TetrahymenaFixationforTransmissionElectronMicroscopy
Tetrahymena Fixation for Transmission Electron MicroscopyPellet Tetrahymena cells in a clinical centrifuge.OPTIONAL: Suspend cells in HNMK (50 mM HEPES, pH 6.9, 36 mM NaCl, 0.1 mM Mg acetate, 1 mM KCl ) for 10-20 min at room temperture. This will remove precipitates present in the proteose peptone and provide cleaner surfaces if cells are processed for SEM.Pour off most of the medium and suspend cells in a slurr......阅读全文
Tetrahymena－Fixation－for－Transmission－Electron－Microscopy
Tetrahymena Fixation for Transmission Electron Microscopy Pellet Tetrahymena cells in a clinical centrifuge. OPTIONAL: Suspend cells in HNMK (50 m
Chlamydomonas－Fixation－for－Transmission－Electron－Microscopy
Chlamydomonas Fixation for Transmission Electron Microscopy Solutions: Chlamydomonas culture medium + 2% glutaraldehyde (5 ml medium + 0.9 ml 25% g
Generic－Fixation－for－Electron－Microscopy
Generic Fixation for Electron Microscopy The best way to fix a sample for electron microscopy is to follow a procedure developed and proven by other
Use－of－Transmission－Electron－Microscopy
Use of Transmission Electron Microscopy Overview A protocol describing the use of Zeiss EM9-S transmission electron microsco
Fixation－and－Embedding－of－Microtubules－for－Electron－Microscopy
(This procedure can also be used for virtually any material that must be pelleted prior to fixation and thin sectioning) Primary fix: 2% glutaralde
EPON－resin－mixture－for－transmission－electron－microscopy
EPON resin mixture for transmission electron microscopyFor Epon WPE 153:~120 ml~60 ml~30 mlMix A:Embed 81244 ml22.1 ml11.1 mlDDSA67 ml33.3 ml16.7 mlMi
显微镜技术——电子显微技术
The Transmission Electron Microscope (TEM) (HEI)An explanation of how the TEM works. TEM Specimen Preparation (HEI) Serial Sectioning (Walter Steffe
ELECTRON－MICROSCOPY
E.M. PROCESSING SCHEDULE - EPOXY RESINFix tissue in 2.5% glutaraldehyde in 0.1M sodium cacodylate buffer at 4oC, for a minimum of 4 hours. Tissue shou
透射电子显微镜(Transmission－electron－microscopy,－TEM)
透射电镜具有很高的空间分辩能力，特别适合纳米粉体材料的分析。其特点是样品使用量少，不仅可以获得样品的形貌，颗粒大小，分布以还可以获得特定区域的元素组成及物相结构信息。透射电镜比较适合纳米粉体样品的形貌分析，但颗粒大小应小于300nm，否则电子束就不能透过了。对块体样品的分析，透射电镜一般需要对样品进
Transmission－Electron－Microscope－(TEM)
所谓TEM，就是一个放大镜叠加了一台照相机。这台放大镜的放大倍数比较高，可高达一百万倍。当然，抛开分辨率谈放大倍数都是耍流氓，那么，TEM的分辨率有多高呢？答案是 it depends。一般来说，TEM的分辨率要在1到2个纳米，STEM更高，但是STEM得成像技术类似于SEM，但用的不是二次电子。我
Specimen－Preparation－for－Scanning－Electron－Microscopy
Specimen Preparation for Scanning Electron MicroscopyWe recommend consultation with one of the lab directors before preparing specimens. The methods p
Negative－Stain－Electron－Microscopy－of－Microtubules
Negative staining is a rapid, qualitative method for analyzing microtubule structure at the EM level. Because negative staining involves deposition of
Preparation－Of－Ciliated－Protozoa－For－Scanning－Electron－Microscopy
Preparation Of Ciliated Protozoa For Scanning Electron MicroscopyGeneral notes: The same procedures are used to fix and stain cells for SEM and for TE
