PreparationofLactobacillusCompetentCells
OverviewInstructions on how to prepare Lactobacillus plantarum competent cells before electrotransformation.MaterialsMRS mediaCulture of L. plantarum cellsMgCL2 (10mM)sucroseglycerolCentrifuge capable of holding four 50mL centrifuge tubes.ProcedureDay 1Prepare the following:250mL MRS media divided equally into 5 flasks (50mL each)100mL Wash buffer (10mM MgCL2)50mL Electroporation Buffer (0.5M......阅读全文
Preparation－of－Lactobacillus－Competent－Cells
OverviewInstructions on how to prepare Lactobacillus plantarum competent cells before electrotransformation.MaterialsMRS mediaCulture of L. plantarum
Competent－Cell－Preparation
实验概要Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. These cells readily incorporate f
Transforming－chemically－competent－cells
MethodThaw TSS cells on ice.Add DNA, pipette gently to mix (1μl of prepped plasmid is more than enough).Note: If you are adding small volumes (~1μl),
Preparing－chemically－competent－cells
MaterialsPlate of cells to be made competentTSS bufferLB mediaIceGlassware & EquipmentFalcon tubes500μl Eppendorf tubes, on ice200ml conical flask200μ
TOP10－chemically－competent－cells
OverviewThis protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the B
Preparation－Of－Peripheral－Blood－Cells－For－Chromosome－Analysis
实验概要Lymphocytes are differentiated cells which normally do not undergo subsequent cell divisions. By culturing lymphocytes in the presence of a mito
DNA转化
DNA转化Chemical Transformation· Transformation of Competent Cells (RbCl2 Method) (Goldberg Lab)Very nice protocol for E. Coli transformation inc
Electrotransformation－of－Lactobacillus－Spp.
OverviewGeneral guidelines for the electro-transformation of Lactobacillus sake as described by Berthier et al. and as used by Alegre et al. with Lact
96Well－Sample－Preparation－for－Adherent－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB/c 3T3, et
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
96Well－Sample－Preparation－for－Adherent－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB/c 3T3, et
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells2
Flow Cytometric AnalysisPrior to flow cytometric analysis, the vital dye Hoechst 33342 (Sigma-Aldrich, St. Louis, MO, USA) was added to cell suspensio
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells3
Moreover, when the method was applied to the analysis of the cellular composition of immature testes, the results were also in agreement with previous
Preparation－of－High－Titer－Adenovirus－in－P11－cells
adapted from Cell Biology, A Lab Manual, second edition, volume 1 -Grow up P11 cells in 15 cm plates to 70 – 80% confluence. -Infect cells with a MOI
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells1
Homogeneity of cell populations is a prerequisite for the analysis of biochemical and molecular events during male gamete differentiation. Given the c
Protocols－for－the－preparation－of－tumour－cells－for－s.c.－injections－in－mice
For the establishment of solid tumour in nude mice, tumour cells are treated in a sterile environment under a cell culturing hood. Disposable sterile
Protocols－for－the－preparation－of－tumour－cells－for－s.c.－injections－in－mice
For the establishment of solid tumour in nude mice, tumour cells are treated in a sterile environment under a cell culturing hood. Disposable sterile
酵母转化
· Yeast Transformation (Gietz Lab)LiAc/SS-DNA/PEG Transformation· Yeast Transformation (Breeden Lab)LiAc method· Large-Scale Y
Lactobacillus－culture
OverviewGeneral overview and guidelines on how to grow up a culture of LactobacillusMaterialsMRS broth (difco)MRS agar (difco)anaerobic conditionsProc
Lactobacillus－transformation
OverviewThis page details a electrotransformation protocol for Lactobacillus bacteria, specifically Lactobacillus delbruckii subsp. bulgaricus and Lac
Streptomyces:Protocols/Transformation－by－Electroporation
Description Transform E.coli cells with plasmid/cosmid DNA using the method of electrophoration (inserting plasmids into E.coli).Approx. Duration:Prep
超级感受态细胞的制备
The Inoue Method for Preparation and Transformation of Competent E. coli: "Ultra Competent" CellsJoseph SambrookPeter Maccallum Cancer Institute and T
Competent－agro－prep－for－electroporation
day 11. Start 75 mL overnight cultures of agro (strain GV3101 C58C1 Rifr pMP90 Gmr, Koncz & Schell) in YEP in 250 mL baffle flasks.2. Grow at 28 °
重组DNA的分离、克隆与测序实验手册5
C. Random fragment end-repair, size selection, and phosphorylationSince both sonicated and nebulized DNA fragments usually contain single-stranded end
重组DNA的分离、克隆与测序实验手册6
Electroporation ProtocolPreparation of Electro-competent Cells:1. Grow XL1-Blue cells on a tetracycline plate (20 ug tet/ml of LB agar)2. Inoculate 3
粘粒
Preparation of Cosmid DNAPreparation of Cosmid DNA from 50 ml Cultures (Donis Keller Lab)Cosmid vectors containing foreign DNA inserts are known to re
粘粒
Preparation of Cosmid DNAPreparation of Cosmid DNA from 50 ml Cultures (Donis Keller Lab)Cosmid vectors containing foreign DNA inserts are known to re
DNA转化实验指导1
CONTENT Transformation-Competent E. coli preparation Inoue "ultra-competent" methodRubidium chloride methodCosmid packaging protocol DNA Ligation an