NoninvasiveMicroelectrodeIonFluxEstimationTechnique(MIFE)forthe...
Noninvasive Microelectrode Ion Flux Estimation Technique (MIFE) for the Study of the Regulation of Root Membrane Transport by Cyclic NucleotidesChanges in ion permeability and subsequently intracellular ion concentrations play a crucial role in intracellular and intercellular communication and, as such, confer a broad array of developmental and adaptive responses in plants. These changes are mediated by the......阅读全文
Noninvasive－Microelectrode－Ion－Flux－Estimation－Technique－(MIFE)－for－the...
Noninvasive Microelectrode Ion Flux Estimation Technique (MIFE) for the Study of the Regulation of Root Membrane Transport by Cyclic NucleotidesCh
同时测定小麦原生质体的电流与离子流，发现电流与K+...
同时测定小麦原生质体的电流与离子流，发现电流与K+密切相关，与Ca2+无关关键词：微电极离子流测定 ( Microelectrode Ion-Flux Estimation); 膜片钳（Patch clamp）参考文献：Matthew G. et al. The Plant Journal. 200
大肠杆菌跨膜H+、Ca2+、K+、NH4+流的变化
关键词：大肠杆菌（E.coli）; 氢; 离子流（ion flux）; 微电极技术（Microelectrode technique）参考文献：Shabala L, et al. J Microbiol Methods,2001, 46:119-129大肠杆菌跨膜H+、Ca2+、K+、NH4+流的变
蓝光诱导黄化野生型和向光素突变体拟南芥幼苗的H+和...
蓝光诱导黄化野生型和向光素突变体拟南芥幼苗的H+和Ca2+流 AbstractIon flux kinetics associated with blue light (BL) treatment of two wild types (WTs) and the phot1, phot2 and ph
Quantifying－Kinetics－of－Net－Ion－Fluxes－from－Plant－Tissues－by－Noninvasi...
Non-invasive microelectrode ion flux measuring (the MIFE system) allows concurrent quantification of net fluxes of several ions with high spatial
选择性微电极在植物生理学研究中的应用（五）
7 展望选择性微电极技术能用于直接并灵敏地观察植物体对矿质元素的需求，研究者可利用选择性微电极技术进行对植物某种离子或高或低的吸收的品种的筛选，还可制定出与植物需求相适应的环境的营养水平；能及时准确地探测到的光、温、水涝、盐分引起的植物体离子或分子信息的微小变化，能成为预测植物受到逆境胁迫最直观、
选择性微电极在植物生理学研究中的应用（一）
朱俊英1，高荣孚1，许越2,3*1北京林业大学生物科学与技术学院，北京100083；2旭月（北京）科技有限公司，北京100080；3Vibrating Probe Facility,Biology Department,University of Massachusetts at Amherst,M
非损伤检测胎儿肺上皮细胞微环境中Cl流的变化情况
肺上皮细胞中体液运输由上皮细胞膜两侧渗透压产生的Na+和Cl-流调节支配，而离子转运过程中所需的能量是由活性Na+/K+ATP酶所提供，Na+和Cl-的跨膜分布是由配体调节的离子通道和交换器所控制，所以如果Cl-的转运发生缺陷将导致与体液运输失调相关的疾病。囊性纤维病的发生就是由于Cl-转运的缺失而
植物膜转运的模型预测、实验验证及生理学影响
图注：上图：玉米根部O2流的振荡变化作为O2利用情况的指标。A:不同氧气浓度下O2流的振荡规律；B:O2流振荡对外界氧气的依赖性。关键词：适应(Adaptation)；离子流(Ion flux)；膜(Membrane)参考文献：Shabala S et al. . J. Exp. Bot. .200
非损伤性扫描离子选择电极技术及其在高等植物...（三）
图3 H+和O2 流动速率的同时测量.(a)显微照片显示金属氧电极与玻璃H+电极同时测量百合花粉管生长过程中H+离子和O2分子进出的变化；(b)在花粉管线粒体密集区域, 或称固有碱化带区域，同时存在的H+外流和O2内流现象. 2.2 SIET与荧光显微技术结合证明磷脂酰肌醇转运蛋白与根毛发生有
非损伤性扫描离子选择电极技术及其在后基因...（十一）
4. 总结及展望SIET在借鉴众多科学家工作经验的基础上，经过多年的改进和完善，为科研人员提供了一个较为友好的软硬件环境，在数据的生成，采集以及校准等方面，极大地方便了研究人员。特别是应用SIET强大的3维立体测量方式，研究人员可以获得其他电生理技术无法测到的被测样品某些点的特异活性 [10]。对于
膜片钳技术（patch－clamp)
Instruments For ElectrophysiologyProducts include microelectrode, voltage and current clamp amplifiers, perfusion chambers, perfusion heating systems,
Estimation－of－Fat－in－Milk－Samples
实验概要Milk fat measurement is a common task in the dairy industry, because milk fat content is one factor that determines milk price and is necessar
Estimation－of－Fat－in－Milk－Samples
IntroductionMilk fat measurement is a common task in the dairy industry, because milk fat content is one factor that determines milk price and is nece
异搏定和钆对青蛙骨骼肌纤维中咖啡因诱导的痉挛和钙...
