TaqManPrimerandProbeDesign
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit InstructionDesign of Probes Keep the G-C content in the 20 to 80% range.Avoid runs of an identical nucleotide. This is especially true for guanine, where runs of four or more Gs should be avoided.Do not put Gs on the 5´ end.Select the strand that gives the probe more Cs than Gs.For single-probe assays, Tm should be 68 to 70 °C when using Primer Express......阅读全文
TaqMan Primer and Probe Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit InstructionDesign of Probes Keep the G-C content in the 20 to 80% range.Avoid runs of an
PCR Primer Design（二）
Terminal Nucleotides Make a Difference Both the terminals of the primer are of vital importance for a successful amplification. The 3'-end
PCR Primer Design（一）
Molecular Biology Today 2001. 2(2): 27-32. Vinay K. Singh and Anil Kumar Bioinformatics Sub-centr
PCR Primer Design（三）
References Albert, J., and Fenyo, E.M. 1990. Simple, sensitive and specific detection of human immunodeficiency virus type 1 in clinical speci
PCR PRIMER DESIGN AND REACTION OPTIMISATION
ContentsFactors Affecting the PCR Nested Primer PCRPrimer LengthDegenerate PrimersElongation Temperature and TimeReaction BufferCycle NumberDenaturin
引物设计（Primer Design）的原则
首先引物要跟模板紧密结合，其次引物与引物之间不能有稳定的二聚体或发夹结构存在，再次引物不能在别的非目的位点引起DNA聚合反应（即错配）。围绕这几条基本原则，设计引物需要考虑诸多因素，如引物长度（primer length）、产物长度（product length）、序列Tm值（melting t
siRNAs结合生物芯片的实验设计-1
Ambion and Applied Biosystems have joined forces to provide a complete convenient, solution for performing gene silencing experiments and validating
siRNAs结合生物芯片的实验设计-2
Figure 2. Silencer ™ siRNA Validation Data Generated Using Applied Biosystems TaqMan® Gene Expression Assays. The indicated Silencer Validated siRNAs
qPCR Protocol for SNP Genotyping
实验概要Platinum® qPCR SuperMix for SNP Genotyping is a ready-to-use reaction mix for the amplification and identification of single-nucleotide polymorp
做 real-time PCR之前，你应该如何着手设计引物
正在着手做 real-time PCR 的朋友们，我想最开始肯定要考虑引物设计的问题。看完下面的文字，我保证你得到想要的引物设计方法和引物序列。第一步：拿到你所要研究的基因的序列。不要告诉我你不会找序列。如果真的不会，请参考丁香园内其他版块，如NCBI使用，序列查找等。第二步：确定你想用哪种方法。T