Preparationofcytospinfromsinglecellsuspension.
Cell number, speed and trivial details affect cytospin .Basic protocol:Prepare a cell suspension of not more than 0.5 x 106 cells / ml of protein-containing medium. Pre-label the slides.Procedure for old type cytocentrifuge (be careful, this is not biohazard proof!!)Prepare the slides mounted with the paper pad and the cuvette in the metal holder . Load up to 200 µl of this suspension in each cuvette.&......阅读全文
Preparation－of－cytospin－from－single－cell－suspension.
Cell number, speed and trivial details affect cytospin .Basic protocol:Prepare a cell suspension of not more than 0.5 x 106 cells / ml of protein-cont
组织学——组织制备
· Histological techniques (William H. Heidcamp)Very detailed guide to histological techniques, like fixation, dehydration, embedment and subs
Preparation－of－Mitochondria－from－Rat－Liver
Preparation of Mitochondria from Rat LiverRat liver is an ideal source for functional intact mitochondria for a number of reasons. We use Sprague-Dawl
Preparation－of－phage－particles－from－phage－vectors
Pick up one phage vectors-containing colony with a sterile loop and put into 10 ml 2xTY + 10 µg/l tetracycline.Shake at 200 rpm and 37 °C untill the
Preparation－of－Conventional－Actin－from－Skeletal－Muscle
Modified from Spudich & Watt, 1971, JBC 246:4866.1. Mix 20 ml buffer G with each gm of muscle acetone powder. Extract with stirring on ice for 30 min.
Preparation－of－Stroma,－Thylakoid－Membrane,－and－Lumen－Fractions－from－...
Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic AnalysisFor many studies regarding
GOLGIVESICLE－PREPARATION－FROM－PEA－HYPOCOTYLS
PREPARE SOLUTIONS1. 0.25 M Sucrose Solution:Mix 40 g of sucrose (0.25M), 50 mL of 1M KH2PO4, pH 6.65 (0.1M), 2.5 mL of 1M MgCl2 (5 mM), and dH2O to 50
CELL－MEMBRANE－PREPARATION
I. Solutions: A. Ca and Mg free Phosphate Buffered Saline (PBS) solution, buffered with 0.02M Hepes. pH=7.4 B. Ca and Mg free PBS, buffered with
Competent－Cell－Preparation
实验概要Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. These cells readily incorporate f
A－practical－method－for－the－preparation－of－total－DNA－from－filamentous－fungi
Most methods of DNA preparation from fungi are time-consuming due to the need to first make protoplasts, expensive for chemicals such as cesium chlori
BrdU－Labeling－Protocol
实验概要The thymidine analog, 5-bromo-2-deoxyuridine (BrdU),is a common reagent used for cell proliferation assays and for the detection of apoptotic
SingleCell－Electroporation－in－Xenopus
Single-Cell Electroporation in XenopusXue Feng Liu and Kurt HaasINTRODUCTIONSingle-cell electroporation (SCE) is a versatile technique for delivering
Heterogeneity－of－SingleCell－Gene－Expression－Across－Phenotypically（一）
Introduction Multi-cellular populations are fundamentally driven by the collective properties of individual cells. However, our understanding of ge
Preparation－of－Rat－Liver－Cell－Cytosol
These protocols should yield enough cytosol and organelles for 1-200 MT/Organelle motility assays.Solutions and Reagents Freshly removed or flash fro
Preparation－of－Genomic－DNA－from－Bacteria－using－Phase－Lock－GelTM
Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM (Modified from Experimental Techniques in Bacterial Genetics, Jones and Bartlet, 1990
Cosmid－Cloning:－Cell－preparation,－DNA－packaging,－and－Cell－Transfection
Cosmid Cloning: Cell preparation, DNA packaging, and Cell TransfectionProtocol taken from Stratagene's Gigapack packaging extracts instruction man
Method:－Reactivating－Cell－Lines－and－Cell－Growth－for－DNA－Preparation
Purpose:Cell lines are reactivated and grown to a count of 1 x 108 cells. The cells are pelleted and stored frozen at -80 degrees C prior to DNA extra
RNA－FISH－on－cultured－cells－in－interphase
IntroductionFluorescence in situ hybridization (FISH) has become a widely used method in genome and molecular genetic studies. The technique is highly
Method:－Preparation－of－Lymphocyte－Cell－Pellet－for－Storage
Method: Preparation of Lymphocyte Cell Pellet for StorageJune 10, 1990Rosalie VeilePurpose:Following propagation to 1 X 108 cells, lymphoblastoid cell
Human－Peripheral－Blood－Mononuclear－Cell－Preparation
This protocol describes a procedure for isolating human peripheral blood mononuclear cells (lymphocytes and monocytes) from a Buffy Coat (obtained fro
细胞遗传学——原位杂交(ISH)
In Situ Hybridization· In Situ Hybridization (jsmith1@po-box.mcgill.ca)In situ hybridization, as the name suggests, is a method of localizing,
Detection－of－MicroRNA－Heterogeneity－in－Single－Cells－Using－an－Automated
Introduction MicroRNA (miRNAs) are short (18–24 nucleotides), non-coding RNAs that regulate gene expression by both disrupting messenger RNA (mRNA
Studier－Lysate－Prep
SummaryHow to make a lysate from a plaque preparation. We also use this protocol for preparation of a quick stock from previously made lysate prep.Pro
Single－Cell－ICPMS－应用研究
癌症研究 • 环境毒理学 • 生物技术和细胞科学 • 药物设计 • 四组四极杆： · 第yi组 四极杆离子偏转器（Q0，Quadrupole Ion Deflector）是一个基于离子能量的静电质量分析器，对离子进行动态聚焦和质量筛选，同时把离子偏转90度
Single－Cell－PCR－单细胞PCR实验技术
Single Cell PCR(Protocol provided by Carolyn Troeger)Cell picking c Axiovert 100/Zeiss, extended glass capillary/Drummond, Broomall and a micromanipul
Single－Cell－ICPMS－应用研究
• 癌症研究• 环境毒理学• 生物技术和细胞科学• 药物设计• 四组四极杆：· 第yi组四极杆离子偏转器（Q0，Quadrupole Ion Deflector）是一个基于离子能量的静电质量分析器，对离子进行动态聚焦和质量筛选，同时把离子偏转90度以实现与中性成分和光子分离，导入下一级四极杆· 第二
转基因
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline (University of Michigan Transgenic Animal Model Core)Thi
Heterogeneity－of－SingleCell－Gene－Expression－Across－Phenotypically（二）
Figure 2: Verification of C1 Single-Cell mRNA-Seq data quality. a)ERCC RNA Spike-In Control Mix 1 was applied to a C1 IFC at a total transcript in
RNA－Isolation－From－Animal－tissue－or－cell－culture
实验概要This method is designed for most animal tissues and culture cells. For RNA isolation from fibrous tissue, follow the specialized protocol on pag
Double－immunofluore...
实验概要We provide a protocol for immunofluoresent double staining incubating the antibodies together.In order to be able to examine the co-distributi