RAT／MOUSEGROWTHHO...
实验概要This Rat/Mouse Growth Hormone ELISA kit is used for the non-radioactive quantification of Growth Hormone in rat or mouse serum, plasma, tissue extracts or cell culture media samples. One kit is sufficient to measure 39 unknown samples in duplicate. This kit is for Research Use Only. Not for Use in Diagnostic Procedures. 实验原理This assay is a Sandwich ELISA ......阅读全文
RNAse－A－Treatment－of－Mouse－Cells
IntroductionRNAse A treatment of permeabilized cells followed by immunostaining is a method which allows to show if the localization of a protein into
常用的βactin－引物序列
human actin f ctc cat cct ggc ctc gct gt human actin r gct gtc acc ttc acc gtt cc product size:268 rabbit actin r agt gcg acg tgg aca tcc g rabbit act
常用的βactin－引物序列
human actin f ctc cat cct ggc ctc gct gt human actin r gct gtc acc ttc acc gtt cc product size:268 rabbit actin r agt gcg acg tgg aca tcc g rabbit act
Isolation－and－growth－of－pulmonary－artery－adventitial－fibroblasts
1. Adventitia from the main pulmonary artery was harvested neonatal calves. 2. Tissue was collected, carefully dissected free of blood vessels and f
Preparation－of－Rat－Liver－Cell－Cytosol
These protocols should yield enough cytosol and organelles for 1-200 MT/Organelle motility assays.Solutions and Reagents Freshly removed or flash fro
Preparation－of－Mitochondria－from－Rat－Liver
Preparation of Mitochondria from Rat LiverRat liver is an ideal source for functional intact mitochondria for a number of reasons. We use Sprague-Dawl
Histopathological－Approach－to－Rat－Liver－Tissue
ProcedureAfter deep ether anesthesia, dissect the rat’s liver (Wistar albino rats, 200 – 250 g) by cutting on the ventral side.Fix 2 – 3 mm. of the li
Preparing－Mitochondria－from－Rat－Liver
Liver is a convenient source for functional intact mitochondria for a number of reasons. Animal tissue is more readily homogenized than plant tissue b
Isolation－of－rat－cardiac－fibroblasts－and－cardiomyocytes
1. Hearts were removed from newborn rats (day 0), put into calcium- and bicarbonate-free HEPES-buffered Hanks’ medium, cut into pieces and digeste
Rat－urinary－bladder－urothelial－cells
1. Bladders were excised from deeply anesthetized (urethane, 1.2 gm • kg−1, i.p.) Sprague Dawley rats (of either sex), cut open, and gently stretc
ISOLATION－OF－PRIMARY－MOUSE－EMBRYO－FIBROBLASTS
You will need:13.5 day pregnant mouse (we use MTK NEO inbred white mice)2 sets sterile instrumentsone containing a pair of curved forceps and a pair o
Rodent－Retinal－Ganglion－Cell－Cultures
实验概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
Isolation－and－characterization－of－rat－glomerular－endothelial－cells
Glomerular endothelial cells (GECs) from the kidney are in close juxtaposition to other cell types, such as mesangial cells, and may respond to as
Culturing－Rat－Fetal－Neural－Stem－Cells
实验概要Rat neural stem cells (NSCs) serve as a well-established model for investigating human brain development, disease processes, and treatment stra
Purification－of－Lin,－ckit+,－Sca1+－bone－marrow－cells－for－Culture
MATERIALSMedia:Heat inactivated FBS (56°C x 30 min)PBS + 2% heat-inactivated FBSIMDM + 20% heat-inactivated FBSViral transduction: Iscove's + 20%
AbC™－AntiMouse－Bead－Kit
实验概要The AbC™ Anti-Mouse Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when u
Mouse－B－cell－isolation－with－magnetic－beads
实验概要Isolate B cells from total mouse cells实验步骤 Praparation:1. Enough MACS; put RPMI into water bath.2. Put some MACS in 15 ml tube or 1.5
Blood－collection－and－administration－of－fluids－and－drugs－(mouse)
Administration of Fluids and Drugs (Overview):Gastro-Intestinal Tract:Oral or per os (po)- - through the mouthGavage - - into the stomach via a feedin
AbC™－AntiMouse－Bead－Kit
实验概要The AbC™ Anti-Mouse Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when u
Th9－Polarization－of－Mouse－Splenocytes
实验概要Th9 cells are a subpopulation of T-helper cells, characterized to be involved in allergic diseases and resistance against intestinal nematodes.
五种免疫球蛋白（IgG、IgA、IgM、IgD、IgE）的区别与应用（二）
【IgA临床意义】 增高：与严重感染和自身免疫疾病有关，特别是肝的炎症过程会使血清IgA水平升高； 降低：发生在原发性及继发性免疫缺陷综合症中，蛋白质从肠内流失及通过被烫伤的皮肤而流失也可引起IgA浓度降低。 IgE：一种分泌型免疫球蛋白，是引起I型
Method:－Reactivating－Cell－Lines－and－Cell－Growth－for－DNA－Preparation
Purpose:Cell lines are reactivated and grown to a count of 1 x 108 cells. The cells are pelleted and stored frozen at -80 degrees C prior to DNA extra
流式应用精选——微核检测（体内体外）
注：点击文章名称查看详情。文章名称样本仪器型号备注Assessment of the Genotoxic Potential of Azidothymidine in the Comet,Micronucleus, and Pig-a AssayBone Marrow and Peripheral
AbC™－AntiRat/Hamster－Bead－Kit
实验概要The AbC™ anti-Rat/Hamster Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation
Isolation－and－culture－of－rat－coronary－microvascular－endothelial－cells
CMVE were isolated from Wistar rat hearts by digestion of primary cell isolation kit.1. Hearts mounted on a Langendorff apparatus were perfused at 3
AbC™－AntiRat/Hamster－Bead－Kit
实验概要The AbC™ anti-Rat/Hamster Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation
流式细胞术单抗直接荧光需要几种对照？
首先确认你用的直标抗体的荧光染料是什么，再确定该抗体的来源种属，选择相应的同型对照。如：你现在想检测小鼠淋巴细胞表面的CD4抗原， 荧光染料为FITC， 抗体来源为大鼠的IgG1亚型， 即Rat anti Mouse CD4-FITC （IgG1）， 你所需要的同型对照就应该是Rat IgG
Murine－Skin－Transplantation
Prior to the initiation of experiment:Obtain IACUC approvalSterilize surgical instruments and Q-tipsI. Harvest Donor TissueEuthanize donor mouse (in a
Mouse－Eotaxin－2－ELISA－Kit使用原理
Mouse Eotaxin 2 ELISA Kit使用原理小鼠嗜酸粒细胞趋化蛋白Eotaxin2(Eotaxin2/CCL24)ELISA试剂盒货号:FK-QZ3338规格：96T/48TMouse Eotaxin 2 ELISA Kit使用原理The kit was a solid phase
Mouse－Desmin,Des－ELISA－Kit使用原理
Mouse Desmin,Des ELISA Kit使用原理适应物种： Human,Rat,Mouse,Rabbit应用： 科研实验检测方法： 酶联检测检测限： 科研数量： 100规格： 96T/48TMouse Desmin,Des ELISA Kit使用原理小