Hintsandprecautionsforthecare,feedingandbreedingofNeurospora2
9. Spontaneous germination. Some genotypes result in spontaneous ascospore germination (for example, the unpigmented ascospores of per-1 Type I). A significant fraction of ascospores carrying fl (fluffy) germinate spontaneously, at least in some crosses. This becomes apparent when ascospores are aged after isolating tetrads. Off-ratios may result if fl ascospores break dormancy before heatshock and are therefore kill......阅读全文
Hints-and-precautions-for-the-care,-feeding-and-breeding-of-Neurospora
Some of these notes describe what may be established routines in older laboratories, but are either unpublished or published in inaccessible places or
Hints-and-precautions-for-the-care,-feeding-and-breeding-of-Neurospora2
9. Spontaneous germination. Some genotypes result in spontaneous ascospore germination (for example, the unpigmented ascospores of per-1 Type I). A si
The-Fungal-Genetics-Stock-Center-Methods
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· Tips and hints for the storage of antibodies (Synaptic Systems)· Purification of IgG Using Protein A- or Protein G-Agarose(KPL)·