异搏定和钆对青蛙骨骼肌纤维中咖啡因诱导的痉挛和钙离子流的影响许多研究证明咖啡因可以诱导骨骼肌的收缩，但Axelsson等科学家在青蛙的骨骼肌中注入咖啡因后却未发现收缩现象。面对这种现象，澳大利亚Tasmania大学的Lana Shabala等科学家推测咖啡因引起的青蛙慢骨骼肌纤维收缩的活性位点在胞外
Calcium－Flux－Measurement－Using－Indo1
实验概要Measure the calcium in cytosol with indo-1.主要试剂DMSO, Imonomycin, RPMI1640, PBS实验步骤1. Dissolve the contents of 1 vial (50 μg indo-1) in 50 μl dry
Sterile－Technique
Good sterile technique is the first and most important step in insuring consistent results when employing recombinant DNA and protein expression techn
Immunofluroescence－Technique
Fix cells in 2% formaldehyde in PBS/pH 7.4 for 15 min. at 20oC. 2% formaldehyde is made up fresh prior to use by dissolving the appropriate amount of
Assay－of－superoxide－dismutase－activity3
Botanical Bulletin of Academia Sinica, Vol. 37, 1996The method using NBT as a superoxide radical competitor and a color indicator was also explored to
抗凋亡基因（CED9）提高植株对盐胁迫和氧化应激的耐...
抗凋亡基因（CED-9）提高植株对盐胁迫和氧化应激的耐受性凋亡(Apoptosis)是细胞程序性死亡的一种，在调节植物对环境的适应性中起到重要作用。近期有研究表明动物的抗凋亡基因(CED-9)在植物中表达，能够显著提高植物对各种生物和非生物胁迫的耐受性，但隐藏在该现象下的最基本的细胞机制尚未被考察。
Noninvasive－Human－Nuclear－Transfer－with－Embryonic－Stem－Cells
Noninvasive Human Nuclear Transfer with Embryonic Stem CellsSohyun L. McElroy1 and Renee A. Reijo PeraCenter for Human Embryonic Stem Cell Research an
Principles－of－Aseptic－Technique
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
海洋原生生物破囊壶菌的渗透调节和对Na+的需求
破囊壶菌是低等的真菌，生长在大量的Na+环境中。 Na+参与细胞渗透调节和细胞代谢。渗透调节通过从环境中吸收无机离子，或者改变细胞质中可溶性物质的浓度来完成。通过质膜的渗透调节在转运过程中非常重要。但是在海洋原生生物中如何通过质膜进行渗透调节还不清楚。澳大利亚的科学家Shabala等人用非损伤微测技
a－pipeline－for－the－identification－of－intact－Nglycopeptides（六）
Figure 4. Comparison of different decoy strategies. The estimated FDR of each decoy method is compared with the real FDR. “Decoy: + 11” means in
Principles－of－Aseptic－Technique——3
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
Principles－of－Aseptic－Technique——3
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
ALKALINE－PHOSPHATASE－(APAAP)－TECHNIQUE
Preparation: Cytological PreparationsFixation: Air dry films or cytospin preparations overnight at room temperature. For frozen and paraffin sections
Principles－of－Aseptic－Technique——2
PersonnelAseptic technique requires careful attention to a series of steps which begins with patient and instrument preparation and ends at final woun
Principles－of－Aseptic－Technique——4
P REPARATION OF THE SURGEONIn a laboratory setting, the extent of surgeon preparation will depend on the facilities and the need for strict attention
恩他卡朋促进大鼠cAMP依赖的Cl分泌引起胃肠不适
恩他卡朋（Entacapone）是一种广泛用于帕金森疾病（PD）治疗的药物，是儿茶酚-O-甲基转移酶（COMT）的抑制剂。然而，恩他卡朋有胃肠副作用，临床上约10%的服用者会出现腹泻。为了研究产生腹泻的机理，科学家采用的新方法非损伤微测技术结合传统方法研究了恩他卡朋造成腹泻的原因。2011年，首都医