TheEffectsofUltravioletLightontheFertilization
Jill K. Flemming, Franklin & Marshall College, Class of 2001IntroductionThe objective of this project is to observe the effects of UV radiation on both fertilization and development in the sea urchin embryo.Ultraviolet (UV) light is becoming an increasing concern in today's society. Humans, as well as other organisms, are being exposed to more and more UV rays due to the expanding hole in and thinning of Eart......阅读全文
The－Effects－of－Ultraviolet－Light－on－the－Fertilization
Jill K. Flemming, Franklin & Marshall College, Class of 2001IntroductionThe objective of this project is to observe the effects of UV radiation on bot
In－Vitro－Fertilization
When we first started using X. tropicalis, in vitro fertilization had an extremely poor efficiency. However, with the careful selection of a mature ma
Ultraviolet－irradiation－impairs－epiboly－via－microtubules－in－Zebrafish
IntroductionZebrafish have transparentembryos that develop outside the mother. They develop rapidly, so that at 24 hours after fertilization, the embr
Sea－Urchin－Fertilization
Gametes are collected as described. To remove some of their jelly coat,wash eggs several times in ASW by allowing them to settle and gently pouring of
Light－Microscopy
The light microscope, so called because it employs visible light to detect small objects, is probably the most well-known and well-used research tool
USE－OF－THE－LIGHT－MICROSCOPE
USE OF THE LIGHT MICROSCOPEEach time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjus
Measurement－with－the－Light－Microscope
Measurement with the Light MicroscopeYour microscope may be equipped with a scale (called a reticule) that is built into one eyepiece. The reticule ca
The－Effects－of－Ethanol－on－Zebrafish－Development
Introduction:The early stages of zebrafish, Danio rerio development are characterized by meroblastic dicoidal cleavage. Initially cell division only o
Developmental－Effects－of－Transplantation－of－Cell
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mos
Effects－of－calcineurin－in－Keratinocyte－Differentiation
The differentiation of keratinocytes constantly replenishes the upper layers of human skin we lose each day. One factor that contributes to terminal k
The－effect－of－temperature－on－the－embryonic－development－of－sea－urchins
Objective: This experiment aims to discover if temperature has an effect on the rates of sea urchin embryonic development. By altering the external en
The－Developmental－Effects－of－Nicotine－on－Chick－Embryos
More than 22 million American women smoke and approximately 25% of these women continue to smoke during pregnancy (Lambers and Clark 1996). Cigarette
Determining－the－effects－of－nicotine－on－bone－－in－chicks
ObjectiveThe purpose of this experiment is to determine how the development of bone and cartilage in a chick are affected by treating the embryo with
Effect－of－UV－Treatment－on－Early－Development－of－Sea－Urchin－Embryos
Objective:To test the effects of UV radiation at 254nm on Sea Urchin embryo development after radiation at the 2-cell stage.Background:Sea Urchins exh
紫外吸收检测器－ultraviolet－absorption－detector
紫外吸收检测器 ultraviolet absorption detector 简称紫外检测器（UV），是基于溶质分子吸收紫外光的原理设计的检测器。因为大部分常见有机物质和部分无机物质都具有紫外吸收性质，所以该检测器是液相色谱中应用最广泛的检测器，几乎所有液相色谱仪都配置了这种检测器。它不仅有较
The－effects－ofdifferent－concentrations－oflithium－chloride－on－thedevelopment
AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular Brachydanio rerio embryos results in
DEVELOPMENTAL－EFFECTS－OF－LITHIUM－CHLORIDE－ON－XENOPUS－EMBRYOS
PURPOSE: The objective of this experiment is to study the effect of lithium chloride treatment on early blastula xenopus embryos.INTRODUCTION: When th
The－Effects－of－NiCl2on－Spicule－Formation
The Effects of NiCl2on Spicule FormationJessica Ann Billet, Franklin and Marshall, Class of 2000Background and ObjectiveSea urchins exhibit radial hol
Basic－mechanism－of－action－of－PPARa,－PPARb(d)－and－PPARg－and－effects
Similar to other nuclear hormone receptores, PPAR acts as a ligand activated transcription factor. Upon binding fatty acids or hypolipidemic drugs, PP
Spectrophotometry
A spectrophotometer or colorimeter makes use of the transmission of light through a solution to determine the concentration of a solute within the sol
Use－of－SemiThin－Cryosections－for－Light－Microscopy.
Use of Semi-Thin Cryosections for Light Microscopy.Semi-thin sections can be obtained from frozen blocks of cryoprotected biological material by secti
The－Effects－of－CalciumFree－Seawater－onthe－Development－ofSea－Urchin－Embryos
ObjectiveThe purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were pl
Microscopes－in－Cell－Biology
Microscopes in Cell BiologyIntroductionMicroscopy has a major role in the study of cells. From the very beginning, researchers have tried to develop w
梅特勒托利多LabX－Light－滴定软件
LabX Light 滴定软件产品详细说明 描述仪器控制功能：滴定仪控制在线曲线、测量值、一阶导数和结果直接控制滴定仪的辅助功能：搅拌、馈液、样品转换器的转动、电位测量每次分析后自动打印结果（用户可自定义内容）自动将结果导出到本地或网络硬盘（用户可自定义内容）
可见光检测器－visible－light－detector
可见光检测器 visible light detector 又称分光光度检测器，是基于溶质分子吸收可见光的原理设计的检测器。能够直接采用可见光检测的溶质不是很多，而且多数灵敏度也不高，但采用具有高摩尔吸光系数的有机试剂（配位体和螯合剂）作为衍生化试剂进行柱前或柱后衍生操作的衍生化光度检测法是相当
杂散光(Stray－light－)对吸光度的影响
一、杂散光的重要性杂散光是紫外可见分光光度计非常重要的关键技术指标。它是紫外可见分光光度计分析误差的主要来源, 它直接限制被分析测试样品浓度的上限。当一台紫外可见分光光度计的杂散光一定时, 被分析的试样浓度越大, 其分析误差就越大。ASTM 认为: “杂散光可能是光谱测量中主要误差的来源。尤
Appendix－G:－Spectrophotometry
Figure G.1 Electromagnetic Radiation SpectrumFigure G.2. Schematic light transmissionFigure G.3. Use of the Spec 20A spectrophotometer or colorimeter
Gamry电化学工作站：拉曼光谱电化学基础
Gamry电化学工作站：拉曼光谱电化学基础Purpose of This NoteThis application note discusses Raman spectroscopy and its combination with electrochemical techniques.The th
Inhibition－of－the－fast－and－slow－blocks－to－polyspermy
Objective: to test the effects of inhibiting the fast and slow blocks to polyspermy on sea urchin development.BackgroundSea urchin eggs release compon