DNAImmunoprecipitationfortheDeterminationofDNABindingSpecificity

Andrea J. Gossett and Jason D. Lieb1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA1Corresponding author (jlieb@bio.unc.edu)INTRODUCTIONKnowledge of the DNA-binding specificity of a transcription factor aids in understanding the function of that factor in the regulation of gene transcription. One popular method ofidentifying the genomic DNA sites boun......阅读全文

DNA-Immunoprecipitation-for-the-Determination-of-DNABinding-Specificity

Andrea J. Gossett and Jason D. Lieb1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA1Corresponding autho

条带转移(Band-Shift)

Or gel mobility shift assay, gel shift assay, gel retardation, electrophoretic mobility shift assay (EMSA) EMSA Using Oligos (Mike A. Dyer)Anneal two

Immunoprecipitation

实验概要In the IP method, the protein from the cell or tissue homogenate is precipitated in an appropriate lysis buffer by means of an immune complex

Immunoprecipitation

Immunoprecipitation (IP) is one of the most widely used immunochemical techniques. Immunoprecipitation followed by SDS-PAGE and immunoblotting, is rou

Bacic-Immunoprecipitation

实验概要 Immunoprecipitation  (IP) is one of the most widely used immunochemical techniques.  Immunoprecipitation followed by SDS-PAGE and immunoblotting,

Immunoprecipitation...

实验概要Protein G, a cell wall  component produced by group G Streptococcus strains, binds the Fc part  of a wide range of immunoglobulins (Ig’s). Protein

Immunoprecipitation-Protocol

实验概要Immunoprecipitation  is a procedure by which proteins or peptides that react specifically  with an antibody are removed from solution and examined

Bradford-–-Protein-Determination

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Immunoprecipitation...-(一)

实验概要We provide a  general IP procedure including a list of reagents and a table to help  you choose the correct protein beads.Immunoprecipitation is a

Immunoprecipitation...-(二)

3. ImmunoprecipitationImmunoprecipitation can be performed using antibodies by different  methods. The use of these methods is based on the requiremen

Determination-of-IC50

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人工转录因子的部件——人类锌指结构2

Table 2: Binding sites and identity of ZFPs used in VEGF activationWe then generated artificial transcription factors by fusing the three-finger domai

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Native-chromatin-immunoprecipitation-protocol

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Coimmunoprecipitation-assays

co-IP assays can be performed between endogenous proteins or transiently or stably expressed exogenous - usually tagged - proteins. The advantage to u

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人工转录因子的部件——人类锌指结构1

Human zinc fingers as building blocks in the construction of artificial transcription factorsKwang-Hee Bae1, 4, Young Do Kwon1, 2, 4, Hyun-Chul Shin1,

Dynabeads®-CoImmunoprecipitation-Kit

实验概要The  Dynabeads® and buffers provided in this kit will enable you to a)  covalently immobilize antibodies of your choice onto the surface of  Dynab

Dynabeads®-CoImmunoprecipitation-Kit

实验概要The  Dynabeads® and buffers provided in this kit will enable you to a)  covalently immobilize antibodies of your choice onto the surface of  Dynab

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SRY基因编码的功能和结构描述

这个无内含子的基因编码一个转录因子,它是DNA结合蛋白的高迁移率族(HMG)box家族的成员这种蛋白质是睾丸决定因子(TDF),它启动男性性别决定该基因突变导致x y女性性腺发育不全(swyer综合征);含有该基因的y染色体部分移位到x染色体可导致xx男性综合征。This intronless ge

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这个无内含子的基因编码一个转录因子,它是DNA结合蛋白的高迁移率族(HMG)box家族的成员这种蛋白质是睾丸决定因子(TDF),它启动男性性别决定该基因突变导致x y女性性腺发育不全(swyer综合征);含有该基因的y染色体部分移位到x染色体可导致xx男性综合征。[由RefSeq提供,2008年7月

Fragment-Complementation-and-Coimmunoprecipitation-Assays-for-...

Plant disease resistance (R) proteins confer protection against specific pathogens or pathogen isolates. R proteins function by recognizing pathog

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Reactive oxygen species or intermediates are formed by the incomplete reduction of oxygen. Organisms living in aerobic environment generate variou