NuclearRunOnTranscriptionAssays
Nuclear “run-on” (or “run-off”) transcription assays have been used to obtain quantitative information about the relative rates of transcription of different genes in nuclei isolated from a particular tissue or organ. This information can then be used in analyzing the factors that control the expression of specific genes. These assays are complimentary to measurements of the levels of mRNA species present i......阅读全文
In－Vitro－prostate－colony－and－sphereforming－assays
1. Prostates were dissected, minced into small pieces with a steel blade, and digested with 0.8 mg/ml collagenase in 10 ml of primary cell medium/
GenomeWide－Identification－of－Transcription－FactorBinding－Sites－in...
Genome-Wide Identification of Transcription Factor-Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip)
The－Determination－of－Proteinprotein－Interactions－by－the－Matingbased－...
Dynamic and reversible protein–protein interactions have a pivotal function in all living cells. For instance, protein–protein interactions are i
转录分析的几种方法
信号通路，只有一个目的：将细胞的外部信号转换成细胞的内部变化。不管是胰岛素，还是异亮氨酸，抗原还是肾上腺素，它们的终极目的总是如出一则：对转录活性的改变。 这些对于转录活性的影响来自于转录因子与基因的调控区域：如启动子、增强子、沉默子等结合达到的。经典的凝胶迁移滞后试验（the electrop
Gel－Shift－Assay－Systems
ProtocolsDownloadprotocol183kbpdf?Abstract for Gel Shift Assay SystemsThe gel shift, or electrophoretic mobility shift, assay provides a simple and ra
人工转录因子的部件——人类锌指结构1
Human zinc fingers as building blocks in the construction of artificial transcription factorsKwang-Hee Bae1, 4, Young Do Kwon1, 2, 4, Hyun-Chul Shin1,
MicroRNA－Expression－Profiling－by－Bead－Array－4
Bead-array-based microRNA detection technology, including the bio-statistic analysis, is currently not well established or widely used and we have app
DNA－Immunoprecipitation－for－the－Determination－of－DNABinding－Specificity
Andrea J. Gossett and Jason D. Lieb1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA1Corresponding autho
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Guide－to－Cell－Proliferation－and－Apoptosis－Methods
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体外荧光法检测核内体早期动力学
A fluorescence-based in vitro assay for investigating early endosome dynamicsSina V Barysch1,2, Reinhard Jahn1 & Silvio O Rizzoli2ABSTRACTEarly endoso
Detection－of－MicroRNA－Heterogeneity－in－Single－Cells－Using－an－Automated
Introduction MicroRNA (miRNAs) are short (18–24 nucleotides), non-coding RNAs that regulate gene expression by both disrupting messenger RNA (mRNA
siRNAs结合生物芯片的实验设计1
Ambion and Applied Biosystems have joined forces to provide a complete convenient, solution for performing gene silencing experiments and validating
Phosphatidylinositol－4Kinase－and－Phosphatidylinositol－4Phosphate－5K...
Phosphatidylinositol 4-Kinase and Phosphatidylinositol 4-Phosphate 5-Kinase Assays Inositol lipid kinases are perhaps the easiest and most straightf
利用高通量微流体技术研究单细胞生物系统运作
简介在动态的环境里面，细胞们通过各遗传途径的相互作用交流运转着。哺乳动物免疫反应就是各类不同的细胞协同合作的一个惊人例子。细胞与细胞之间的交流主要是通过信号分子形成时间与空间浓度梯度来介导的，这就要求细胞对一个大范围内的信号强度产生响应。这篇文章采用高通量的微流体细胞培养(high-throughp
Chloroplast－Phenomics:－Systematic－Phenotypic－Screening－of－Chloroplast－...
Chloroplast Phenomics: Systematic Phenotypic Screening of Chloroplast Protein Mutants in Arabidopsis As part of a project to analyze chloroplast fun
Preparation－of－nucleic－acid－probes
Preparation of nucleic acid probesIn standard nucleic acid hybridization assays the probe is labeled in some way. Nucleic acid probes may be made as s
微生物所在小分子检测方法开发中取得进展
小分子的灵敏检测是临床诊断、环境监测和食品安全等领域的重要需求，因此开发高效的化学小分子检测方法具有重要意义。其中，特异的识别元件是实现检测的核心和基础，传统的检测方法是通过抗体、酶等作为识别元件和待检测物进行识别，然后再通过对识别信号的放大来实现检测。整个开发过程往往面临着周期长、难度大、信号
siRNAs结合生物芯片的实验设计2
Figure 2. Silencer ™ siRNA Validation Data Generated Using Applied Biosystems TaqMan® Gene Expression Assays. The indicated Silencer Validated siRNAs
Twohybrid－analysis－of－genetic－regulatory－networks
1. Introduction and BackgroundThere is a great need for general methods to characterize the proteins that contemporary biology makes available. The li
利用人工组合转录因子对人类基因组扫描
Scanning the human genome with combinatorial transcription factor librariesPublished online: 18 February 2003, doi:10.1038/nbt794March 2003 Volume 21
利用人工组合转录因子对人类基因组扫描2
Figure 5: Regulation of CDH5 by TFZFs in several human cancer cell lines.Blue, cells infected with a pMX construct containing the DNA binding domain o
Competitive－RTPCR－Strategy－for－Quantitative－Evaluation－4
We have also been able to detect expression of this receptor in all studied tissues, which is consistent with the pleiotropic nature of growth hormone
人工转录因子的部件——人类锌指结构2
Table 2: Binding sites and identity of ZFPs used in VEGF activationWe then generated artificial transcription factors by fusing the three-finger domai
Preparation－of－Stroma,－Thylakoid－Membrane,－and－Lumen－Fractions－from－...
Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic AnalysisFor many studies regarding
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Tyrosine Kinase Assay Using Synthetic Peptides (T. Miller)Small synthetic peptide substrates are especially well suited for applications such as assay
Multiple－studies－with－a－single－experiment:－The－Power－of－...（六）
•SCX and high pH reversed phase fractionation are both orthogonal to low pH C18 LC separation•Strong cation exchange (SCX) requires sample desalting
Dynamic－Flow－Assay－in－a－Parallel－Plate－Flow－Chamber
Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850Flow assays allow visualization of c
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The－Impact－of－Harmo...－（二）
In addition, proficiency panel projects have demonstrated that even after an experiment was done and spots were counted, considerable variation can