CGHProtocols（三）
Hybridizationreagents: labeled tumor and normal-DNA (see protocol Nick translation) salmon sperm DNA, 10 mg/ml (e.g. Promega) human Cot1 DNA, 1 mg/ml (GibcoBRL, Life Technologies) 3M sodium acetate 100% ethanol, 90% ethanol, 70% ethanol Formamide (FA), (e.g. from Merck) Master mix (20% dextransulfate/4xSSC) SSC (20x) 70% FA/2xSSC for chromosome denaturation (120 µl per sli......阅读全文
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