Centrifugation
CentrifugationThe single most important advance in the use of centrifugal force to separate biologically important substances. was the coupling of mechanics, optics and mathematics by T. Svedberg and J.W. Williams in the 1920's. They initiated the mathematics and advanced the instrumentation. 1 to a point where it was possible to prove that proteins were large molecules that could be weighed in a centri......阅读全文
Centrifugation
CentrifugationThe single most important advance in the use of centrifugal force to separate biologically important substances. was the coupling of mec
Centrifugation－Theory
Centrifugation is a process used to separate or concentrate materials suspended in a liquid medium. The theoretical basis of this technique is the eff
Appendix－F:－Centrifugation
The single most important advance in the use of centrifugal force to separate biologically important substances. was the coupling of mechanics, optics
Centrifugation2
RELATIVE CENTRIFUGAL FORCE Figure F.2. Nomogram for R.C.F.Modern day ultracentrifuges can generate forces in excess of 300,000 times that of gravity,
Appendix－F:－Centrifugation——2
ROTOR COMPARISONSWhen separating a particle, it is convenient to be able to compare one rotor's design characteristics with those of a differing r
离心技术（centrifugation－technique）与离心机类型(1)
最大速度方法(1)移动界面超速离心法含几个组分的样品在足够高的离心场中离心时，每种颗粒都达到其最大沉降速度，这时样品开始分离。离心管的上层逐渐形成透明的上清液，并形成对应于样品各组分的一系列浓度界面，界面的移动相对于每种组分来说是特征的。虽然利用这种方法不一定能实现组分的纯化分离，但可以通过监测界面
离心技术（centrifugation－technique）与离心机类型(2)
离心机类型通常所使用的离心机根据转子转速大小的不同可分为普通离心机、高速离心机和超速离心机三类。(1)普通离心机一般来说，最大转速不超过6000r/min 者属普通离心机；如国产的80—1型，LXJ—Ⅱ型等。离心机转速与相对离心力的测算，如图2—37。普通离心机转子在室温下运转，转子室内的温度一般无
谈谈实验室离心机高速离心的工作原理和特点
通常情况下，制备型超高速（preparative super-speed）实验室离心机高速离心的原理有差速离心（differential centrifugation）和密度梯度离心（density gradient centrifugation）两种。其中，差速离心法是相对更为常用的方法。Usua
离心的基础知识
Centrifugation is a process used to separate or concentrate materials suspended in a liquid medium. The theoretical basis of this technique is the eff
区带离心的概念和用途
狭义的区带离心（zonal centrifugation）主要是采用蔗糖，偶尔也用甘油或其他溶液铺梯度，因此也被称作蔗糖梯度离心（sucrose graduation centrifugation）。而平衡离心（equilibrium centrifugation）是在密度梯度中进行，以CsCl制成
Plasma－and－Serum－Preparation
实验概要Serum is the liquid fraction of whole blood that is collected after the blood is allowed to clot. The clot is removed by centrifugation and the
Isolation－of－liver－lymphocyte
Isolation of liver lymphocyte Several lymphocyte subpopulations reside in the normal adult human liver. Liver lymphocytes mainly include a large n
Extraction－of－DNA－From－Plants－Using－Plant－DNAzol®－Reagent
实验概要Plant DNAzol® is an extra-strength-DNAzol® reagent (patent pending) specifically formulated for the isolation of genomic DNA from plants. The Plan
Large－Scale－Plasmid,－Cosmid,－BAC,－PAC,－and－Fosmid－DNA－Isolation
DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation Version 3b - updated September 26, 1999The Most Recent Roe Lab Imple
重组DNA的分离、克隆与测序实验手册7
III. Methods for DNA isolationA. Large scale double-stranded DNA isolationThe method used for the isolation of large scale cosmid and plasmid DNA is a
Embryo－Lysates－－Immunoprecipitation
Embryo lysatesTake 25 embryos and place into 1.7ml centrifuge tube.Rinse once in lysis buffer (add ~ 1ml) and remove by aspirationAdd 500 µL lysis buf
Transformation－of－E.－coli－by－Electroporation
实验概要 Electrocompetent bacteria are prepared by growing cultures to mid-log phase, washing the bacteria extensively at low temperature, and
Measurement－of－GFP－Expression－and－DNA－Content－in－Permeabilized－Cells
ReagentsCells to be studied expressing green fluorescent protein (GFP). Note that the same cell type without GFP is needed as a control.1 X PBS2% Buff
Preparation－of－Plasmid－DNA－by－Alkaline－Lysis－with－SDS:－Maxipreparation
实验概要Plasmid DNA is isolated from large-scale (500 ml) bacterial cultures by treatment with alkali and SDS.主要试剂Buffers and SolutionsAlkaline lysis solu
ImmunohistochemistyFluorescence－Protocol1
MaterialsCytokine-specific Primary Antibodiesunlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF'&
Lyophilizing－Cells
Inoculate 200 ml L-broth supplemented with appropriate antibiotics with the bacteria to be lyophilized.Incubate the culture at 37°C with vigorous shak
Lyophilizing－Cells
Lyophilizing CellsInoculate 200 ml L-broth supplemented with appropriate antibiotics with the bacteria to be lyophilized.Incubate the culture at 37°C
DNA提取中EB的去除实验方法
Removal of Ethidium Bromide from DNA by Extraction with Organic SolventsJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourn
DNA纯化手册2
Key points to observe: a. Use a endA1- E. coli strain for plasmid propagation and isolation whenever possible. The instability of plasmids isolated fr
巨噬细胞和单核白细胞
· Lymphocyte Transformation (Donis-Keller lab)Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocytes) from antico
细胞结构成分的离心分离技术1
就像可以把细胞从组织中分离出来进行研究一样，人们也可以把细胞器从细胞中分离纯化出来，以研究它们各自特有的的化学组成、酶活性和代谢特点。尽管在一个世纪前就有人试图分离细胞器，但直到20世纪40年代有了超速离心机和细胞匀浆技术后，才真正建立了细胞器的分离技术。用这一技术可以获得相对纯净的各种细胞器和大分
Maxiprep－preparation－of－Plasmid－DNA
实验概要The PureLink™ HiPure Plasmid DNA Maxiprep Kit allows purification of 500–850 μg of high-quality plasmid DNA from 100–200 mL overnight E. coli cu
Thawing－－Incubating－Human－－Animal－Liver－Microsomes
实验概要BackgroundThe liver is the major organ for metabolism of endogenous substrates as well as exogenous drugs. There are several in vitro tools avai
密度梯度离心基础知识（五）
序数符号表示为： ①细胞核 ②质膜 ③细胞质 ④细胞核 ⑤线粒体 ⑥溶酶体 ⑦线粒体 ⑧溶酶体 ⑨内质纲 ⑩病毒(1)细胞核 (2)质膜 (3)细胞质 (4)细胞核 (5)线粒体 (6)溶酶体 (7)线粒体 (8)溶酶体 (9)内质网(10）病毒 (11)核蛋白体 (12)核糖体 (13)D
Isolation－of－peripheral－blood－mononuclear－cells－(PBMC)
1. Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2. PBMC were isolated by centrifugation over lymphocyte separation medium. 3