IPAC2012ConferenceOrganization
The International Process Analysis & Control Congress, China 2012 (IPAC 2012) is the first event sponsored by China Instrument and Control Society (CIS). IPAC 2012 is a professional conference which takes Process Analysis，Control and related technology as its core. Sponsored by: China Instrument and Control Society (CIS) The undertaker： East China University of Technology Zhejiang University g......阅读全文
International－Process－Analytics－and－Control－Congress
International Process Analysis and Control Congress, China 2012IPAC 2012 Call for paper and Introduction The International Process Analysis & Control
FTNIR－Process－Monitoring
FT-NIR Process MonitoringToday many manufacturers are striving not only to produce the highest quality final product but also to improve manufactu
Glycosphingolipid－analysis
1) Incubate cells with 1 µCi/ml of 3H-galactose for 72 hours.---> If treatment is for an extended period of time: treat in serum free media containing
Lipid－analysis
Thin layer chromatography is based on the separation of a mixture of compounds as it migrates with the help of a suitable solvent through a thin layer
WHITE－PAPER:－Virotherapy－Process－Optimization（一）
WHITE PAPER: Virotherapy Process Optimization采用一系列方法将病毒工程开发用于疾病治疗已经成为一种新型的应用，该应用广泛的定义为病毒疗法，包括用于基因治疗的病毒载体(Figure 1)和具有溶瘤细胞功能的病毒(Figure 2)。虽然这些产品大部分
Detection－of－apoptotic－process－in－situ－using－immunocytochemical
1. INTRODUCTION Apoptosis was observed from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pathological
WHITE－PAPER:－Virotherapy－Process－Optimization（二）
Figure 3. Virus Counter 3100 (top) and Autosampler (bottom)Proof of Principle病毒计数仪已经被广泛应用于多种病毒的计数，包括基因传递载体和溶瘤功能的病毒。这里我们列出一些检测的数据，证实病毒计数仪快速的病毒定量在病毒疗法领域
Analysis－of－Heme－and－Hemoproteins
Heme is perhaps the most ubiquitous cofactor found in nature and the most functionally diverse. Hemoproteins are involved in cell respiration (cyt
Flow－Cytometry－Analysis
PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide r
Lineage－Analysis－of－Blood
Materials:Capillary tubes1.5 mL Eppendorf microfuge tubes15 mL conical centrifuge tubes96-well V-bottom plates (Corning Costar 3894, from Fisher)Flow
Biosynthesis－and－Analysis－of－Bilins
The term bilin is a collective one to describe a broad group of open chain tetrapyrroles and derives from the name “bile pigments” as the first of
Analysis－of－Oligosaccharide－Ligands
Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity ChromatographyAnalysis of Oligosaccharide Ligands by High Performance Liquid A
CELL－CYCLE－ANALYSIS
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isol
Analysis－of－murine－BM
Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis. Techniques to do this are well established in huma
Leister－Process－Tech.收购IR－Microsystems
Leister Process Technologies宣布收购瑞士传感器公司IR Microsystems SA，该公司是用于气体检测单元的二极管激发器的优秀供应商。此次收购在Leister公司长期发展策略上是一个重要的里程碑，公司的长期发展战略集中在传感器、光学和激光技术。 “以IR M
Analysis－and－Reconstitution－of－Phycobiliproteins:－Methods－for－the－...
Analysis and Reconstitution of Phycobiliproteins: Methods for the Characterization of Bilin Attachment ReactionsPhycobiliproteins are a homologous fam
Molecular－Analysis－and－Results－－DNA
Theory of CGHComparative genomic hybridization (CGH) is a fairly new molecular cytogenetic technique that allows detection of DNA sequence copy number
FACS－Analysis－of－ES－Cells
Isolate cells and dissociate to single cell suspension (can use Gibco Cell Dissociation Buffer, Accutase or Trypsin)Wash with 10% FBS/DMEM:F12For surf
Protocols－for－LCM－preparation－and－analysis
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC. StainingII. Pr
Genomic－Southern－Blot－Analysis
This chapter describes a detailed protocol for genomic Southern blot analysis which can be used to detect transgene or endogenous gene sequences i
PAM:－Prediction－Analysis－for－Microarrays
PAM: Prediction Analysis for MicroarraysClass Prediction and Survival Analysis for Genomic Expression Data Mining Features:Performs sample classificat
Microtubule－Spindowns－for－Visual－Analysis
Microtubule spindowns for visual analysis can be performed on single microtubules or microtubules nucleated from axonemes/centrosomes. Although live D
Simultaneous－analysis－of－DNA－content
Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques. William Telford. Louis E. King and Pamela
New－white－paper－explains－how－to－increase－brewing－process－……
Ensuring production processes will operate as necessary and generate the desired product quality and yield in the required time is central to succ
Detection－of－apoptotic－process－in－situ－using－immunocytochemical2
B) TUNEL in situ procedureB.2.1 Materialsproteinase K (pK) (A2), H2O2 , TdT buffer (A1), TdT enzyme (A2), biotinylated dUTP (A2), TB buffer (A1), seru
SOLID－TUMOR－CULTURES－FOR－CHROMOSOME－ANALYSIS
I. Purpose:A. Samples of solid tumors or lymph nodes may be sent from patients with cancer. These samples should be processed directly and also set up
TISSUE－FIBROBLAST－CULTURES－FOR－CHROMOSOME－ANALYSIS
I. Purpose:A: Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspected
Flow－Cytometric－Analysis－of－Cell－Cycle
Fixation1) Collect 2 X 106 cells.2) Pellet cells by spinning at 1,000 rpm, 4°C for 5 minutes.3) Resuspend cell pellet in 1 ml of cold PBS.4) Fix cells
TISSUE－FIBROBLAST－CULTURES－FOR－CHROMOSOME－ANALYSIS
实验概要 Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspect
Lipoprotein－Analysis－Week－2:－Electrophoresis
Lipoprotein Analysis Week 2: Electrophoresis IntroductionSDS polyacrylamide gel electrophoresis (SDS PAGE) will be used to assess the purification pr