Twohybridanalysisofgeneticregulatorynetworks2
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey protein and hundreds of baits (Protocol 3, see Figure 1 below). Large panels of bait strains can be collected and stored frozen indefinitely (Protocol 2) and then screened against any number of preys. One such set of bait strains contains over 700 different LexA fusion......阅读全文
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