customized Glass pipette tubes Parafilm Equ
TISSUE－CULTURE－ON－COVERSLIPS
I. Purpose:A. Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspected