CD133isolation,expansion,anddifferentiation
1. Renal progenitor cells were obtained from the normal portion of cortex obtained from surgically removed kidneys. 2. After dissection and passage through a graded series of meshes, CD133+ cells were isolated from the tubular fraction by magnetic cell sorting, using the MACS system.3. CD133+ cells were plated onto fibronectin in the presence of an expansion medium, consisting of 60% DMEM LG, 40% M......阅读全文
细胞分离技术（Cell－Isolation）
一、离心技术离心是研究如细胞核、线粒体、高尔基体、溶酶体和微体，以及各种大分子基本手段。一般认为，转速为10~25Kr/min的离心机称为高速离心机；转速超过25Kr/min，离心力大于89Kｇ者称为超速离心机。目前超速离心机的最高转速可达100Kr/min，离心力超过500Kg。（一）、差速离心（
Isolation－and－growth－of－mouse－primary－myoblasts
Isolation of limb muscle from neonatal mice1. Neonatal mice by decapitation or CO2 inhalation.2. Rinse the limbs with 70% ethanol and remove them wi
Isolation－of－human－endometrial－epithelial－cells
Tissue collection1. Endometrial biopsies were collected from women undergoing gynaecological procedures for benign conditions. 2. All women reported
Isolation－and－culture－of－smooth－muscle－cell
1.Male Wistar rats of about 250g body wt were anesthetized with ether and were decapitated. 2.Kidneys and thoracic aorta were removed, rinsed with
Isolation－of－genomic－DNA－from－bacteria
Note: This procedure does not work well with Gram + cocci.Transfer 1.5 mL overnight culture to a 1.5 mL microfuge tube, centrifuge for 30 sec, decant
Isolation－of－Human－Pulmonary－Epithelial－Cells
Isolation of Human Pulmonary Epithelial Cells 1. Pulmonary epithelial cells from human lung tissue were isolated.2. Lungs were perfused with 10
Primary－cardiac－fibroblast－and－cardiomyocyte－isolation
1. Ventricles were removed under sterile conditions. 2. Ventricles were placed in cold sterile primary cell culture medium, minced into approximat
Isolation－of－Nonmuscle－Actin
General Preparation1. Prepare buffers and have them cold. Make 50X stock of Buffer G and dilute as needed from frozen aliquots of 50X Buffer G.1X Extr
Isolation－of－kidney－glomeruli－from－mice
Isolation of mice glomeruli1. Mice were anesthetized by an intraperitoneal injection of Avertin (2,2,2-tribromoethyl and tertiary amyl alcohol; 17
Isolation－of－dermal－mast－cell－(DMC)
1. Human DMC were isolated from skin by enzymatic digestion.2. Dermal tissue was obtained from patients with skin after informed consent was given.3.
Isolation－of－rat－cardiac－fibroblasts－and－cardiomyocytes
1. Hearts were removed from newborn rats (day 0), put into calcium- and bicarbonate-free HEPES-buffered Hanks’ medium, cut into pieces and digeste
ISOLATION－OF－PRIMARY－MOUSE－EMBRYO－FIBROBLASTS
You will need:13.5 day pregnant mouse (we use MTK NEO inbred white mice)2 sets sterile instrumentsone containing a pair of curved forceps and a pair o
Isolation－of－human－prostatic－epithelial－cells
1. A small piece of tissue from each specimen was removed and minced. 2. The tissue was digested with collagenase overnight. 3. To remove the collagen
Nucleolar－Isolation－Protocol2
7. Resuspend the pellet with 3 ml S1 solution (Figure 3). The pellet should be resuspended readily by pipetting up and down. A pellet that cannot be r
Follicular－thyroid－cell－isolation－and－culture
Thyroid epithelial cells (also called follicular cells or principal cells) are cells of thyroid gland that are responsible for the production and
Isolation－of－normal－mammary－epithelial－cells
1. A small piece of tissue from each specimen was removed and minced. 2. The tissue was digested with collagenase overnight. 3. To remove the collagen
Human－B－cell－isolation－and－culture
实验概要This protocol provides a general protocol for human B cell isolation and culture.实验步骤B cell isolation1. Donor blood was obtained with informed con
Isolation－of－human－atrial－or－ventricular－cells
1. Human tissue was derived from atrial or ventricular biopsy specimens belonging to patients undergoing heart surgery.2. Isolated myocardial tissue w
Isolation－of－microRNA－(miRNA)
实验概要 This protocol utilizes the powerful guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of t
Primary－brain－cell－isolation－and－culture
1. Cerebella were removed from 7-day-old mice and passed through Nitex nylon netting (80 μm pore size) into primary cell system containing 20% (v/
Isolation－of－human－corneal－endothelial－cells－(HCECs)
Isolation of HCECs1. The corneoscleral tissues were rinsed three times with primary cell culture system containing 50 mg/mL gentamicin and 1.25 mg
Auxin－Immunolocalization－in－Plant－Tissues
The major naturally occurring auxin, indol-3 acetic acid (IAA), coordinates many growth and differentiation processes by modulating gene expressio
胚胎干细胞和成体干细胞标志物
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
Isolation－of－human－multipotent－mesenchymal－stem－cells－from－second
Isolation of human multipotent mesenchymal stem cells from second‐trimester amniotic fluid Culture of MSC from amniotic fluid1. Twenty amniotic flui
同济大学毛志勇教授发表Cell－Death－－Differentiation文章
基因组稳定性下降是生物体衰老发生极其重要的一个标志。细胞长期在各种因素的影响下，DNA遭受着多种损伤，若这些损伤不被及时准确地修复将诱发基因组稳定性的下降，进而影响细胞的正常生命活动。这些损伤中，DNA双链断裂（DSBs）是最为严重的基因组损伤之一。近年来，虽然关于DNA DSBs修复与衰老发
Isolation－and－characterization－of－rat－glomerular－endothelial－cells
Glomerular endothelial cells (GECs) from the kidney are in close juxtaposition to other cell types, such as mesangial cells, and may respond to as
Isolation－of－human－prostatic－smooth－muscle－cells
Human prostate tissues1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of the prostate. 2.All
Isolation－of－peripheral－blood－mononuclear－cells－(PBMC)
1. Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2. PBMC were isolated by centrifugation over lymphocyte separation medium. 3
Isolation－and－Culture－of－Human－Liver－Stem－Cells
Materials and Methods1. Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy liver tissue (
Isolation－of－retinal－pigment－epithelial－cell（二）
15. This medium is replaced after 1 day with 5% serum-containing RPE medium, and subsequent changes were made every 2 to 3 days.16. After 3 to 4 weeks