ProbioticAttributesofLacticAcidBacteriaIsolates

Probiotic－Attributes－of－Lactic－Acid－Bacteria－Isolates

IntroductionPresently the consumption of probiotic food is highly popular worldwide for their health beneficial effects. Commonly used probiotic bacte

Lacticbacteria－Midium－Ⅱ－(乳酸菌培养基－Ⅱ)的成分和适用范围

Lactic-bacteria Midium Ⅱ (乳酸菌培养基 Ⅱ)Lacto-casein peptone (乳酪蛋白胨) 10g Beef extract (蛋白胨) 10gYeast extract (酵母膏) 5g Glucose (葡萄糖) 5gTween (吐温) 80 1g K2HP

Lacticbacteria－Medium－I－(乳酸菌培养基－I－)的成分和适用范围

Lactic-bacteria Medium I (乳酸菌培养基 I )Yeast extract (酵母膏) 7.5g Peptone (蛋白胨) 7.5gGlucose (葡萄糖) 10g KH2PO4 2gTomato juice (西红柿汁) 100ml Tween (吐温) 80 0.5m

乳酸菌的概念和种类

乳酸菌(lactic acid bacteria,LAB)是一类能利用可发酵碳水化合物产生大量乳酸的细菌的统称。这类细菌在自然界分布极为广泛,具有丰富的物种多样性,至少包含18个属,共200多种。 除极少数外,其绝大部分都是人体内必不可少的、且具有重要生理功能的菌群,广泛存在于人体的肠道中。

Bacteria－Growth－and－Culture－Bacteria－Growth－and－Culture

Flagella and motilitymonotrichous flagella - the bacterial cell has a single flagellaperitrichous flagella - the bacterial cell has several flagella w

乳酸菌和根瘤菌是细菌还是真菌

乳酸菌和根瘤菌都是细菌。乳酸菌（lactic acid bacteria，LAB）是一类能利用可发酵碳水化合物产生大量乳酸的细菌的统称。这类细菌在自然界分布极为广泛，具有丰富的物种多样性，至少包含18个属，共200多种。根瘤菌（Rhizobium）主要指与豆类作物根部共生形成根瘤并能固氮的细菌，一般

什么是乳酸菌？有什么作用？

乳酸菌(lactic acid bacteria,LAB)是一类能利用可发酵碳水化合物产生大量乳酸的细菌的通称。这类细菌在自然界分布极为广泛,具有丰富的物种多样性。它们不仅是研究分类、生化、遗传、分子生物学和基因工程的理想材料,在理论上具有重要的学术价值,而且在工业、农牧业、食品和医药等与人类生活密

Bacteria－Culture－Protocol

Bacteria Culture ProtocolBy 徐晓政1、TBS Medium Preparation:Prepare 1L of TBS medium contains:Tryptone 12gYeast extract 24gNaCl 5gSodium Succinate 5gGlyce

Biosynthesis－of－Arginine－in－Bacteria

Testing－for－Bacteria－and－Fungi

AimIn cases of gross contamination the naked eye may identify the presence of bacteria and fungi. However, it is necessary to detect low-level infecti

Biosynthesis－of－Proline－in－Bacteria

Among the twenty amino acids encoded by the genetic code, proline is unique in having a cyclic structure with its side chain connected to the amino gr

Arachidonic－Acid－Labeling

1) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum.2) Pellet cells and wash 1 time with room temperature PBS.3) Resuspend

Amino－acid－composition

There has been a recent revival of interest in the use of AA composition for the identification of proteins from 2-D gels. This technique uses the idi

The－Citric－Acid－Cycle

The Krebs cycle, also called the citric acid cycle, is a fundamental metabolic pathway involving eight enzymes essential for energy production through

Neutralizing－Arachidonic－Acid

NOTE - Use arachidonic acid from Biomol (# FA-003)1) Make up the appropriate concentration of AA in 100% ethanol.2) Add 1µl of phenol red solution.-->

研究人员在我国发现一种高毒性李斯特菌

　　据美国食品安全新闻网消息，有研究人员在我国江苏省偏远地区发现了一种新的李斯特菌病病原体。这种病原体是该细菌物种中最具毒性的代表，被确定为该地区绵羊严重疾病的病因。　　该种李斯特菌是由贾斯图斯·利比希大学吉森（JLU）的医学微生物研究所领导的国际研究小组发现的，JLU医学微生物研究所所长，德国感染

Biosynthesis－of－Chorismate－in－Bacteria－and－Plants

The biosynthesis of all three aromatic amino acids (tryptophan, tyrosine and phenylalanine) begins with the metabolic intermediate chorismate. The bio

Streaking－Bacteria－for－Single－Colonies

1. Initial inoculum:- From solid media, touch an isolated colonies with a sterile applicator or toothpick.- From liquid media, touch the culture with

Biosynthesis－of－Tryptophan－in－Bacteria－and－Plants

The aromatic amino acid tryptophan is an essential nutrient, meaning that humans and animals do not themselves have the biosynthetic machinery to synt

Principles－of－nucleic－acid－hybridization

Principles of nucleic acid hybridization5.2.1. Nucleic acid hybridization is a method for identifying closely related nucleic acid molecules within tw

Oxidation－of－Polyunsaturated－Fatty－Acid

Unsaturated fatty acids are those that contain one or more double bonds in their alkyl-chain. Polyunsaturated fats with two double bonds usually have

Preparation－of－nucleic－acid－probes

Preparation of nucleic acid probesIn standard nucleic acid hybridization assays the probe is labeled in some way. Nucleic acid probes may be made as s

DAPI－Nucleic－Acid－Stain

实验概要The  blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it  appears to associate with AT clusters in the minor groove. Binding

Agar－Plates－for－Selection－of－Clones－in－Bacteria

Cloning of PCR productsStocks:LB Agar: Luria Broth after Lennox:per LiterTryptone10 gYeast Extract5 gSodium Chloride5 gBact.  Agar15 g pH 7.0Autoclave

Biosynthesis－of－phenylalanine－and－tyrosine－in－bacteria－and－plants

Isolation－of－genomic－DNA－from－bacteria

Note: This procedure does not work well with Gram + cocci.Transfer 1.5 mL overnight culture to a 1.5 mL microfuge tube, centrifuge for 30 sec, decant

Bacteria,－cont.－Growth－and－Reproduction

Flagella and motilitymonotrichous flagella - the bacterial cell has a single flagellaperitrichous flagella - the bacterial cell has several flagella w

乳酸发酵的基本信息介绍

　　乳酸发酵 （lactic acid fermentation，fermentation of lactic acid），指糖经无氧酵解而生成乳酸的发酵过程，乙醇发酵同为生物体内二种主要的发酵形式。　　酿造生产中，大都不同程度的存在乳酸发酵过程。乳酸发酵对增进酿造调味品风味有一定帮助，酿酒中适当的

Acid－Phenol－Yeast－RNA－Prep

This is the preferred method for yeast RNA preparationuse Gloves and RNAse free solutions throughout.1. Use a YPD overnight culture to innoculate fres

核酸提取(The－Extraction－of－Nucleic－Acid)

【实验目的】 1．掌握核酸提取的基本原理和基本方法 2．掌握检测核酸浓度及纯度的原理及方法   【实验原理】 DNA是遗传信息的载体,是最重要的生物信息分子,是分子生物学研究的主要对象,因此DNA的提取也应是分子生物学实验技术中最重要、最基本的操作。如不能有效的完成DNA提取方面的工作，那就根本谈不