Preparationofphageparticlesfromphagevectors
Pick up one phage vectors-containing colony with a sterile loop and put into 10 ml 2xTY + 10 µg/l tetracycline.Shake at 200 rpm and 37 °C untill the OD600~ 0.5.Dilute bacterial culture into 200 ml 2xTY + 10 µg/l tetracycline (dilution 1:20).Shake at 200 rpm and 30 °C approximately 20 hours.Centrifuge the bacterial culture (10000 rpm, 30 min, 4 °C) and transfer the supernatant into 50 ml Falcon Tubes. Disc......阅读全文
Preparation－of－phage－particles－from－phage－vectors
Pick up one phage vectors-containing colony with a sterile loop and put into 10 ml 2xTY + 10 µg/l tetracycline.Shake at 200 rpm and 37 °C untill the
Preparation－of－Phage－Lysates
Preparation of Phage LysatesInoculate 5 ml of lambda-broth in a glass culture tube with a single colony of an appropriate host strain of E. coli. Incu
HELPER－PHAGE－PREPARATION
HELPER PHAGE PREPARATION1. Grow an overnight of NM522 in NZCYM medium.2. Dilute overnight 1:100 and grow to an A600 = 0.3 (@2.5 x 108 cells/ml).3. Inf
噬菌体的生长
Preparing Lawn Cells for M13 Cloning (Life Technologies)Lawn cells require the F' episome for M13 infection and may be prepared Streaking Lambda
Column－Method－for－Lambda－Phage－DNA－Preparation
Purpose:Mini-prep method for lambda phage DNA purification from lysates.Time required:4 hours once the lysate is in handSpecial supplies required:BioR
M13噬菌体
· M13 Phage (Michael Blaber)Very useful background information about M13: its infection, replication, packing, cloning. If you are new to phag
Studier－Lysate－Prep
SummaryHow to make a lysate from a plaque preparation. We also use this protocol for preparation of a quick stock from previously made lysate prep.Pro
Engineering－BioBrick－vectors－from－BioBrick－parts/Colony－PCR
MaterialsPCR SuperMix High FidelityVF2 primer (5''-TGCCACCTGACGTCTAAGAA-3'')VR primer (5''-ATTACCGCCTTTGAGTGAGC-3'')De
Easy－Way－to－Clone－Genes－From－a－Phage－Library
Easy Way to Clone The protocol is oriented towards a C. albicans genomic library I made in Lambda Zap II on 7/97.The overall sequence of events is: •
cDNA－AMPLIFICATION－FROM－LAMBDAPHAGE－LIBRARY
PREPARE SOLUTIONS1. SM buffer (1 L):Mix 5.8 g of NaCl, 2 g of MgSO4-7H2O, 50 mL of 1M Tris-HCl, pH 7.5, 0.5 mL of 2% gelatin, and dH2O to 1 L (Autocla
CDNA文库
CDNA文库（主要内容如下）· Construction of cDNA Library· Construction of Genome DNA Library· Library Screening OthersConstruction of cD
Preparation－of－Mitochondria－from－Rat－Liver
Preparation of Mitochondria from Rat LiverRat liver is an ideal source for functional intact mitochondria for a number of reasons. We use Sprague-Dawl
Isolation－of－Functional－Photosystem－II－Core－Particles－From－the－Cyanoba...
This chapter contains the description of several methods used for the isolation of functional photosystem (PS)II core particles from wild-type (wt
Preparation－of－Conventional－Actin－from－Skeletal－Muscle
Modified from Spudich & Watt, 1971, JBC 246:4866.1. Mix 20 ml buffer G with each gm of muscle acetone powder. Extract with stirring on ice for 30 min.
Preparation－of－Stroma,－Thylakoid－Membrane,－and－Lumen－Fractions－from－...
Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic AnalysisFor many studies regarding
Preparation－of－cytospin－from－single－cell－suspension.
Cell number, speed and trivial details affect cytospin .Basic protocol:Prepare a cell suspension of not more than 0.5 x 106 cells / ml of protein-cont
GOLGIVESICLE－PREPARATION－FROM－PEA－HYPOCOTYLS
PREPARE SOLUTIONS1. 0.25 M Sucrose Solution:Mix 40 g of sucrose (0.25M), 50 mL of 1M KH2PO4, pH 6.65 (0.1M), 2.5 mL of 1M MgCl2 (5 mM), and dH2O to 50
A－practical－method－for－the－preparation－of－total－DNA－from－filamentous－fungi
Most methods of DNA preparation from fungi are time-consuming due to the need to first make protoplasts, expensive for chemicals such as cesium chlori
Lambda噬菌体
· Lambda DNA Preparation (Stanford DNA Sequence & Technology Center)Detailed protocol for lambda DNA preparation with recipes· Isolati
Production－of－neuronpreferential－lentiviral－vectors
实验概要Adenoviral vectors widely used to transfer foreign genes into neuronal cells possess tropism for glial cells and are toxic to infected cells.
DNA抽提
DNA抽提（主要内容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction from Cell and Tissue· Mitochondria DNA Isola
Expression－Library－Screening－(Procaryotic)－Using－APFusion－Proteins
Outline:Bacteriophage lambda is a linear double stranded DNA, approximately 50 kB in size. The two sticky ends help the phage to recircularize after e
Preparation－of－Genomic－DNA－from－Bacteria－using－Phase－Lock－GelTM
Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM (Modified from Experimental Techniques in Bacterial Genetics, Jones and Bartlet, 1990
Screening－a－cDNA－Library
Screening a cDNA Libraryfor use with HybriZAP zebrafish cDNA librariesObjectivecDNA library screening allows detection of expressed genes for subseque
Preparation－of－nucleic－acid－probes
Preparation of nucleic acid probesIn standard nucleic acid hybridization assays the probe is labeled in some way. Nucleic acid probes may be made as s
EZ－96®－M13－Isolation－Vacuum－Manifold－Protocol
实验概要The E.Z.N.A.™ family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a vari
EZ－96®－M13－Isolation－Vacuum－Manifold－Protocol
实验概要The E.Z.N.A.™ family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a vari
EZ－96®－M13－Isolation－Spin－Protocol
实验概要The E.Z.N.A.™ family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a vari
Phage－Titer
IntroductionLambda phage is a commonly used vector for transgenes. Its very high rate of infectivity makes it ideal for creating large numbers of clon
Phage－DNA
IntroductionThe phage lysate from the plate contains bacterial DNA and RNA, as well as phage DNA encased in the phage coat. The following procedure, d