BasicTheoryandUseofGCMS(一)

BASIC THEORY AND USE OF GC-MSbyDr. Eugenia SobolevaContent1. Introduction.2. GC-MS systems and components.3. Vacuum system3.1. Rotary pump3.2. Diffusion pump3.3. Turbomolecular pump4. Interfacing GC and MS4.1. Packed column interfacing4.......阅读全文

Basic-Theory-and-Use-of-GCMS(一)

BASIC THEORY AND USE OF GC-MSbyDr. Eugenia SobolevaContent1.  Introduction.2.  GC-MS systems and components.3.  Vacuum system3.1.  Rotary pump3.2.  Di

Basic-Theory-and-Use-of-GCMS(二)

For ionisation to take place at all, chemical reaction between the sample and the reagent gas must be exothermic. The grater the heat of the reaction,

How-to-use-Basic-Local-Alignment-Search-Tool-(BLAST)

DescriptionThe BLAST algorithm was developed as a way to perform DNA and protein sequence similarity searches by an algorithm that is faster than FAST

Centrifugation-Theory

Centrifugation is a process used to separate or concentrate materials suspended in a liquid medium. The theoretical basis of this technique is the eff

Basic-handson-course-in-thermal-analysis-software-(English)

METTLER TOLEDO is pleased to announce their series of one-day training courses, which are designed to familiarize users with thermal analysis theo

Basic-PCR

实验概要The  following basic protocol serves as a general guideline and a starting  point for any PCR amplification. Optimal reaction conditions (incubati

Use-of-Micropipets

A. Gilson-style Pipetmen Micropipets (and similar models)We primarily use the Gilson micropipets in the core course labs. We have four sizes identifie

Use-of-a-Hemacytometer

A hemacytometer (also spelled hemocytometer) is an etched glass chamber with raised sides that will hold a quartz coverslip exactly 0.1 mm above the c

Basic-Mechanisms-of-SUMOylation

Like ubiquitin, SUMO (small ubiquitin-related modifier) proteins are small protein tags that are conjugated to proteins to modify their function. The

USE-OF-THE-LIGHT-MICROSCOPE

USE OF THE LIGHT MICROSCOPEEach time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjus

USE-诱变实验

实验材料 带 mutS 表型(例如BMH71-18) 的转化用大肠杆菌感受态带 mut+表型的转化用大肠杆菌感受态试剂、试剂盒 退火缓冲液贮存液合成缓冲液噬菌体 T4DNA 连接酶噬菌体 T4DNA 聚合酶或测序酶单一位点的限制性内切核酸酶琼脂糖凝胶诱变引物选择引物质粒 DNA仪器、耗材 70°C

USE-诱变实验

            实验材料 带 mutS 表型(例如 BMH71-18) 的转化用大肠杆菌感受态 带 mut+表型的转化用大肠杆菌感受态 试剂、试剂盒

USE-诱变实验

本方法中,两条寡核苷酸引物杂交到变性重组质粒 DNA 双链的同一条链上。一条引物(诱变引物)携带一个拟引进靶 DNA 序列的突变,第二条引物携带一个能破坏质粒单一限制酶位点的突变。本实验来源于分子克隆实验指南(第三版)下册,作者:〔美〕J. 萨姆布鲁克 D.W. 拉塞尔。实验材料带 mutS 表型(

Basic-ELISA-Protocol

实验概要        There are many different types of ELISAs, which can detect the presence of protein in serum or supernatent. One of the most common typ

Basic-Protein-Chemistry-Techniques

实验概要Basic Protein Chemistry Techniques实验步骤Coomassie Blue Stain:  (for gels) 1) Combine 225 ml Methanol with 225 ml ddH2O. 2) Add 0.5 grams of Coomassi

Use-of-Transmission-Electron-Microscopy

 Use of Transmission Electron MicroscopyOverviewA protocol describing the use of Zeiss EM9-S transmission electron microscopy is presented. MaterialZe

Use-of-the-GUS-Reporter-Gene

One of the most important considerations in the expression of heterologous proteins in plants is the choice of promoter. The study of promoter act

Basic-Methods-of-Culturing-Drosophila

实验概要Basic Methods of Culturing Drosophila实验步骤Stockkeeping1. Mechanics        Most stocks can be successfully cultured by periodic mass transfer of a

Basic-Protein-Chemistry-Techniques

Coomassie Blue Stain:  (for gels) 1) Combine 225 ml Methanol with 225 ml ddH2O. 2) Add 0.5 grams of Coomassie Blue. 3) Just before use, add 50 ml acet

学术论文常用句型汇总-再也不愁SCI了!

Beginning   1. In this paper, we focus on the need for   2. This paper proceeds as follow.   3. The structure of the paper is as follows.   4. In this

筛分仪-AS-200-basic简介

德国RETSCH(莱驰)的AS200系列分析筛分仪广泛应用于科研与开发,原材料、半成品和成品的质量控制及生产监控等领域。其可控电磁驱动能为每一产品提供最优的配置。尖锐样品也能在短时间内过筛。AS200basic具有RETSCH 产品一贯的可靠性和质量保证。该机型采用模拟制式下的振幅和筛分时间设置。所

Creation-and-Use-of-Infectious-Virus-Vector

Creation and use of your infectious vector:Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 ml of media. (This can be scaled up if desired).The f

Basic-Method-for-Indirect-Immunofluorescence-Labeling

Basic Method for Indirect Immunofluorescence LabelingBackgroundThis is the method for indirect immunofluorescence labeling; that is, the antibodies do

CREATION-AND-USE-OF-YOUR-INFECTIOUS-VECTOR

实验概要CREATION AND USE OF YOUR INFECTIOUS VECTOR实验步骤Day 1        1. Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 mL of media. (This can be scal

Basic-Fluorescent-in-situ-Hybridization-(FISH)

实验概要Fluorescence  in situ hybridization method is a kind of physical map drawing method,  use fluorescent element mark probe, to detect probe and spli

Use-an-IgG-antiIgM...

实验概要This method involves coupling an IgG anti-IgM antibody to protein A or G beads (see method below). These beads can then be used in the normal

Basic-procedures-for-bacteria-culture2

E. Elution of DNA fragments from agaroseDNA fragments are eluted from low-melting temperature agarose gels using an unpublished procedure first develo

Guidelines-for-the-Use-of-Analgesics-and-Tranquilizers-in-Laboratory-Animal

What is Anesthesia? Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are analgesia (pain relief), amn

Basic-procedures-for-bacteria-culture1

A. Phenol extraction of DNA samplesPhenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated

HOW-TO-USE-THE-COULTER-COUNTER-TO-COUNT-CELLS

1) Turn on the counter by pulling out the on/off button. You need to do this at least 10 min before use to obtain sufficient vacuum.Usually put 0.2 ml