决定阴阳性的CUTOFF值究竟是怎么来的？
定性免疫检测中，决定结果阴阳性的依据是CUT OFF值。确定合适的CUT OFF值（阳性判断值）对于准确判定结果有重要意义。CUTOFF值的确定要使依其得到的检测结果假阴性和假阳性的发生率最低。那么，CUT OFF值是怎么来的？什么是“灰区”？ 在介绍怎样设定CUT OFF值前，先介绍灰区的概念。定性检测将结果区分为阴性、阳性。如果阴性样本的检测结果分布与阳性结果完全分开没有重叠，那进行区分时就非常方便也没有争议。在实际检测过程中，检测大量阴性样本与阳性样本后，会有少量样本测值有重叠。这一部分样本的检测结果归为可疑，即“灰区”。 CUT OOFF值的设定CUT OFF值的设定有多种不同方法。常见的方法包括：一、使用阴性血清测定结果均值的2或3倍作为CUT OFF值该方法是取一定数量（通常不多）的阴性血清样本，使用免疫检测试剂盒进行测定，取阴性样本的测值的平均值。若上述阴性样本的平均值为X，则该次测定的CUT ......阅读全文
Dissociation－of－spleen－and－hemopoietic－tissue
You need to buy glass slides with frosted, sandblasted ends (Fisher Scientific, Catalog N. 12-552). Frosting by painting (e.g.Superfrost) should not
肌红蛋白测定的临床意义具体是什么
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人（Human）抗弓形体IgM抗体（antitox－IgM）ELISA检测试剂...
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NuPAGE GelsA gel electrophoresis system used for SDS-PAGE protein analysis. The gels are made up of Bis-Tris-HCl (pH 6.4) polyacrylamide and are inten
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1. Adventitia from the main pulmonary artery was harvested neonatal calves. 2. Tissue was collected, carefully dissected free of blood vessels and f
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The method we use is based on work of Dr. Hazel Cheng, at the University of Toronto and works for both colon and the small intestine.First we excise t
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Electrophoresis of PCR products with Life Technologies Sunrise gel apparatusGel: In a 500 ml Pyrex® glass bottle, add:Agarose:3 gH2O270 mls10X TA30 ml
乙肝检测单位竟然有9种
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For routine transmission electron microscopy (TEM), it is generally accepted that specimens should be thin, dry and contain molecules which diffract e
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Phosphoamino acid analysis:Mark Kamps's method1. Label your protein with 32Pi. Then subject the protein to SDS polyacrylamide gel electrophoresis
抗体纯化
Antibody PurificatioinPurification of IgG Using Protein A- or Protein G-Agarose (KPL) Purifying Antibodies (Perkin-Elmer)Precipitation MethodsProtein
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1. Human tissue was derived from atrial or ventricular biopsy specimens belonging to patients undergoing heart surgery.2. Isolated myocardial tissue w
Pathology－and－Autopsy－of－a－mouse
if mouse is found deadif mouse is a newborn or < 1 wk of ageIf mouse is alive or moribund and above one week of ageRoutine dissectionFormalin fixation
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Exercise 12.10 - Establishment of a Primary CultureLEVEL IIIMaterialsChick embryo (approximately 8 days old)70% (v/v) ethanol for swabbingSterile scis
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肌红蛋白测定的参考值、注意事项和临床意义
临床意义血清肌红蛋白增高：常见于急性心肌梗死早期、骨骼肌和神经肌肉病、肾衰、肌肉注射等。还可见于缺血性心脏病、心绞痛、心肌损伤、心源性休克、出血性休克、肌营养不良症。另外，烧伤、酒精中毒、糖尿病酸中毒、甲状腺功能减退症时，血清肌红蛋白水平也增高。正常值参考范围血清肌红蛋白正常参考值：12～75ng/
immunofluorescence－of－rabbit－antimurine－leptin－by－Peprotech
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I. Purpose:A. Samples of solid tumors or lymph nodes may be sent from patients with cancer. These samples should be processed directly and also set up
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1. Run the gel as normal. Often for genomic southerns it is desirable to run long gels (18cm) over 4-6hrs.2. Photograph the gel with a ruler adjacent
Southern杂交技术
SOUTHERN BLOT1. Run the gel as normal. Often for genomic southerns it is desirable to run long gels (18cm) over 4-6hrs.2. Photograph the gel with a r
UV－CrossLinking－an...－（一）
实验概要Interest in RNA-protein interactions is booming as we begin to appreciate the role of RNA, not just in well-established processes such as trans
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手动式抛光机是由机座、主轴机头、抛光主轴、动力传动机构、张紧机构、马达等组成。抛光机可使用千页砂轮、麻轮、尼龙轮、布轮、羊毛轮等磨轮对各种材料工件、零件、组件实施表面加工，使用该类型机器进行粗抛(Cutting)、中抛(Cut & Colouring)、精抛(Colouring)、超精光抛(Su
immunofluorescence－of－rabbit－antimurine－RELMβ－by－Peprotech
实验概要The following protocol provides a method of immunofluorescence of rabbit anti-murine RELMβ by Peprotech.实验步骤The following protocol used B6 mice
GFP骨的冰冻切片实验方法
Protocol for Frozen section of GFP Bone:Fix the bone in 4% Paraformaldehyde at 4�C under constant agitation for 3 days.Decalcification in 14% EDTA sol
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手动式抛光机是由机座、主轴机头、抛光主轴、动力传动机构、张紧机构、马达等组成。抛光机可使用千页砂轮、麻轮、尼龙轮、布轮、羊毛轮等磨轮对各种材料工件、零件、组件实施表面加工，使用该类型机器进行粗抛(Cutting)、中抛(Cut & Colouring)、精抛(Colouring)、超精光抛(Su
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Following PCR, you often want to get rid of the PCR primers and Taq polymerase before the next step. This is necessary for sequencing PCR products or
DNA甲基化分析
The influence of methylation on the promoter activity and gene expression and the involvement of DNA methylation in carcinogenesis caused an extensive
ImmunohistochemistyEnzymatic－Protocol
OverviewR&D Systems provides monoclonal, polyclonal and biotinylated antibodies for immunohistochemical use. The following protocol has been developed
In－vitro－culture－of－embryonic－lungs
In vitro culture of embryonic lungsfrom Hogan LabIsolation of Lung Bud EndodermWhat you need:E11-12 mouse embryosDMEM with 5% fetal bovine serumpetri