USEOFTHELIGHTMICROSCOPE
USE OF THE LIGHT MICROSCOPEEach time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjust the iris diaphragm and focus the condenser to give its optimum performance, this could result in objects in the preparation being undetected.There are many microscope set up procedures for specific functionality but the most versatile for general use is ‘Critical Illu......阅读全文
CREATION－AND－USE－OF－YOUR－INFECTIOUS－VECTOR
实验概要CREATION AND USE OF YOUR INFECTIOUS VECTOR实验步骤Day 1 1. Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 mL of media. (This can be scal
Creation－and－Use－of－Infectious－Virus－Vector
Creation and use of your infectious vector:Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 ml of media. (This can be scaled up if desired).The f
Immunofluorescence－Labeling－of－Cells
实验概要Antibodies are an important tool for demonstrating both the presence and the subcellular localization of an antigen. Cell staining is a very ver
Differential－Interference－Contrast
Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)PrincipleDifferential interference microscopy requires several optical
Silane－and－PolyLLysine－Coating－of－Microscope－Slides
1) Wash slides in Decon solution for 30 minutes.2) Wash slides in running tap water for 30 minutes.3) Wash slides in distilled water for 2 x 5 minutes
How－to－perform－automated－counts－of－fluorescently－stained－cells.
NoteThis protocol describes semi-automated cell counts using fluorescently labeled cells, a hemocytometer and ImageJ software. The hemocytometer is n
Tissue－Culture－Methods1
I. TYPES OF CELLS GROWN IN CULTURE Tissue culture is often a generic term that refers to both organ culture and cell culture and the terms are often
使用量子级联激光器的可调谐长波红外陷波滤波器的...3
6.Performance Characterization ExperimentThe prototype filters were fabricated based on the device design shown in Fig. 3 and the specifications liste
Hippocampal－Neuron－Cultures
实验概要The protocol provides a method of hippocampal neuron cultures.主要试剂Begin by timing the pregnant mouse at E17-E19 days of gestation. Have ready the
HOW－TO－USE－THE－COULTER－COUNTER－TO－COUNT－CELLS
1) Turn on the counter by pulling out the on/off button. You need to do this at least 10 min before use to obtain sufficient vacuum. Usually put 0.
Guidelines－for－the－Use－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal
What is Anesthesia? Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are analgesia (pain relief), amn
Tissue－Culture－Methods2
IV. MAINTENANCECultures should be examined daily, observing the morphology, the color of the medium and the density of the cells. A tissue culture log
Hints－and－precautions－for－the－care,－feeding－and－breeding－of－Neurospora2
9. Spontaneous germination. Some genotypes result in spontaneous ascospore germination (for example, the unpigmented ascospores of per-1 Type I). A s
Microscopy－with－Oil－Immersion
Microscopy with Oil ImmersionPrincipleWhen light passes from a material of one refractive index to material of another, as from glass to air or from a
Basic－Theory－and－Use－of－GCMS（二）
For ionisation to take place at all, chemical reaction between the sample and the reagent gas must be exothermic. The grater the heat of the reaction
General－Laboratory－Procedures,－Equipment－Use,－and－Safety－Considerations
A. Storage . The following properties of reagents and conditions are important considerations in processing and storing DNA and RNA. Heavy metals pr
Use－of－the－Bradford－Protein－Assay－in－a－Microtiter－Plate－Format
Introduction The Bradford protein assay is a simple procedure for determination of protein concentrations in solutions that depends upon the change
Basic－Theory－and－Use－of－GCMS（一）
BASIC THEORY AND USE OF GC-MSbyDr. Eugenia SobolevaContent1. Introduction.2. GC-MS systems and components.3. Vacuum system3.1. Rotary pump3.2. Di
Live－imaging－with－Drosophila－tissue－culture－cells1
Introduction Live imaging provides an important complementation to the "snapshot" view obtained in fixed tissue by immunofluorescence. It allows fol
Spectrophotometry
A spectrophotometer or colorimeter makes use of the transmission of light through a solution to determine the concentration of a solute within the so
Appendix－G:－Spectrophotometry
Figure G.1 Electromagnetic Radiation SpectrumFigure G.2. Schematic light transmissionFigure G.3. Use of the Spec 20A spectrophotometer or colorimeter
Use－of－Nonaqueous－Fractionation－and－Metabolomics－to－Study－Chloroplast－...
Use of Non-aqueous Fractionation and Metabolomics to Study Chloroplast Function in Arabidopsis Chloroplasts are the chemical factories of plant cell
Use－of－the－B.D.－FACS－or－Calibur－Flow－Cytometers
Reservations:Schedule time on a cytometer (FACS or Calibur) by filling in the calendar at the flow lab. Don't grossly overbook since we have to pa
How－to－use－Basic－Local－Alignment－Search－Tool－(BLAST)
DescriptionThe BLAST algorithm was developed as a way to perform DNA and protein sequence similarity searches by an algorithm that is faster than FAST
原代神经细胞培养方法－Neuron－Cell－Culture
1. Preparation of coverslips1.1- Mass cultureOur standard mass cultures are plated on astrocytes. Those, in turn, are plated on glass coverslips pre-
XC－Assay－of－MoMLV－Virus－Stocks
MaterialsWildtype MoMLV virus aliquot. Stored at -80ºC.Medium: DMEM + 10% FBSNIH 3T3 TK- cellsXC cellsPolybrene 1000x stock = 4 mg/mL, sterile filte
可见光检测器－visible－light－detector
可见光检测器 visible light detector 又称分光光度检测器，是基于溶质分子吸收可见光的原理设计的检测器。能够直接采用可见光检测的溶质不是很多，而且多数灵敏度也不高，但采用具有高摩尔吸光系数的有机试剂（配位体和螯合剂）作为衍生化试剂进行柱前或柱后衍生操作的衍生化光度检测法是相当
梅特勒托利多LabX－Light－滴定软件
LabX Light 滴定软件产品详细说明 描述仪器控制功能：滴定仪控制在线曲线、测量值、一阶导数和结果直接控制滴定仪的辅助功能：搅拌、馈液、样品转换器的转动、电位测量每次分析后自动打印结果（用户可自定义内容）自动将结果导出到本地或网络硬盘（用户可自定义内容）
杂散光(Stray－light－)对吸光度的影响
一、杂散光的重要性杂散光是紫外可见分光光度计非常重要的关键技术指标。它是紫外可见分光光度计分析误差的主要来源, 它直接限制被分析测试样品浓度的上限。当一台紫外可见分光光度计的杂散光一定时, 被分析的试样浓度越大, 其分析误差就越大。ASTM 认为: “杂散光可能是光谱测量中主要误差的来源。尤
原子力显微镜（atomic－force－microscope,－AFM）工作原理
原子力显微镜是利用检测样品表面与细微的探针尖端之间的相互作用力（原子力）测出表面的形貌。探针尖端在小的轫性的悬臂上，当探针接触到样品表面时，产生的相互作用，以悬臂偏转形式检测。样品表面与探针之间的距离小于3－4nm，以及在它们之间检测到的作用力，小于10－8N。激光二极管的光线聚焦在悬臂的背面上。当