PhosphoaminoacidanalysisofnonTCAprecipitableproteins

Protocol－for－Trichloroacetic－Acid－(TCA)－Precipitation

IntroductionThe efficiency of nucleotide incorporation in DNA/RNA polymerization reactions (e.g. transcription, reverse transcription, and DNA replica

Acid－Phenol－Yeast－RNA－Prep

This is the preferred method for yeast RNA preparationuse Gloves and RNAse free solutions throughout.1. Use a YPD overnight culture to innoculate fres

核酸提取(The－Extraction－of－Nucleic－Acid)

【实验目的】 1．掌握核酸提取的基本原理和基本方法 2．掌握检测核酸浓度及纯度的原理及方法   【实验原理】 DNA是遗传信息的载体,是最重要的生物信息分子,是分子生物学研究的主要对象,因此DNA的提取也应是分子生物学实验技术中最重要、最基本的操作。如不能有效的完成DNA提取方面的工作，那就根本谈不

Crystallization－of－Kinesin－Family－Motor－Proteins

Motor proteins of several kinesin family groups have now been crystallized: monomeric Kinesin-1 motor domains from human, rat andNeurospora (Kull et a

Acetylation－(or－Succinylation)－of－Amino－Groups－on－Proteins

Acetylation (or Succinylation) of Amino Groups on ProteinsREFERENCE: Hanock and Benz. 1986. BBA. 860:699-707.PURPOSE: Derivitization of amino groups t

Phosphoproteins－pr...

实验概要The following procedure provides a method of detection of phosphorylated proteins.实验步骤1. To a sample of protein solution containing 1-100 ng of

Genomic－Southern－Blot－Analysis

This chapter describes a detailed protocol for genomic Southern blot analysis which can be used to detect transgene or endogenous gene sequences i

Analysis－and－Reconstitution－of－Phycobiliproteins:－Methods－for－the－...

Analysis and Reconstitution of Phycobiliproteins: Methods for the Characterization of Bilin Attachment ReactionsPhycobiliproteins are a homologous fam

Microtubule－Spindowns－for－Visual－Analysis

Microtubule spindowns for visual analysis can be performed on single microtubules or microtubules nucleated from axonemes/centrosomes. Although live D

PAM:－Prediction－Analysis－for－Microarrays

PAM: Prediction Analysis for MicroarraysClass Prediction and Survival Analysis for Genomic Expression Data Mining Features:Performs sample classificat

Protocols－for－LCM－preparation－and－analysis

Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC. StainingII. Pr

Simultaneous－analysis－of－DNA－content

Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques.  William Telford. Louis E. King and Pamela

Molecular－Analysis－and－Results－－DNA

Theory of CGHComparative genomic hybridization (CGH) is a fairly new molecular cytogenetic technique that allows detection of DNA sequence copy number

FACS－Analysis－of－ES－Cells

Isolate cells and dissociate to single cell suspension (can use Gibco Cell Dissociation Buffer, Accutase or Trypsin)Wash with 10% FBS/DMEM:F12For surf

Size－and－Shape－of－Protein－Molecules5

Hydrodynamic Analysis and EM Applied to Large Multisubunit ComplexesThe text box above showed the application of the Siegel–Monte analysis to SMC prot

Size－and－Shape－of－Protein－Molecules5

Hydrodynamic Analysis and EM Applied to Large Multisubunit ComplexesThe text box above showed the application of the Siegel–Monte analysis to SMC prot

Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites六

N-glycosylation modification of proteins has reported to improve the health of living organisms through antibacterial activity [68], antioxidant a

丙烯酰胺胶中肽提取和蛋白消化技术

Digestion of Proteins and Extraction of Peptides from an Acrylamide GelSherry Niessen, Ian McLeod and John R. Yates IIIDepartment of Cell Biology, The

蛋白质和多肽MALDIMS分析方法

ABSTRACTFor MALDI-TOF-MS analysis of proteins and peptides, samples are cocrystallized with an excess of organic matrix that absorbs at a specific wav

耐Hydrofluoric－acid电极使用技巧

1.将耐Hydrofluoric acid电极连接到ph计的输入端，正确连接。2. 正确配制耐Hydrofluoric acid电极浸泡混合液：取37.25克KCL和1.75克混合磷酸盐粉末配制成250ml混合溶液。3.严禁用耐Hydrofluoric acid电极测量能溶解PVC以及ABS的有机溶

Testing－the－effects－of－retinoic－acid－on－tail－formation

Probiotic－Attributes－of－Lactic－Acid－Bacteria－Isolates

IntroductionPresently the consumption of probiotic food is highly popular worldwide for their health beneficial effects. Commonly used probiotic bacte

Quantification－made－easy

With the aid of spectroscopy, the quantitative analysis of nucleic acids and proteins has established itself as a routine method in many laboratories.

分光光度计的使用

With the aid of spectroscopy, the quantitative analysis of nucleic acids and proteins has established itself as a routine method in many laboratories.

分光光度计知识

With the aid of spectroscopy, the quantitative analysis of nucleic acids and proteins has established itself as a routine method in many laboratories.

Production－of－Recombinant－Proteins－in－SuspensionCultured－Plant－Cells

Plants have emerged in the past decade as a suitable alternative to the  current production systems for recombinant pharmaceutical proteins and,  toda

BTG－family－proteins－and－cell－cycle－regulation

BTG2 is found to be one of the immediate early genes up-regulated by neural growth factor (NGF) and epidermal growth factor (EGF). Its transcriptional

Bacterial－Expression－of－GSTfusion－Proteins

1.  Grow cells in 5ml (or more) of LB-Amp overnight for a starter culture. 2.  Grow larger culture (100x volume of starter culture) using the overnigh

反向微柱的准备Preparation－of－ReversedPhase－Microcolumns

INTRODUCTIONOne versatile strategy for sample cleanup prior to MALDI-MS analysis uses microscale columns designed for direct sample elution onto the M

Flow－Cytometric－Analysis－of－Cell－Cycle

Fixation1) Collect 2 X 106 cells.2) Pellet cells by spinning at 1,000 rpm, 4°C for 5 minutes.3) Resuspend cell pellet in 1 ml of cold PBS.4) Fix cells