Sectioningstainedembryos.
The protocols for plastic and wax sections as used by the Vize lab. These protocols work, but they have not been optimized. If anyone has better protocols please let us know.Samples.Stain samples strongly, remember that in a section only the very strongest stain will be visible, even quite strong background will not be visible. BCIP/NBT is the only stain we have successfully used, we have tried Red new fuschin but it......阅读全文
连续断层3D重构中的超薄切片制备
当需要以纳米级分辨率观察样品超微结构时，科学家通常借助电子显微镜（观察表面结构的扫描电镜 SEM 和观察内部结构的透射电镜 TEM）——它们是当前科研界可使用的最大显微成像工具。电镜成像要求对样品进行通常 20-150 nm的超薄切片，使用超薄切片机切割的切片厚度薄、表面平整、光滑且无人为干扰因
Protocols－for－LCM－preparation－and－analysis
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC. StainingII. Pr
siRNAs结合生物芯片的实验设计2
Figure 2. Silencer ™ siRNA Validation Data Generated Using Applied Biosystems TaqMan® Gene Expression Assays. The indicated Silencer Validated siRNAs
Largescale－Immunocytology
This protocol describes our method for preparing cells for immunofluorescence, in which all incubations and washes are performed in microtiter dishes.
Fast－and－reliable－miniprep－RNA－extraction－from－Neurospora－crassa
We have developed a method for isolating high quality total RNA from N. crassa mycelia that reliably yields large quantities. It is possible to extrac
DNA－EXTRACTION－FROM－MICRODISSECTED－PARAFFIN－SECTIONS
This is a four day procedure so it's best to start on Monday or Tuesday.CASE SELECTION:H&E stained thin sections are first reviewed by a pathologi
A－simple,－rapid－procedure－for－the－isolation－of－DNA－for－PCR
N.M. DuTeau and J.F. Leslie - Department of Plant Pathology, Throckmorton Hall, Kansas State University, Manhattan, KS 66506-5502The polymerase chain
QUALITATIVE－ANALYSIS－OF－DNA－FRAGMENTATION－BY－AGAROSE－GEL－ELECTROPHORESIS
1. IntroductionNuclear morphology changes characteristic of apoptosis appear within the cell together with a distinctive biochemical event: the endonu
纳米流式分析法肾结石高通量无创检测新方法
肾结石是泌尿外科最常见的的疾病之一，是一种全球性疾病。中国是世界上肾结石患者最多的国家之一。近年来，肾结石在我国的发病率有逐渐上升趋势，大约5%-15%的人群一生中会罹患肾结石。肾结石严重影响患者的身体健康，如果肾结石得不到及时诊断和治疗,可能会造成尿路阻塞,排尿疼痛和肾积水，严重者甚至可能造成尿毒
光片成像模块升级共聚焦显微镜：成像更快速光毒性更低
对生物样品进行快速可靠的原位成像以揭示与复杂的多细胞生物相关的动态过程一直都是光学成像的一大目标。传统的激光共聚焦显微镜虽然具有优异的3D荧光成像功能，提供了非常高的空间分辨率，但是在某些实验中，成像速度不够快和光漂白问题依然不容忽视。光片技术的提出就很好地解决了这些问题，同时还保有优异的空间分辨率
LIVE/DEAD®－Violet－Viability/Vitality－Kit
实验概要The LIVE/DEAD® Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the simultaneo
Assay－of－superoxide－dismutase－activity2
Cuvette holders in the sample chamber of the spectrophotometer were thermo-controlled at 25°C. For the blank test, 100 ml of 50 mM potassium phosphate
DNA的酶学操作
DNA的酶学操作DNA Modifying Enzymes (Michael Blaber)Introduction to bacterial restriction/modification system. It provides very useful background knowledge
流式细胞仪技术专辑
Flow Cytometry Analysis (Springer Lab, Harvard University) Flow cytometry employs instrumentation that scans single cells flowing past excitation sour
阿尔茨海默症模型小鼠全脑Aβ斑块及其周围结构的全景图
