LambdaPhageDNAQuickprep
suspend a single plaque in 1 ml PSBadsorb 10 min at 37°C: 0.1 ml eluted phage*/0.1 ml MgCa/0.1 ml saturated K802 culture grown in NZY broth/0.2% maltoseuse 10 ml of this to inoculate 2.5 ml NZY, shake ovn at 37°C or until lysedcool to RT, then add 2 drops of CHCl3add 5 ml of 10 mg/ml DNase I (20 mg/ml final) and gently shake for 25 mindivide lysate into two Eppendorf tubes, spin 5 mintransfer 0.6 ml of each tube into......阅读全文
Lambda－Phage－DNA－Quickprep
suspend a single plaque in 1 ml PSBadsorb 10 min at 37°C: 0.1 ml eluted phage*/0.1 ml MgCa/0.1 ml saturated K802 culture grown in NZY broth/0.2% malto
Column－Method－for－Lambda－Phage－DNA－Preparation
Purpose:Mini-prep method for lambda phage DNA purification from lysates.Time required:4 hours once the lysate is in handSpecial supplies required:BioR
Lambda噬菌体
· Lambda DNA Preparation (Stanford DNA Sequence & Technology Center)Detailed protocol for lambda DNA preparation with recipes· Isolati
cDNA－AMPLIFICATION－FROM－LAMBDAPHAGE－LIBRARY
PREPARE SOLUTIONS1. SM buffer (1 L):Mix 5.8 g of NaCl, 2 g of MgSO4-7H2O, 50 mL of 1M Tris-HCl, pH 7.5, 0.5 mL of 2% gelatin, and dH2O to 1 L (Autocla
Phage－DNA
IntroductionThe phage lysate from the plate contains bacterial DNA and RNA, as well as phage DNA encased in the phage coat. The following procedure, d
Preparing－Lambda－DNA
Preparing Lambda DNA1- Coliphage lambda DNA is a widely used vector for recombinant DNA. The middle third of its 48,000 bp contains no genes required
Lambda－DNA－Preparation
Lambda DNA PreparationThis is a plate method that gives very good yield for cloning. I have combined the Promega and Maniatis protocols.Solutions T-TY
Lambda（噬菌体）DNA－Miniprep
David HarryInstitute of Forest GeneticsUSDA Forest ServicePacific Southwest Research StationAugust 26, 1993Background :There are many published method
Packagene®－Lambda－DNA－Packaging－System
Packagene® Lambda DNA Packaging SystemThe Packagene® Lambda DNA Packaging System is derived from the unique one-strain host system (Rosenberg). The Sy
噬菌体的生长
Preparing Lawn Cells for M13 Cloning (Life Technologies)Lawn cells require the F' episome for M13 infection and may be prepared Streaking Lambda
CDNA文库
CDNA文库（主要内容如下）· Construction of cDNA Library· Construction of Genome DNA Library· Library Screening OthersConstruction of cD
Preparation－of－phage－particles－from－phage－vectors
Pick up one phage vectors-containing colony with a sterile loop and put into 10 ml 2xTY + 10 µg/l tetracycline.Shake at 200 rpm and 37 °C untill the
Phage－Titer
IntroductionLambda phage is a commonly used vector for transgenes. Its very high rate of infectivity makes it ideal for creating large numbers of clon
核酸的修饰酶
The restriction/modification system in bacteria is a small-scale immune systemfor protection from infection by foreign DNA. W. Arber and S. Linn (1969
HELPER－PHAGE－PREPARATION
HELPER PHAGE PREPARATION1. Grow an overnight of NM522 in NZCYM medium.2. Dilute overnight 1:100 and grow to an A600 = 0.3 (@2.5 x 108 cells/ml).3. Inf
Preparation－of－Phage－Lysates
Preparation of Phage LysatesInoculate 5 ml of lambda-broth in a glass culture tube with a single colony of an appropriate host strain of E. coli. Incu
烈性噬菌体（virulent－phage）和温和噬菌体（temperate－phage）
噬菌体(bacteriaphage or phage)是病毒的一类，结构很简单，基本上由一个蛋白质外壳包裹着一些核酸组成的。噬菌体的多样性来自于组成其外壳的蛋白质的种类，以及其染色体的类型和结构的不同。（一）烈性噬菌体（ virulent phage）遗传学上应用最广泛的烈性噬菌体是大肠杆菌（ E.
Easy－Way－to－Clone－Genes－From－a－Phage－Library
Easy Way to Clone The protocol is oriented towards a C. albicans genomic library I made in Lambda Zap II on 7/97.The overall sequence of events is: •
酵母人工染色体
· Easy YAC Preparation Method (Andrew Davies,Shaw lab)· Screening YAC libraries (Donis Keller Lab)This is a method for screening YAC l
Isolation－and－Quantification－of－Genomic－DNA－from－Mycobacterium－tuberculosis
Part A. Isolation of Nucleic AcidsNOTE: CAUTION! STEPS 1-10 SHOULD BE PERFORMED USING APPROPRIATE PROCEDURES FOR HANDLING MATERIAL POTENTIALLY CONTAMI
如何借助epMotion－5073l移液工作站完成微量体积的...（一）
如何借助epMotion 5073l移液工作站完成微量体积的qPCR反应体系构建在进行微量体系的液体操作时，如何避免人为误差与日间变化，如何保证结果的一致性提高实验效率？如果减少冗长枯燥的重复操作，减少昂贵试剂的使用，提升操作体验节省实验经费？本篇应用旨在介绍如何用10 μL分液工具实现全自动的微量
DNA抽提
DNA抽提（主要内容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction from Cell and Tissue· Mitochondria DNA Isola
Expression－Library－Screening－(Procaryotic)－Using－APFusion－Proteins
Outline:Bacteriophage lambda is a linear double stranded DNA, approximately 50 kB in size. The two sticky ends help the phage to recircularize after e
Genomic－Libraries
Genomic DNA libraries Size of some genomes and chromosomes:Comparative Sequence Sizes(Bases)(yeast chromosome 3)350 ThousandEscherichia coli (bacteriu
Standard－Operating－Procedures－for－T1Phage－Testing－Assay
I. Introduction:This assay uses a lawn of phage-susceptible E. coli (DH10B) embedded in a layer of agarose. This top agarose lays on a bed of standard
DNA的酶学操作
DNA的酶学操作DNA Modifying Enzymes (Michael Blaber)Introduction to bacterial restriction/modification system. It provides very useful background knowledge
Differential－cDNA－Screening－Procedures
Differential cDNA Screening ProceduresThe protocols listed refer to cDNA library construction and preliminary differential screening procedures. They
重组DNA的分离、克隆与测序实验手册3
G. Bacterial cell maintenanceFour strains of E. coli are used in these studies: JM101 for M13 infection and isolation (4), XL1BMRF' (Stratagene) f
M13噬菌体
· M13 Phage (Michael Blaber)Very useful background information about M13: its infection, replication, packing, cloning. If you are new to phag
DNA－Molecular－Weight－Markers
DNA Molecular Weight Markers Lambda DNA Hind III DigestFragmentBase Pairs123,31029,41536,55744,36152,32262,02775648125Lambda DNA EcoR I DigestFragment