TimingofCycles
MaterialsMonolayer cultures grown in 75 mm culture flasks (Cells from Exercise 11.4 may be used, or cultures of tetrahymena, yeast, or algae may be used.)H-thymidine with at least 4 µc/ml, 0.36 c/mMPhosphate buffered saline (PBS), TrypsinMethanol:Acetic acid (3:1) fixativeNuclear track emulsion and equipment for autoradiographic analysisSubbed slides, coverslips, permountGiemsa stainMicroscope (Phase contrast if......阅读全文
Timing－of－Cycles
MaterialsMonolayer cultures grown in 75 mm culture flasks (Cells from Exercise 11.4 may be used, or cultures of tetrahymena, yeast, or algae may be us
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ContentsFactors Affecting the PCR Nested Primer PCRPrimer LengthDegenerate PrimersElongation Temperature and TimeReaction BufferCycle NumberDenaturin
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