StainingMethodsforcell
death Z. Xia 10/2/95The simplest way: trypan blue. Dead cells stain blueNon-fixed cells: FDA(fluorescein diacetate)-green, alive cells; P.I. (propidium iodide)-red, dead cells35 mm plates:Note:If the P.I. staining is not strong enough to be picked up easily under your scope, use 2 X P. I., i.e., 4 ul 2 mg/ml in 2 ml mediumAfter staining, need to examine the staining right away, otherwise, the green staining......阅读全文
细菌的芽孢染色(spore－staining)
实验原理细菌的芽胞具有厚而致密的壁，透性低，不易着色，若用一般染色法只能使菌体着色而芽胞不着色（芽孢呈无色透明状）。芽孢染色法就是根据芽孢既难以染色而一旦染上色后又难以脱色这一特点而设计的。所有的芽孢染色法都基于同一个原则：除了用着色力强的染料外，还需要加热，以促进芽孢着色。当染芽孢时，菌体也会着色
蛋白质检测
· Protein detection (Aberdeen's Lab)The method used to locate the proteins following 2D-PAGE depends on the nature of the original sample.
Plant－Genome－Annotation－Methods
Annotation of plant genomic sequences can be separated into structural and functional annotation. Structural annotation is the foundation of all g
Analysis－and－Reconstitution－of－Phycobiliproteins:－Methods－for－the－...
Analysis and Reconstitution of Phycobiliproteins: Methods for the Characterization of Bilin Attachment ReactionsPhycobiliproteins are a homologous fam
General－Laboratory－Methods－for－Tetrapyrroles
There are thousands of porphyrins and metalloporphyrins, and hundreds of new derivatives appear each year. This variety arises because the cyclic
Basic－Methods－of－Culturing－Drosophila
实验概要Basic Methods of Culturing Drosophila实验步骤Stockkeeping1. Mechanics Most stocks can be successfully cultured by periodic mass transfer of a
Methods－for－Xylem－Sap－Collection
Xylem and phloem are essential for the exchange of solutes and signals among organs of land plants. The synergy of both enables the transport and
Staining－Procedure－for－Flow－Cytometric－Detection－of－Human－Cyclins
Staining Procedure for Flow Cytometric Detection of Human CyclinsThis is a standard protocol used at Pharmingen for Quality Control testing of the ant
Intracellular－Immunofluorescent－Staining－for－Flow－Cytometry2
Table 2: Human Cytokines: Intracellular Staining Quick GuideHuman Cytokines: Intracellular Staining Quick GuideHuman CytokineCell SourceActivationIncu
Human－Alkaline－Phosphatase－staining－of－transgenic－embryo/tissue
AP Buffer:100 mM Tris-HCl, pH 9.5100 mM NaCl10 mM MgCl2PTM:PBS0.1% Tween-202 mM MgCl2Since this is generally done in conjunction with lacZ staining, e
Betagal－staining－of－eukaryotic－cells－in－vitro
(Modification of methods of Dr. Seong-Seng Tan and Promega's "Protocols and Applications Guide")Cells previously transfected with a lac Z construc
银染(silver－staining)操作规程
实验原理：在碱性条件下，用甲醛将蛋白带上的硝酸银（银离子）还原成金属银，以使银颗粒沉积在蛋白带上。染色的程度与蛋白中的一些特殊的基团有关，不含或者很少含半胱氨酸残基的蛋白质有时候呈负染。银染的详细机制还不是非常清楚。 试剂：乙醇、冰醋酸、乙酸钠、硫代硫酸钠、硝酸银、碳酸钠、甘氨酸或EDTA.Na
免疫细胞表面抗原分子CD家族对照表(CD1CD247)2
CD1cPositive staining (normal): subset of normal peripheral B cellsPositive staining (disease): myeloid leukemias and some B cell malignancies Negativ
Analysis－of－Proteins－using－Small－Format－2D－Gel－Electrophoresis
Preparation of protein samplesIntracellular virus proteinsThe following method has been developed principally for the analysis of intracellular protei
LIVE/DEAD®－Fixable－Dead－Cell－Stain－Kits
实验概要The LIVE/DEAD® Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These assays are
Protocol－to－Count－Cell－Number－of－Preimplantation－Embryos
Protocol to Count Cell Number of Preimplantation Embryos using Nuclear Staining with Hoechst 33342 or DAPI Introduction The following is a simple pro
免疫细胞表面抗原分子CD家族对照表(CD1CD247)
Exalpha Biologicals produces and sells products for Life Science Research, including monoclonal and polyclonal antibodies, for use in immunohistochemi
免疫细胞表面抗原分子CD家族对照表(CD1CD247)3
CD7Membrane glycoprotein and Fc receptor for IgM Homologous to TCR gamma, Ig kappaMembrane expression early during T ontogeny, before TCR rearrangemen
Combined－Flow－Cytometric－Measurement－of－Two－CellSurface－Antigens
INTRODUCTIONFlow cytometry is frequently used to assess nucleic acid content in individual cells. Based on DNA content alone, however, cells in the qu
Combined－Flow－Cytometric－Measurement－of－Two－CellSurface－Antigens2
DNA and RNA Staining6. Stain cells with 7-AAD: i. Resuspend the cells from Step 5 in 0.5 mL of NASS containing 10 µg/mL of 7-AAD. Incubatefor 20 min a
ImmunohistochemistyEnzymatic－Protocol
OverviewR&D Systems provides monoclonal, polyclonal and biotinylated antibodies for immunohistochemical use. The following protocol has been developed
LIVE/DEAD®－Fixable－Dead－Cell－Stain－Kits
实验概要The LIVE/DEAD® Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These assays are
The－TRC－shRNA－Design－Methods－and－Rules
OverviewWe design shRNA molecules with an algorithm. Our algorithm uses several criteria to rank potential 21mer targets within each human and mouse R
The－Fungal－Genetics－Stock－Center－Methods
Some basic Neurospora methods compiled at StanfordVogel's Minimal MediumD. D. Perkins - Hints for the care, feeding and breeding of NeurosporaStra
Tissue－Culture－Methods3
REFERENCES:R. Ian Freshney, Culture of Animal cells: A manual of basic techniques, Wiley-Liss, 1987.VI. TISSUE CULTURE PROCEDURESEach student should m
COMPARISION－OF－DIFFERENT－PROTEIN－DETERMINATION－METHODS
COMPARISION OF DIFFERENT PROTEIN DETERMINATION METHODSCompanyMethodDetection RangeApplications -CompatibilityAssay protocolPrecautions-InterferencesA
Tissue－Culture－Methods1
I. TYPES OF CELLS GROWN IN CULTURETissue culture is often a generic term that refers to both organ culture and cell culture and the terms are often us
《Nature－Methods》大脑地图图解
多年来，出现了多种“大脑地图”，每一种都关注不同的大脑过程，从新陈代谢到认知功能。虽然这些地图很重要，但单独使用它们会限制研究人员从中得出的发现。 现在，来自蒙特利尔神经学研究所的一个团队和其他研究人员在一个地方汇集了40多张现有的大脑地图。这个被称为神经图谱的数据库有望帮助科学家发现不同大脑
Tissue－Culture－Methods2
IV. MAINTENANCECultures should be examined daily, observing the morphology, the color of the medium and the density of the cells. A tissue culture log
Histochemical－staining－of－sea－urchin－embryos－for－(AP)－enzyme－activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity1. Obtain embryo samples, tube of AP substrate buffer and tu