Preparationofdenaturing6％polyacrylamidegelsformicrosatelliteanalysis
Preparation of denaturing 6% polyacrylamide gels for microsatellite analysis(also for SSAP, high-resolution IRAP/ISSR and other analyses)Saadiah Jamli and Pat Heslop-Harrison November 2003University of LeicesterPreparation of the plates Bigger plate for treatement with Repel-silanea) Wear gloves. Clean the plate with laboratory detergent (type used for cleaning radioactiv......阅读全文
Lipoprotein－Analysis－Week－2:－Electrophoresis
Lipoprotein Analysis Week 2: Electrophoresis IntroductionSDS polyacrylamide gel electrophoresis (SDS PAGE) will be used to assess the purification pr
引物延伸反应
Primer extension analysis is used to determine the location and quantitate the amount of the 5´-end of specific RNAs. An end-labeled oligonucleotide i
酵母准备
Yeast DNA PreparationYeast Genomic Preparation (Gottschling Lab)Rapid method for yeast genomic DNA isolation Yeast DNA Preparation (rapid glass bead
Preparation－of－Stroma,－Thylakoid－Membrane,－and－Lumen－Fractions－from－...
Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic AnalysisFor many studies regarding
美国实验室wetern方法
WESTERN BLOT PROTOCOLIn a Western blot, proteins that are separated on polyacrylamide gels on the basis of size are transferred to a membrane for dete
Western－blotting样品准备－（二）
Sodium orthovanadate preparationAll steps to be performed in a fume hood. a. Prepare a 100 mM solution in double distilled water. b.
Amino－acid－composition
There has been a recent revival of interest in the use of AA composition for the identification of proteins from 2-D gels. This technique uses the idi
Western－blotting样品准备
实验概要Preparation of lysis buffers, protease and phosphatase inhibitors, lysate from cell culture, lysate from tissues, protein concentration, samples
Immunoprecipitation...－（一）
实验概要We provide a general IP procedure including a list of reagents and a table to help you choose the correct protein beads.Immunoprecipitation is a
NO－WEDGE－Sequencing－Gels
I have been using an alternative to wedge gels that saves acrylamide, cuts gel drying time to 20 min and gives as good or better band squashing at the
DNA－Sequencing－Gels
DNA Sequencing GelsBuffers and gel solutionsLong Ranger: we started using this in early 1995. Great stuff; the best thing is that the gels are not sti
peptide－fingerprint－mapping
Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples. Moreover, the development of two-dimension
Ingel－digestion－of－proteins－for－peptide－fingerprint－mapping
Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples. Moreover, the development of two-dimension
DNA测序
DNA测序（主要内容如下）· Sequencing Gel Preparation· Preparation of Templates · DNA Sequencing by the Dideoxy Method· DNA Sequen
Polyacrylamide－Gel－Electrophoresis－of－Oligonucleotides
1. Pour and polymerize a 20% polyacrylamide gel, no Urea.2. Remove clamps. Rinse with water. Remove comb. Rinse top of gel well.3. Insert comb teeth d
DNA指纹法
DNA Fingerprinting (David F. Betsch)the theory, procedures and applications · DNA Fingerprinting (Polyarylamide Gel) (Caltech)BAC DNA sample
ELECTROPHORESIS－OF－DNA－IN－AGAROSE－GELS
ELECTROPHORESIS OF DNA IN AGAROSE GELSA). AGAROSE CONCENTRATIONS: Use 0.8% agarose (w/v) for high molecular weight DNA fragments, and 1 - 1.2% f
基于PCR技术的染色质沉淀分析1
INTRODUCTIONAfter chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is precipitated at a locu
寡核苷酸的相关操作
In this section, you will find techniques related to oligonucleotides, such as oligo purification by acrylamide gel, annealing two oligos to make doub
SEMIDRY－ELECTROPHORETlC－TRANSFER－(WESTERN－BLOTS)
Introduction After proteins have been separated by electrophoresis, individual protein bands can often be identified by using an antibody that is
Adiponectin－Replenishment－Ameliorates－ObesityRelated－Hypertension（二）
Methods Animal and Animal Treatment KKAy male mice were purchased from Japan CLEA (Tokyo, Japan). This strain is a cross between black KK fema
Glycosphingolipid－analysis
1) Incubate cells with 1 µCi/ml of 3H-galactose for 72 hours.---> If treatment is for an extended period of time: treat in serum free media containing
Lipid－analysis
Thin layer chromatography is based on the separation of a mixture of compounds as it migrates with the help of a suitable solvent through a thin layer
Chromatin－Electrophoresis
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus CollegeExercise 10.4 - Chromatin ElectrophoresisLEVEL IIMaterials 14 M Urea6 M NaCl0.05
SSR－GEL－and－Silver－Staining－Protocol
I. EQUIPMENT:DNA sequencing unit (35 x 45 cm) & 2000V power supplyClampsLg. plastic trays (4), about 43 x 50 x 8 cm, and one lidTwo rocking platformsH
胞外基质
ECM Cell Attachment Assay (LTI)Cell Adherence Inhibition Assay (LTI)General protocol--Either monoclonal antibody or RGD peptide is added along with th
DNA－Purification－from－Agarose－Gels
1. Separate DNA fragments in an agarose gel cast with 0.5 mg/mL Ethidium bromide. Locate bands with a hand-held long-wave UV lamp.2. Slice the gel wit
Native－Acrylamide－Gels－(35－ml)
Native Acrylamide Gels (35 ml)3.5%5%6%8%30/0.8% acrylamide4.1 ml5.8 ml7 ml9.3 ml10X TBE3.5 ml3.5 ml3.5 ml3.5 mlH2027.4 ml25.7 ml24.5 ml22.2 mlDegas 1-
Agarose－Gels－for－Single－Stranded－DNA
1. Prepare 50X TAE as:242 g Tris Base57.1 mL Glacial Acetic Acid100 mL 500 mM EDTA, pH 8.0600 mL ddH2OMix. Bring volume to 1 L. Autoclave.2. Mix the f
Template－Preparation
Template PreparationThe quality of sequencing results is directlyrelated to the quality of the template. ABI recommends a minialkaline-lysis/PEG preci