Polygalacturonaseassay
This enzyme is famous for being involved in the development of the GMO tomatoes (more information from the link at the foot of this page). The cells of these tomatoes have been genetically modified to contain a reversed copy of the gene for this enzyme. This produces anti-sense mRNA which combines with the normal mRNA for polygalacturonase, effectively supressing its synthesis. As a result the GM tomatoes have o......阅读全文
Pectinase assay
Pectinases are actually a mixture of enzymes, which, along with others such as cellulase, are widely used in the fruit juice industry where they are
Protease assay
In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to soft
Protease assay
实验概要 In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part
DGK Assay
Buffers: - 2X buffer 10 ml 0.5 M imidazol, pH 6.6 0.21 g LiCl 1.25 ml 1 M MgCl2 1.0 ml 0.1 M EGTA, pH 6.6 --> Bring volume up to 50 ml with distille
Motility Assay
DescriptionVarious phenotypic characteristics are requiredfor a cancer cell to successfully complete the metastaticcascade. Among these, acquisition o
Phosphate Assay
1. Make standards using sodium phosphate at the following uM concentrations: 0, 2, 5, 7, 10, 20, 40, 60, and 80. Use the screw top glass tubes.2. Dry
TUNEL assay
PROTOCOL:•Deparaffinize and rehydrate slides:3 x 3´ Xylene3 x 2´ 100% ethanol1 x 2´ 95%, 80%, 70% ethanol (each)1 x 5´ 1x PBS•Microwave antigen retrie
Polygalacturonase assay
This enzyme is famous for being involved in the development of the GMO tomatoes (more information from the link at the foot of this page). The cells o
Aspartate Assay
实验概要The Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is converted
Bradford Assay
The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue