ProbioticAttributesofLacticAcidBacteriaIsolates

Protocol－for－Trichloroacetic－Acid－(TCA)－Precipitation

IntroductionThe efficiency of nucleotide incorporation in DNA/RNA polymerization reactions (e.g. transcription, reverse transcription, and DNA replica

Biosynthesis－of－Cysteine－from－serine－in－bacteria－and－plants

In animals cysteine is synthesized from homocysteine, a produce of the essential amino acid methionine. In the absence of dietary methionine, animals

Maintenance－of－Probes－in－bacteria－including－Escherichia－coli

Plasmid (pUC series) containing genomic DNA fragments are maintained in E. coli strain DH5aTM. The E. coli cultures are routinely cultured at 37 C on

E.Z.N.A.®－Protocol－for－Bacteria

实验概要The E.Z.N.A. Total RNA Kit can be modified for isolation of RNA from bacterial cultures. Only cells growing at log phase should be used. Measu

Basic－procedures－for－bacteria－culture2

E. Elution of DNA fragments from agaroseDNA fragments are eluted from low-melting temperature agarose gels using an unpublished procedure first develo

Basic－procedures－for－bacteria－culture1

A. Phenol extraction of DNA samplesPhenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated

Preparation－of－Bacterial－Proteins－for－Analysis－by－2DPAGE

The following protocol has been developed for preparing soluble bacterial proteins in a form suitable for analysis by 2D-PAGE. The procedure was princ

岳田利教授团队：抗真菌乳酸菌研究领域取得新进展

　　近日，西北农林科技大学岳田利教授团队在抗真菌的乳酸菌研究领域取得新进展。研究成果以 “Recent developments in antifungal lactic acid bacteria： application， screening methods， separation， purif

Testing－the－effects－of－retinoic－acid－on－tail－formation

耐Hydrofluoric－acid电极使用技巧

1.将耐Hydrofluoric acid电极连接到ph计的输入端，正确连接。2. 正确配制耐Hydrofluoric acid电极浸泡混合液：取37.25克KCL和1.75克混合磷酸盐粉末配制成250ml混合溶液。3.严禁用耐Hydrofluoric acid电极测量能溶解PVC以及ABS的有机溶

Permeabilization－of－gramnegative－bacteria－with－KPi/hexane

Harvest (10,000 rpm for 5 min) 24 h grown (in LB pH 7.5, 37 oC, 200 rpm) Escherichia coli, Enterobacter aerogenes or Pseudomonas aeruginosa cells.Wash

Phosphoamino－acid－analysis:Mark－Kampss－method

Phosphoamino acid analysis:Mark Kamps's method1. Label your protein with 32Pi. Then subject the protein to SDS polyacrylamide gel electrophoresis

Gammaaminobutyric－Acid－Receptor－Life－Cycle

Gamma-amino butyric acid (GABA) is an inhibitory neurotransmitter that acts at synapses through binding to ligand-gated ion channels, the GABA recepto

Analysis－of－Proteins－using－Small－Format－2D－Gel－Electrophoresis

Preparation of protein samplesIntracellular virus proteinsThe following method has been developed principally for the analysis of intracellular protei

Chromosomal－DNA－Extraction－from－Grampositive－Bacteria

Chromosomal DNA Extraction from Gram-positive BacteriaThis procedure was originally developed for Listeria monocytogenes but has worked well with othe

乳酸的信息

名称：乳酸英文名：Lactic acid；2-Hydroxy propionic acid其它名称：2-羟基丙酸；α-羟基丙酸；丙醇酸构型：L型；D型；DL型CAS登录号：50-21-5（DL）；79-33-4（L）；10326-41-7（D）分子式：C3H6O3结构简式：CH3CH(OH)COOH

关于乳酸的简介

　　名称：乳酸　　英文名：Lactic acid；2-Hydroxy propionic acid　　其它名称：2-羟基丙酸；α-羟基丙酸；丙醇酸　　构型：L型；D型；DL型　　CAS登录号：50-21-5（DL）；79-33-4（L） [6] ；10326-41-7（D） [7]　　分子式：C3H

