SpecimenPreparationforScanningElectronMicroscopy
Specimen Preparation for Scanning Electron MicroscopyWe recommend consultation with one of the lab directors before preparing specimens. The methods presented here provide an overview of preparation techniques for a variety of specimens.Conductive Specimens (such as metallic objects):Usually, these specimens do not have to be sputter coated. Simply mount the specimen on a SEM stub using conductive paint or putty......阅读全文
Light－Microscopy
The light microscope, so called because it employs visible light to detect small objects, is probably the most well-known and well-used research tool
DAPI－Nucleic－Acid－Stain
实验概要The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove. Binding
如何通过透射电子显微镜（TEM）初步确定待测样品
束1. 选区电子衍射（selected area electron diffraction）： 判断样品的晶体结构，晶格常数，点阵应变等。通常需要结合X射线衍射来综合判断。但选区电子衍射的选区范围最小只能达到~200 nm。这主要是来自物镜像差的限制。现在前沿的技术是纳米电子微区衍射(Nanobea
Histochemistry－－Introduction
A few cell types are thin enough to be viewed directly in a microscope (algae, protozoa, blood, tissue cultures), but most tissues (kidney, liver, bra
Microscopes－in－Cell－Biology
Microscopes in Cell BiologyIntroductionMicroscopy has a major role in the study of cells. From the very beginning, researchers have tried to develop w
单细胞藻吸收Cu2+,Zn2+,Se4+,Cd2+的扫描电镜X射线能谱分析
This paper determined the lethal concentration （LC） and hemi-inhibition concentration （HIC） of inorganic element Cu2+, Zn2+,Se4+,Cd2 to the unicellula
透射电子显微镜是如何确定待测样品属于某种物质的？
在透射电子显微镜下，可以通过以下几种方式综合判断样品：1. 选区电子衍射（selected area electron diffraction）： 判断样品的晶体结构，晶格常数，点阵应变等。通常需要结合X射线衍射来综合判断。但选区电子衍射的选区范围zui小只能达到~200 nm。这主要是来自物镜像差
透射电子显微镜是如何确定待测样品属于某种物质的？
在透射电子显微镜下，可以通过以下几种方式综合判断样品：1. 选区电子衍射（selected area electron diffraction）： 判断样品的晶体结构，晶格常数，点阵应变等。通常需要结合X射线衍射来综合判断。但选区电子衍射的选区范围zui小只能达到~200 nm。这主要是来自物镜像差
透射电子显微镜是如何确定待测样品属于某种物质的？
在透射电子显微镜下，可以通过以下几种方式综合判断样品：1. 选区电子衍射（selected area electron diffraction）： 判断样品的晶体结构，晶格常数，点阵应变等。通常需要结合X射线衍射来综合判断。但选区电子衍射的选区范围zui小只能达到~200 nm。这主要是来自物镜像差
Immunofluorescence－Microscopy－Protocol
实验概要 Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically whic
Immunofluorescence－Microscopy－Protocol
实验概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which i
Phase－Contrast－Microscopy
Phase Contrast MicroscopyPrincipleMost of the detail of living cells is undetectable in bright field microscopy because there is too little contrast b
细胞组分和细胞器——细胞骨架
Fixation and Immunofluorescence of the Cytoskeleton (Mitchison Lab) Recycling Tubulin (Mitchison Lab) Labeling Tubulin and Quantifying Labeling Stoi
生命科学常用实验仪器名称中英文对照表（二）
核磁共振波谱仪 Nuclear Magnetic Resonance SpectrometerNMR电子顺磁共振波谱仪 Electron Paramagnetic Resonance SpectrometerESR极谱仪 Polarograph伏安仪 Voltammerter自动滴定仪 Automa
常用实验仪器名称中英文对照表（一）
仪器中文名称 仪器英文名称(缩写) 原子发射光谱仪 Atomic Emission Spectrometer(AES) 电感偶合等离子体发射光谱仪 Inductive Coupled Plasma Emission Spectrometer(ICP) 直流等离子体发射光谱仪 Dir
扫描电子显微镜样品制备技术
扫描电子显微镜样品制备技术(preparationof specimens for scanning electron microscopy):扫描电子显微镜以观察样品的表面形态为主，因此扫描电子显微镜样品的制备，必须满足以下要求:①保持完好的组织和细胞形态;③充分暴露要观察的部位;③良好的导电性和
Analysis－of－murine－BM:－specimen－choice,－sampling－and－processing.
Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis. Techniques to do this are well established in hu
Immunofluorescence－/－Confocal－Microscopy－Protocol
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
Immunofluorescence－/－Confocal－Microscopy－Protocol
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
Transmission－Electron－Microscope－(TEM)
所谓TEM，就是一个放大镜叠加了一台照相机。这台放大镜的放大倍数比较高，可高达一百万倍。当然，抛开分辨率谈放大倍数都是耍流氓，那么，TEM的分辨率有多高呢？答案是 it depends。一般来说，TEM的分辨率要在1到2个纳米，STEM更高，但是STEM得成像技术类似于SEM，但用的不是二次电子。我
Preparation－of－tubulin
Although many protocols for tubulin preparation are available, the procedure described below is the simplest and highest yielding preparation I have
SMEAR－PREPARATION
The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria
Platelet－Preparation
OUTLINEIn order to avoid platelet activation all manipulations must be performed as quickly and as acurate as possible.Work on ice if possible! This p
Template－Preparation
Template PreparationThe quality of sequencing results is directlyrelated to the quality of the template. ABI recommends a minialkaline-lysis/PEG preci
Liposome－Preparation
Liposome PreparationOBJECTIVE:Method for incorporating proteins into liposomes for liposome swelling assay or as an alternative antigen presentation m
CAM－preparation
8 eggs per day, day 7- day 13 cut CAM, wash in precooled PBS,in 10 ml WASH 1 (PBS, 5 mM EDTA, COMPLETE) on icecut in pieces in petri dish on icecentri
扫描电镜透射模式（STEM）
扫描电子显微镜已成为表征物质微观结构不可或缺的仪器。在扫描电镜中，电子束与试样的物质发生相互作用，可产生二次电子、特征X射线、背散射电子等多种的信号，通过采集二次电子、背散射电子得到有关物质表面微观形貌的信息，背散射电子衍射花样得到晶体结构信息，特征X-射线得到物质化学成分的信息，这些得到的都是接近
Silver:－TimeLapse－Microscopy
Pad Preparation 1. Microwave 2% agarose (mix of low-melt and normal, to taste) in Thorn media (see below). (If you have used different percentages o
Immunofluorescence－Microscopy－of－tissue－culture－cells
Immunofluorescence Microscopy of tissue culture cellsThese methods are written for direct staining of filamentous actin with bodipy FL-phallicidin and
MetalEnhanced－Immu...－（二）
实验步骤1. Preparation of Fluorescein-Doped PVA FilmsThe 0.5% PVA solution containing disodiumfluorescein was spin-coated on slide substrates at 3,000 rp