Centrifugation
CentrifugationThe single most important advance in the use of centrifugal force to separate biologically important substances. was the coupling of mechanics, optics and mathematics by T. Svedberg and J.W. Williams in the 1920's. They initiated the mathematics and advanced the instrumentation. 1 to a point where it was possible to prove that proteins were large molecules that could be weighed in a centri......阅读全文
重组DNA的分离、克隆与测序实验手册8
B. Midiprep double-stranded DNA isolationA midi-prep double-stranded DNA isolation has been developed to generate a sufficient amount of template DNA
E.Z.N.A.™－Fastfilter－Plasmid－Mega－Protocol
实验概要The E.Z.N.A.™ family of products is an innovative system that radically simplifies extraction and purification of nucleic acids from a variety
BAC－DNA分离方法－Isolation－of－BAC－DNA－from－Largescale－Cultures
Isolation of BAC DNA from Large-scale Cultures Joseph Sambrook Peter Maccallum Cancer Institute and The University of Melbourne, Austral
Erythrophagocytosis
OUTLINE The erythrophagocytosis assay is performed to compare the phagocytic rate with and without anti-eythrocyte antibody in either macrophages fr
Probiotic－Attributes－of－Lactic－Acid－Bacteria－Isolates
IntroductionPresently the consumption of probiotic food is highly popular worldwide for their health beneficial effects. Commonly used probiotic bacte
Separation－of－Platelets－from－Whole－Blood
Purpose This protocol describes how to isolate human platelets from whole blood. Isolated platelets are used for static adhesion assays, for flow ch
Preparation－of－tubulin2
DAY 2: Cycling preparation of MT protein. Keep the brains in an evacuated plastic ziplock bag buried in ice from the time of slaughter during tran
Preparation－of－human－platelets
Preparation of human platelets 1. Human blood was taken from drug-free volunteers on the day of the experiment using acidic citrate dextrose
Midiprep－preparation－of－Plasmid－DNA
实验概要The PureLink™ HiPure Plasmid DNA Midiprep Kit allows purification of 100–350 μg of high-quality plasmid DNA from 15–25 mL overnight E. coli cul
速率区带离心法－|你的分离纯化方法选对了吗？
密度梯度离心（isodensity centrifugation method）又称为区带离心。该方法的优点是可以同时使样品中几种或全部组分分离，具有良好的分辨率，分离效果好，可一次获得较纯组分。缺点是离心时间较长，需制备梯度液，操作要求高。按照离心分离原理，密度梯度离心又可分为速率区带离心法（ra
细胞组分和细胞器——细胞膜
Isoelectric Focussing of Membrane Protein by Slab Gel Method (Hancock Lab) Isolation of Outer Membrane Protein from P.Aeruginosa with Octyl-Poe (Hanc
Extraction－of－DNA－using－DNAzol®－Reagent
实验概要DNAzol® Reagent (Genomic DNA Isolation Reagent) is a complete and ready-to-use reagent for the isolation of genomic DNA from solid and liquid sa
Protocols－for－the－preparation－of－tumour－cells－for－s.c.－injections－in－mice
For the establishment of solid tumour in nude mice, tumour cells are treated in a sterile environment under a cell culturing hood. Disposable sterile
Protocols－for－the－preparation－of－tumour－cells－for－s.c.－injections－in－mice
For the establishment of solid tumour in nude mice, tumour cells are treated in a sterile environment under a cell culturing hood. Disposable sterile
RNA－Isolation－Protocol
Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109 cells (OD600= 0.2 Dilute cells or scale up)Pipet 30 ml of RNAProtect Bacteria Reagen
Subculture－of－Suspension－Cell－Lines
Aim In general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transfo
细胞膜蛋白质提取方法
NRC Institute for Biological SciencesTriton X-114 extraction protocol (Hydrophobic protein preparation)Ressuspend cells in Solution A (dil 1/8) and ad
离心机5000转/10分钟会使细胞破碎吗
建议读下”Evaluation of Centrifugation Technique and Effect of Epinephrine on Fat Cell Viability in Autologous Fat Injection”这篇文献，最好不要超过5000RPM，一般来讲1000RPM
RNA－Isolation－Protocol
RNA Isolation Protocol(Revised 5-15-2003)Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109 cells (OD600= 0.2 Dilute cells or scale up)
速度离心的概念
中文名称速度离心英文名称velocity centrifugation定 义根据被分离物质的体积差异在一定离心速度下沉降速度不同而分离的方法。应用学科细胞生物学（一级学科），细胞生物学技术（二级学科）
速度离心法的技术特点
中文名称速度离心英文名称velocity centrifugation定 义根据被分离物质的体积差异在一定离心速度下沉降速度不同而分离的方法。应用学科细胞生物学（一级学科），细胞生物学技术（二级学科）
Culturing－Human－Neural－Stem－Cells
实验概要 Neural stem cells (NSC) are valuable resources because of their ability to differentiate into neurons and glial cells with applications in n
AntiDYKDDDDK－tag－(L5)－Affinity－Gel
实验概要Anti-DYKDDDDK tag (L5) affinity gel is a purified rat IgG2a, κ monoclonal antibody covalently attached to agarose by hydrazide linkage. It is us
重组DNA的分离、克隆与测序实验手册
edited by Bruce A. Roe Judy S. Crabtreeand Akbar S. KhanDepartment of Chemistry and Biochemistry The Uni
Estimation－of－Fat－in－Milk－Samples
实验概要 Milk fat measurement is a common task in the dairy industry, because milk fat content is one factor that determines milk price and is neces
高速离心的用途
中文名称高速离心英文名称high speed centrifugation定 义一般指离心速度在18 000～35 000 r/min、离心力在60 000～100 000×g 范围的离心。应用学科生物化学与分子生物学（一级学科），方法与技术（二级学科）
高速离心的概念
中文名称高速离心英文名称high speed centrifugation定 义一般指离心速度在18 000～35 000 r/min、离心力在60 000～100 000×g 范围的离心。应用学科生物化学与分子生物学（一级学科），方法与技术（二级学科）
梯度离心的定义
中文名称梯度离心英文名称gradient centrifugation定 义在离心力场作用下样品中各组分会按照各自的特性沉降或上浮到梯度层相应的位置而被分离。应用学科生物化学与分子生物学（一级学科），方法与技术（二级学科）
低速离心的定义
中文名称低速离心英文名称low speed centrifugation定 义转速为8000 r/min以下，相对离心力为10 000×g以下的离心。主要用于分离细胞、细胞碎片及培养基残渣等颗粒物。应用学科生物化学与分子生物学（一级学科），方法与技术（二级学科）
低速离心的概念
中文名称低速离心英文名称low speed centrifugation定 义转速为8000 r/min以下，相对离心力为10 000×g以下的离心。主要用于分离细胞、细胞碎片及培养基残渣等颗粒物。应用学科生物化学与分子生物学（一级学科），方法与技术（二级学科）