USEOFTHELIGHTMICROSCOPE
USE OF THE LIGHT MICROSCOPEEach time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjust the iris diaphragm and focus the condenser to give its optimum performance, this could result in objects in the preparation being undetected.There are many microscope set up procedures for specific functionality but the most versatile for general use is ‘Critical Illu......阅读全文
Immunostaining－of－Paraffin－Sections
Procedure: 1) Fix tissues for 3 hr on ice in 4 formaldehyde (2.5 ml of Polysciences #18814 made up to 10 ml in 80 mM NaPO4 [3.2 ml of 1 M NaPO4] pH 6.
Immunocytochemistry...
实验概要The method provides a guideline procedure for staining of cell cultures using immunofluoresence.实验步骤1. General procedure 1) Coat coverslips wit
Autoradiography
MaterialsH-Thymidine, specific activity of 2.0curie/millimoleOnion sets, jars and toothpicksAlcohol-acetic acid fixativeMaterials for feulgen reaction
Wound－healing－assay
The wound healing assay allows the researcher to study cell migration and cell interactions. In some cases also single cell migration can be analyzed.
Mapping－Protein－Distributions－on－Polytene－Chromosomes－by－Immunostaining2
12. Hold the salivary glands at the common duct with tweezers.Transfer the glands to a drop of fixing solution on a siliconizedcoverslip. 13. Incubate
Green－Fluorescent－Protein－as－an－Indicator－ofTransfection－in－Chicken－Embryos
Green fluorescent protein (GFP) is responsible for the bioluminescence of the Pacific Northwest jellyfish, Aequorea victoria. In A.victoria, the 27-kD
Isolation－of－Total－RNA－from－Animal－Cells－use－RNeasy－Mini－Kit
实验概要Extract the total RNA from animal cells by using RNeasy Mini Kit (QIAGEN No.74104) 主要试剂SDS based extraction solution实验步骤1. Harvest cells.1) Try
Use－of－the－B.D.－FACS－or－Calibur－Flow－Cytometers2
Gating:Gating will allow you to view cells of interest by any combination of criteria that you choose. Gating does not change the intensity value assi
Light最新影响因子13.625,影响力持续稳定提升
费城时间2018年6月26日，科睿唯安发布了最新的《期刊引证报告》(Journal Citation Reports 2017，简称JCR 2017)。中国科学院长春光学精密机械与物理研究所主办的期刊Light: Science & Applications (《光：科学与应用》，以下简称Light
EGel®－CloneWell－Agarose－Gels
实验概要Instructions are provided below for using the E-Gel®CloneWell pre-cast agarose gels with the E-Gel® iBase™ Power System. For detailed instructio
Guide－to－Cell－Proliferation－and－Apoptosis－Methods
Chapter 1: Cell Death - Apoptosis and Necrosis1.1Introduction21.1.1Terminology of cell death21.1.2Differences between necrosis and apoptosis31.1.3Apop
Subculturing－Adherent－Cells
实验概要The following protocol describes a general procedure for subculturing adherent mammalian cells in culture.主要试剂1. Complete growth medium, pre-warme
DAPI－Nucleic－Acid－Stain
实验概要The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove. Binding
Spectrophotometry——2
OPERATION OF B&L SPEC. 20The most commonly encountered spectrophotometer is one manufactured by Bausch and Lomb and known as the Spec 20. The 20 refer
Isolation－and－growth－of－pulmonary－artery－adventitial－fibroblasts
1. Adventitia from the main pulmonary artery was harvested neonatal calves. 2. Tissue was collected, carefully dissected free of blood vessels and f
Exposing－gels－and－plates－containing－radioactive－samples－to－Xray－film
Although most people use the PhosphorImager for western blots, kinase assays and methionine-labeled samples, X-ray film remains the best way to expose
安捷伦科技收购拉曼光谱供应商Cobalt－Light－Systems
分析测试百科网讯 2017年7月7日，安捷伦科技公司宣布以4000万英镑现金收购位于英国牛津的拉曼光谱仪器供应商Cobalt Light Systems，该公司的产品针对制药行业应用市场和公共安全。 Cobalt的台式及手持式/便携式拉曼光谱仪器产品组合基于专有技术，能够通过屏障识别化学品和材
Measurement－of－Carbon－Fixation－Rates－in－Leaf－Samples
Generation of a Light Curve To address the hypothesis concerning photosynthetic efficiency it is necessary to expose sun and shade leaves to a ran
Rat－Follistatin,FS－ELISA－Kit（大鼠卵泡抑素(FS)ELISA试剂盒）使..
Rat Follistatin,FS ELISA Kit使用原理大鼠卵泡抑素(FS)ELISA试剂盒货号:FK-QZ1936规格：96T/48TThe kit was a solid phase sandwichenzyme-linked immunosorbent assay (ELISA
Alexa－Fluor®－488－Annexin－V/Dead－Cell－Apoptosis－Kit
实验概要Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development. Inappropriately regulated apoptosis is imp
Fluorescent－Staining－of－Cells
1. Fluorescent phalloidin in methanol. Phallacidin does not work as well. Dilute 10 ul 330 nM stock into 500 ul PBS for each large coverslip.2. PB
Crosses
Weed out the plant pot, leave only about 3 to 8 tall and vigorous plants (in a regular 5 cm pot).On an individual inflorescence, cut off all the ope
新技术：InCell－Western－Assay2
III. Experimental ConsiderationsProper selection of microplates can significantly affect the results of your analysis, as each plate has its own chara
Histochemical－staining－of－sea－urchin－embryos－for－(AP)－enzyme－activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity1. Obtain embryo samples, tube of AP substrate buffer and tu
Protein－Immunolocalization－in－Maize－Tissues
The analysis of gene expression at transcript and at protein level is of outstanding importance in plant developmental biology. Proteins can be loc
Mouse－Renin－ELISA－Kit英文使用说明
Mouse Renin ELISA Kit使用原理 小鼠肾素(Renin)ELISA试剂盒货号:FK-QZ3075规格：96T/48TMouse Renin ELISA Kit使用原理The kit was a solid phase sandwichenzyme-linked immunoso
Mouse－Pulmonary－surfatcantassociated－protein－A,SPA－ELISA－Kit使用说明
Mouse Pulmonary surfatcant-associated protein A,SP-A ELISA Kit使用原理小鼠肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒货号:FK-QZ3080规格：96T/48TMouse Pulmonary surfatcant-associa
小鼠白介素17(IL17)ELISA试剂盒英文使用说明
Mouse Interleukin 17,IL-17 ELISA Kit使用原理小鼠白介素17(IL-17)ELISA试剂盒货号:FK-QZ2901规格：96T/48TMouse Interleukin 17,IL-17 ELISA Kit使用原理The kit was a solid phas
小鼠神经生长导向因子Slit2ELISA试剂盒英文使用说明
Mouse Neuronal Chemorepellent Slit2 ELISA Kit使用原理 小鼠神经生长导向因子Slit2ELISAKit货号:FK-QZ2950规格：96T/48TMouse Neuronal Chemorepellent Slit2 ELISA Kit使用原理The
小鼠红细胞生成素(EPO)ELISA试剂盒英文使用说明
规格： 96T/48TMouse Erythropoietin,EPO ELISA Kit使用原理 小鼠红细胞生成素(EPO)ELISA试剂盒货号:FK-QZ2925规格：96T/48TMouse Erythropoietin,EPO ELISA Kit使用原理The kit was a so