Phosphoamino－acid－analysis－of－nonTCA－precipitable－proteins

Phosphoamino acid analysis of non-TCA precipitable proteins1. Cut out gel slice, incubate 60 min with 1 ml 5.7 M HCl @ 110oC in 13 x 100 mm screw cap

Methods－for－the－Detection－of－DAminoAcid－Oxidase2

Results and DiscussionNavigationAbstractIntroductionMaterials and MethodsResults and DiscussionReferencesD-Amino-acid oxidase activity was detected in

Methods－for－the－Detection－of－DAminoAcid－Oxidase1

AbstractFour methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are de

黄酒中圆弧偶氮酸（Cyclopiazonic－acid）HPLC检测

一、背景介绍 黄酒是中国的民族特产，也称为米酒（ricewine），在世界三大酿造酒（黄酒、葡萄酒和啤酒）中占有重要的一席。酿酒技术独树一帜，成为东方酿造界的典型代表和楷模。近年来，有研究发现黄酒在发酵过程中不可避免会产生圆弧偶氮酸，这就要求必须对圆弧偶氮酸进行检验。 圆弧偶氮酸（Cyclopiaz

Gelelongation－assay－for－type－II－fatty－acid－synthesis

Gel-elongation assay for type II fatty acid synthesisSrinivas KodaliAndrew GalgociSheo Singh Dr.Jun Wang Dr., jun_wang2@merck.com, Merck Research Labo

萘啶酮酸（nalidixic－acid）（5mg/ml）的配制

溶解50mg萘啶酮酸钠盐于足量的水中，最后定容至10ml。分装成小份于-20℃贮存。常以15ug/ml的终浓度添加于生长培养基。

美国豁免乳酸的残留限量

　　据美国联邦公报消息，2019年7月26日，美国环保署发布2019-15647号条例，豁免乳酸（Lactic Acid）作为惰性成分（酸化剂）时的残留限量。　　乳酸作为酸化剂主要用于公共饮食场所、乳品加工设备、食品加工设备和器具的食品接触表面上的惰性成分。美国环保署评估了其毒理性、饮食暴露量以及对

Preparation－of－Genomic－DNA－from－Bacteria－using－Phase－Lock－GelTM

Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM (Modified from Experimental Techniques in Bacterial Genetics, Jones and Bartlet, 1990

How－to－Make－Simple－Solutions－and－Dilutions

1. Simple Dilution (Dilution Factor Method based on ratios)A simple dilution is one in which a unit volume of a liquid material of interest is combine

糖原的显示—PAS反应（periodic－acid－schiff’s－reaction）

目的要求通过PAS反应，了解糖原显示的基本原理、方法以及糖原在细胞中的分布。基本原理糖原由D-葡萄糖的分支或直链组成，在肝和肌肉最丰富。过碘酸是一种强氧化剂，能将葡萄糖中乙二醇基（CHOH-CHOH）氧化成二个游离醛基（—CHO），游离醛基与Schiff ‘s试剂反应生成紫红色产物，颜色深浅与多糖含

细菌检测

Gram Staining (+\-) (William H. Heidcamp)  Gram-Staining Procedure (MEDIC, U of Texas)Very nice and detailed method description for Gram staining  Aci

FDA批准首个可鉴别特异性致病菌检测法上市

美国食品与药物管理局（FDA）于2012年6月27日批准首个可用于鉴定引起血流感染的12种不同细菌型的核酸检测上市。该项检测被命名为Verigene GP血培养核酸检测（BC-GP），能够在首次观察到细菌增殖征象后数小时内，同时检测细菌类别和3个相关耐药基因。而传统细菌鉴定方法则需要2至4天才能获得

45种培养基配方（细菌培养基与植物培养基）－（一）

THE COMPOSITION OF MEDIA     培养基及成分     1. Acetobacter Medium (醋酸菌培养基)     Glucose (葡萄糖) 100g Yeasst extract (酵母膏) 10g     CaCO3 20g Agar (琼脂) 15g