在全脑范围同时获取多种结构的高分辨图谱，对于剖析脑功能及阿尔茨海默症（AD）等神经系统疾病的发病机制具有重要意义。当前，已有的成像技术和方法在实现大尺度、高分辨率的多种脑结构元素同步成像方面，面临挑战。近日，中国科学院上海药物研究所MOST与图像融合技术服务部在Frontiers in Neuros
神奇！Science重要发现：母爱竟会改变孩子DNA
在这篇题为“Early life experience drives structural variation of neural genomes in mice”的论文中，来自Salk研究所的科学家们证实，雌性小鼠的育儿方式真的能改变后代的DNA。这项成果支持了先前关于“儿童环境如何影响人类大
Observation－of－living－and－plasticembedded－chick－embryos
The development of chick embryos has been studied since Aristotle. It is one of the most intensely studied organisms. One reason for this is that ther
Column－Method－for－Lambda－Phage－DNA－Preparation
Purpose:Mini-prep method for lambda phage DNA purification from lysates.Time required:4 hours once the lysate is in handSpecial supplies required:BioR
玉米Proline－responding－1(pro1)突变在蛋白合成和细胞...（二）
Pro1基因编码一个△1-吡咯啉-5-羧酸合成酶(P5CS) 对玉米经典突变pro1基因进行图位克隆发现，玉米Pro1基因编码一个△1-吡咯啉-5-羧酸合成酶(P5CS) (Figure 2A)，它是催化以谷氨酸为前体合成脯氨酸过程中的限速酶(Figure 2B, C, D, E)。Pro1基因功能
Standard－CellTrace™－Violet－TCell－Procedure
实验概要The CellTrace™ Violet Cell Proliferation Kit provides a versatile and well-retained cell tracing reagent in a convenient and easy-to-use form. T
DNA电泳
DNA电泳（主要内容如下） Preparation of Agarose Gel and Electrophoresis Extraction of DNA From Agarose Gel Extraction of DNA from Acrylamide Gels DNA Marker
流式细胞仪技术专辑
最方便的实验干货查询工具微信扫码进入「丁香实验」小程序编辑： 呜咽分享到： Flow Cytometry Analysis (Springer Lab, Harvard University)Flow cytometry employs instrumentation that scan
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Dark Field ViewingPrincipleTo view a specimen in dark field, an opaque disc is placed underneath the condenser lens, so that only light that is scatte
Identification－of－a－Mutant－Kinase/ATP－Analog－Pair1
Identification of a Mutant Kinase/ATP Analog PairScott T. Eblen, N. Vinay Kumar, and Michael J. WeberDepartment of Microbiology and Cancer Center, Uni
Denaturing－Agarose－Gel－Electrophoresis－of－RNA
The overall quality of an RNA preparation may be assessed by electrophoresis on a denaturing agarose gel; this will also give some information about R
Cloning－of－small－RNAs－with－5’－phosphate－and－3’－OH－ends01
IntroductionThe following protocol describes a procedure for the purification and cloning of miRNAs and other small RNAs in the 20-30 nucleotide size
Bromodeoxyuridine－Immunohistochemistry
Introduction: This method for the detection of cellular proliferation includes several modifications of a previously published protocol (Hayashi, et a
AD模型小鼠全脑Aβ斑块及其周围多种结构的高精度全景图
在全脑范围同时获取多种结构的高分辨图谱，对于剖析脑功能及阿尔茨海默症（AD）等神经系统疾病的发病机制具有重要意义。当前，已有的成像技术和方法在实现大尺度、高分辨率的多种脑结构元素同步成像方面，面临挑战。 近日，中国科学院上海药物研究所MOST与图像融合技术服务部在Frontiers in Ne
ImmunohistochemistyEnzymatic－Protocol
OverviewR&D Systems provides monoclonal, polyclonal and biotinylated antibodies for immunohistochemical use. The following protocol has been developed
UV－CrossLinking－an...－（二）
实验步骤1. UV cross-linking of tissue culture cells 1) Remove media and add ice-cold PBS to cells (e.g. use cells grown in a 10 cm plate for three ex