PC12CellCultureandFusion
Cell CultureMaterials1. Falcon Primaria culture dishes.2. Culture medium: DME (or F12K) with glutamine, supplemented with 7% heat-deactivated horse serum (56oC for 30 min), 7% fetal calf serum, 50 units penicillin, 50 ug/ml streptomycin, 37oC.3. ECL matrix (E-C-L cell attachment matrix, Upstate Biotech, 08-110). Stored as 250 ul aliquots in 15 ml conical tubes at -80oC. Keep it cold all the time otherwise it will for......阅读全文
植物细胞培养（plant－cell－culture）技术概述
植物细胞培养技术就是为了某种目的而在细胞水平上对离体植物细胞或原生质体进行的一系列生物工艺学操作。它包括分离、培养、再生以及一系列相关的操作。就有用化合物的生产来说，它主要是指在无菌条件下通过悬浮培养植物细胞生产有用化合物的过程。理论与技术基础：植物细胞全能性、微生物液体深层发酵系统、遗传工程
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Author: Nanci DonackiSource: Contributed by Nanci DonackiAbstract: Procedure for establishing hybridoma in one stepReagents(StemCell Technologies, Inc
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原代神经细胞培养方法－Neuron－Cell－Culture
1. Preparation of coverslips1.1- Mass cultureOur standard mass cultures are plated on astrocytes. Those, in turn, are plated on glass coverslips pre-
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PC12细胞培养
PC12细胞是大鼠肾上腺髓质嗜铬瘤分化细胞株，具神经内分泌细胞的一般特征，因其具可传代特点，广泛应用于神经生理和神经药理学研究。1. PC12细胞有两种：未分化型和分化型。其中未分化型的PC12细胞贴壁能力强，传代时需要胰酶消化，形状不规则。分化型PC12细胞传代时不需要胰酶，直接可以吹下来，形状规
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Bacteria－Growth－and－Culture－Bacteria－Growth－and－Culture
Flagella and motilitymonotrichous flagella - the bacterial cell has a single flagellaperitrichous flagella - the bacterial cell has several flagella w
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Isolation－and－growth－of－mouse－primary－myoblasts
Isolation of limb muscle from neonatal mice1. Neonatal mice by decapitation or CO2 inhalation.2. Rinse the limbs with 70% ethanol and remove them wi
体外荧光法检测核内体早期动力学
A fluorescence-based in vitro assay for investigating early endosome dynamicsSina V Barysch1,2, Reinhard Jahn1 & Silvio O Rizzoli2ABSTRACTEarly endoso
组织培养常用术语介绍（一）
随着细胞培养日益广泛地被各学科、众多的科学工作者所采用，不可避免地会在正确理解和使用各种术语上出现混乱，因此，在系统学习细胞培养之前，有必要将有关术语含义介绍如下：1.Anchorage-dependent cells or cultures （停泊或贴壁依赖性细胞或培养物）2.Apoptosis（
DNA转染
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组织培养常用术语介绍（一）
组织培养常用术语(一) 随着细胞培养日益广泛地被各学科、众多的科学工作者所采用，不可避免地会在正确理解和使用各种术语上出现混乱，因此，在系统学习细胞培养之前，有必要将有关术语含义介绍如下： 1.Anchorage-dependent cells or cultures （停泊或贴壁
细胞培养常规操作
常规操作(主要内容如下)· Aseptic Technique· Culture Vessels· Cell Counting· Primary Culture· Maintenance of Cell Line ·
PC12细胞培养方法步骤
PC12细胞是大鼠肾上腺髓质嗜铬瘤分化细胞株，具神经内分泌细胞的一般特征，因其具可传代特点，广泛应用于神经生理和神经药理学研究。1. PC12细胞有两种：未分化型和分化型。其中未分化型的PC12细胞贴壁能力强，传代时需要胰酶消化，形状不规则。分化型PC12细胞传代时不需要胰酶，直接可以吹下来，形
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GST Fusion Protein PrepItalics indicate optional steps especially useful for the analysis of untested proteins.GROWTH AND HARVESTING OF BACTERIAAdd 10
Fusion－Protein－Isolation(融合蛋白分离纯化)
Peter Novick Lab,Department of Cell Biology Yale University School of Medicinehttp://info.med.yale.edu/cellbio/Novick/Second/Protocols/Fusion.pdf1.Sta
Bacteria－Culture－Protocol
Bacteria Culture ProtocolBy 徐晓政1、TBS Medium Preparation:Prepare 1L of TBS medium contains:Tryptone 12gYeast extract 24gNaCl 5gSodium Succinate 5gGlyce
TISSUE－CULTURE－ON－COVERSLIPS
I. Purpose:A. Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspected
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AimDetection of mycoplasma by culture is the reference method of detection and has a theoretical level of detection of 1 colony-forming unit (cfu). Ho
Tissue－Culture－Media
We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in a
Acetobacter－Xylinum－Culture
OverviewGeneral guidelines on how to grow up a culture of Acetobacter xylinum, ATCC strain 53582 .Preparation of Acetobacter MediaTo prepare ∼500 ml o
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Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion of Proteins and ProstaglandinsP. J. Hansen and J.
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1. Grow cells in 5ml (or more) of LB-Amp overnight for a starter culture. 2. Grow larger culture (100x volume of starter culture) using the overnigh
Thrombin－Cleavage－of－GSTFusion－protein
INTRODUCTIONIn many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The
酵母转化
· Yeast Transformation (Gietz Lab)LiAc/SS-DNA/PEG Transformation· Yeast Transformation (Breeden Lab)LiAc method· Large-Scale Y
细胞培养（cell－culture）的一些常见问题与解答1
1、细胞传代消化时所用胰酶浓度越高越好吗？不见得！因为胰蛋白酶溶液的消化能力是和溶液的pH、温度、胰酶浓度及溶液中是否含有Ca++， Mg++离子和血清等因素有关。通常情况下，pH 8.0， 温度37 ℃其作用能力最强。另外，钙、镁离子及血清均能大大降低其消化能力。我们每次进行传代消化前，一
细胞培养（cell－culture）的一些常见问题与解答1
1、细胞传代消化时所用胰酶浓度越高越好吗？不见得！因为胰蛋白酶溶液的消化能力是和溶液的pH、温度、胰酶浓度及溶液中是否含有Ca++， Mg++离子和血清等因素有关。通常情况下，pH 8.0， 温度37 ℃其作用能力最强。另外，钙、镁离子及血清均能大大降低其消化能力。我们每次进行传代消化前，一