IntracellularImmunofluorescentStainingforFlowCytometry2
Table 2: Human Cytokines: Intracellular Staining Quick GuideHuman Cytokines: Intracellular Staining Quick GuideHuman CytokineCell SourceActivationIncubation TimeRestimulationIntracellular BlockAntibodyIFNα2PMBCCpG A ODN2216 (20ug/ml)20 hours-Brefeldin A (added 2-4 hr post stimulation)225.CIFN-γPBMCPMA (30-50ng/ml)/Iono (1ug/ml)5hr-Brefeldin A4S.B3IL-1αPBMCLPS 100ng/ml24hr-Brefeldin A364/3B3-14IL-1β NEW!PBMCLPS 1......阅读全文
人全血Foxp3实验步骤
需要试剂Human Regulatory T Cell Whole Blood Staining Kit （#88-8996-40）包含组分：(1)、1X RBC Lysis Buffer: 200 mL (2)、Flow Cytometry Staining Buffer: 600 mL (3)、
Combined－Flow－Cytometric－Measurement－of－Two－CellSurface－Antigens2
DNA and RNA Staining6. Stain cells with 7-AAD: i. Resuspend the cells from Step 5 in 0.5 mL of NASS containing 10 µg/mL of 7-AAD. Incubatefor 20 min a
Vybrant®－DyeCycle™－Ruby－stain
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of phy
Immunodetection－of－cyclin－D1－and－D2/D3－using－flow－cytometry
DescriptionThis protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser ex
DAPI－Counterstaining－Protocols
实验概要The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove. Binding
Vybrant®－DyeCycle™－Violet－Stain
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of phy
CELL－CYCLE－ANALYSIS
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isol
CellTrace™－CFSE－Cell－Proliferation－Kit
实验概要The CellTrace™ CFSE Cell Proliferation Kit provides a versatile and well-retained cell-tracing reagent in a convenient and easy-to-use form. The
DAPI－Nucleic－Acid－Stain
实验概要The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove. Binding
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells2
Flow Cytometric AnalysisPrior to flow cytometric analysis, the vital dye Hoechst 33342 (Sigma-Aldrich, St. Louis, MO, USA) was added to cell suspensio
细胞周期的流式细胞伩检测实验方法（PI，Brdu)2
B.3. COMMENTARY B.3.1 Background information The critical steps in the methodology are cell fixation, permeabilization and the concentrations of anti-
eBioscience流式抗体常见问题与解答
1、eBioscience公司提供了哪些抗体？ A：物种——人、小鼠、大鼠、非人灵长类、犬类等。检测指标——包括细胞表面标记（CD分子，膜骨架，趋化因子受体）和胞内指标（细胞因子，核内转录因子）。抗体标记——生物素标记抗体，亲和纯化抗体以及直标荧光素抗体。eB抗体的直标荧光素有（括号内是发射光波长
Simultaneous－analysis－of－DNA－content
Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques. William Telford. Louis E. King and Pamela
Vybrant®－DyeCycle™－Green－and－Orange－Stains
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of ph
Alexa－Fluor®－488－Annexin－V/Dead－Cell－Apoptosis－Kit
实验概要Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development. Inappropriately regulated apoptosis is imp
实现蛋白marker与Annexin－V双染的同时检测的实验方法
要用AnnexinV双染法测凋亡，又想同时标记蛋白marker，甚至还有胞内指标，可是PI和7-AAD都不能洗，怎么办？ eBioscience的可固定细胞活力染料Fixable Viability Dye帮您完美解决固定、破膜以及洗涤步骤不影响Fixable Viability Dye的染色，
线粒体荧光探针大全：TMRM，Mitotracker，JC1（2）
MitoTracker Green FM ProbeMitochondria in cells stained with nanomolar concentrations of our patented MitoTracker Green FM dye (M7514) exhibit bright
Dynamic－Flow－Assay－in－a－Parallel－Plate－Flow－Chamber
Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850Flow assays allow visualization of c
免疫细胞表面抗原分子CD家族对照表(CD1CD247)
Exalpha Biologicals produces and sells products for Life Science Research, including monoclonal and polyclonal antibodies, for use in immunohistochemi
Apoptosis－Induction
IntroductionWhen studying induction of apoptosis via a cell surface molecule, it is important to first ascertain surface expression of the molecule of
Flow－Cytometric－Analysis－Of－Bcl－Family－members
DescriptionCell Fixation, staining and flow cytometric analysis ProcedureCells (106) were washed twice in FACS buffer (phosphate buffered saline PBS p
线粒体荧光探针大全：TMRM，Mitotracker，JC1（4）
Nonyl Acridine OrangeNonyl acridine orange (A1372) is well retained in the mitochondria of live HeLa cells for up to 10 days, making it a useful probe
MitoProbe™－DiIC1(5)－Mitochondrial－Membrane－Potential－Protocol
实验概要Cationic cyanine dyes have been shown to accumulate in cells in response to membrane potentialand membrane potential changes have been studied i
Protein－Staining－Procedures
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
Wholemount－staining－of－embryos
Fix embryos in formalin or MEMFA for one hour at room temperature with mixing. Rinse with TBS, replace with methanol, store at -20oC.Rehydrate by slow
Staining－Methods－for－cell
death Z. Xia 10/2/95The simplest way: trypan blue. Dead cells stain blueNon-fixed cells: FDA(fluorescein diacetate)-green, alive cells; P.I. (propidiu
Silver－Staining－Protocol
1x 40min - overnight 50% MeOH, 12% Acetic Acid1x 30min 50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde3x 20min
Alkaline－phosphatase－staining
4.5.1.1 General informationEndothelial cells possess an endogenous alkaline phosphatase (AP) activity. The enzymatic activity of AP is not restricted
Fluorescent－Staining－of－Cells
1. Fluorescent phalloidin in methanol. Phallacidin does not work as well. Dilute 10 ul 330 nM stock into 500 ul PBS for each large coverslip.2. PB
JC1分析线粒体膜电位的方法
Analysis of Mitochondrial Membrane Potentialwith the Sensitive Fluorescent Probe JC-1 Andrea Cossarizza and Stefano Salvioli Department of Biomedical