免疫电镜(Immune－electron－microscopy)原理
（一） 原理 免疫电镜技术是免疫化学技术与电镜技术结合的产物，是在超微结构水平研究和观察抗原、抗体结合定位的一种方法学。它主要分为两大类：一类是免疫凝集电镜技术，即采用抗原抗体凝集反应后，再经负染色直接在电镜下观察；另一类则是免疫电镜定位技术。该项技术是利用带有特殊标记的抗体与相应抗原相结合，在电
免疫电镜(Immune－electron－microscopy)原理
（一） 原理免疫电镜技术是免疫化学技术与电镜技术结合的产物，是在超微结构水平研究和观察抗原、抗体结合定位的一种方法学。它主要分为两大类：一类是免疫凝集电镜技术，即采用抗原抗体凝集反应后，再经负染色直接在电镜下观察；另一类则是免疫电镜定位技术。该项技术是利用带有特殊标记的抗体与相应抗原相结合，在电子
SCI论文中怎样描述TEM结果？样品有怎样的形貌
鉴于有同学问我对于SCI论文写作这个部分的更新计划，我在这里稍微跟大家说一下。这个部分肯定不仅仅是列出一些常规实验结果如何描述这么简单。现在发的这些呢，只是一些基础准备。在将这些准备工作做完了之后，我们会按照论文的结构，对每个部分进行详细讨论，尽可能把我们这些年学到的有用的东西分享给大家，给大家提供
TEM－Specimen－Preparation：Preparative－Techniques－for－the－TEM
For routine transmission electron microscopy (TEM), it is generally accepted that specimens should be thin, dry and contain molecules which diffract e
Fixation－of－Cells－Cultured－in－Transwell－Dishes
Fixation of Cells Cultured in Transwell DishesTranswell culture dishes are commonly used to culture cells so that the top and bottom of the cells can
形貌分析
形貌分析的主要内容是分析材料的几何形貌，材料的颗粒度，及颗粒度的分布以及形貌微区的成份和物相结构等方面。形貌分析方法主要有：光学显微镜(Opticalmicroscopy,OM)、扫描电子显微镜(Scanningelectron microscopy, SEM)、透射电子显微镜(Transmissi
常用无机材料分析方法
Elemental Analysis 元素分析Atomic absorption spectroscopy 原子吸收光谱Auger electron spectroscopy (AES) 俄歇电子能谱Electron probe microanalysis (EPMA) 电子探针微分析Electro
Fixation－of－Embryos
MEMFA Fix 10xMEMFA Salts 1 part 10x MEMFA salts 1 M MOPS 1 part 37% formaldehyde 20mM EGTA 8 parts water 10mM MgSO4
SCI论文中怎样描述TEM结果？TEM结果描述样品尺寸分布情况
实例一：A particle count taken from many such images, obtained from different regions of the sample, confirmed the presence of essentially monodispersed A
扫描透射电子显微镜简介
扫描透射电子显微镜(scanning transmission electron microscopy，STEM)既有透射电子显微镜又有扫描电子显微镜的显微镜。STEM用电子束在样品的表面扫描，通过电子穿透样品成像。STEM技术要求较高，要非常高的真空度，并且电子学系统比TEM和SEM都要复杂。
扫描透射电子显微镜的功能介绍
扫描透射电子显微镜(scanning transmission electron microscopy，STEM)既有透射电子显微镜又有扫描电子显微镜的显微镜。STEM用电子束在样品的表面扫描，通过电子穿透样品成像。STEM技术要求较高，要非常高的真空度，并且电子学系统比TEM和SEM都要复杂。
扫描透射电子显微镜的技术特点
扫描透射电子显微镜(scanning transmission electron microscopy，STEM)既有透射电子显微镜又有扫描电子显微镜的显微镜。STEM用电子束在样品的表面扫描，通过电子穿透样品成像。STEM技术要求较高，要非常高的真空度，并且电子学系统比TEM和SEM都要复杂。
stem是什么仪器？
扫描透射电子显微镜(scanning transmission electron microscopy，STEM)既有透射电子显微镜又有扫描电子显微镜的显微镜。STEM用电子束在样品的表面扫描，通过电子穿透样品成像。STEM技术要求较高，要非常高的真空度，并且电子学系统比TEM和SEM都要复杂。
Methanol－Fixation－for－Immunofluorescence
Methanol fixation works by precipitating proteins, and as such it is a quick method (2-5)minutes is enough time for most antibodies/proteins). Diffuse
Histochemistry－－Introduction
A few cell types are thin enough to be viewed directly in a microscope (algae, protozoa, blood, tissue cultures), but most tissues (kidney, liver, bra
Light－Microscopy
The light microscope, so called because it employs visible light to detect small objects, is probably the most well-known and well-used research tool
ThiolReactive－Probe－Labeling－Protocol
实验概要 Invitrogen offers several fluorescent and biotinylated phalloidin and phallacidin derivatives for labeling F-actin. These phallotoxins, isola