MeasurementofCarbonFixationRatesinLeafSamples
Generation of a Light Curve To address the hypothesis concerning photosynthetic efficiency it is necessary to expose sun and shade leaves to a range of light intensities long enough for them to fix significant amounts of carbon. It is necessary to expose identical surface areas under favorable conditions which are identical for all leaves except for light intensity (the experimental variable). A means of ......阅读全文
The－Effect－of－Caffeine－on－the－Heart－Rates－of－ThreeDay－Old－Chick－Embryos
Objective The purpose of this experiment is to observe the effect of caffeine on the heart rate of three day old chick embryos by directly treati
ICBR－Flow－Cytometry－Core－Laboratory－Paraformaldehyde－Fixation－of－Cells
BackgroundThis fixation method is good for cells labelled by fluorochrome-conjugated antibodies to membrane antigens. It will stabilize the light scat
Flow－Cytometric－Analysis－of－Cell－Cycle
Fixation1) Collect 2 X 106 cells.2) Pellet cells by spinning at 1,000 rpm, 4°C for 5 minutes.3) Resuspend cell pellet in 1 ml of cold PBS.4) Fix cells
麦克仪器圆满参加Carbon－2011碳科学技术大会
7月29日，Carbon 2011碳科学技术大会圆满结束。大会学术讨论内容主要为石墨烯，纳米碳，碳储能和转换，多孔碳吸附，碳纤维和复合材料，前躯体、碳化和石墨化，计算和模拟，生物碳和安全，新的实验技术和表征，块状碳及工业应用等，涵盖了目前全世界碳科学领域最前沿、最热门、最普遍的研究方向。
Approaches－to－Identifying－Genes－for－Salinity－Tolerance－and－the－Importance..
Soil salinity reduces the ability of plants to take up water, and this quickly causes reductions in the rate of cell expansion in growing tissues.
Complete－Mouse－Necropsy
EuthanasiaEuthanasia and mouse necropsies require prior IACUC approval. The mode of euthanasia should be chosen which minimizes pain or distress to th
补体结合反应(complement－fixation－test)技术概况
可溶性抗原，如蛋白质、多糖、类脂质和病毒等，与相应抗体结合后，抗原抗体复合物可以结合补体，但这一反应肉眼不能察觉，如再加入红细胞和溶血素，即可根据是否出现溶血反应来判定反应系统中是否存在相应的抗原或抗体。这个反应就是补体结合反应。 补体结合反应是一种古老的血清学技术，Bordet和Gengou在19
Immunofluorescent－Staining－of－Drosophila－Larval－Brain－Tissue
实验概要The Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem cells)
Double－immunofluore...
实验概要We provide a protocol for immunofluoresent double staining incubating the antibodies together.In order to be able to examine the co-distributi
艾捷博雅磁性固相萃取技术亮相ASMS－2024美国质谱学会年度会议暨展览会
引言The conventional column and plate solid-phase extraction (SPE) format encounter various unresolved issues, such as inconsistent flow rates, column c
RNA点线杂交
RNA点线杂交（Dot and Slot blotting）· RNA dot blot and slot blot (Beverly Faulkner-Jones)This methods allows the rapid analysis of numerous small sa
Exposing－gels－and－plates－containing－radioactive－samples－to－Xray－film
Although most people use the PhosphorImager for western blots, kinase assays and methionine-labeled samples, X-ray film remains the best way to expose
Thommes博士将在Carbon－2011年会上作分会报告
世界碳科学年度大会(Carbon 2011)将于2011年7月24日-29日在上海华东理工大学召开，美国康塔仪器公司作为金牌赞助商参加此次会议。康塔公司首席科学家——Dr. Matthias Thommes，将作大会分会报告。 报告题目：Characterization of ca
DNA－isolation－extraction
CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolation
超疏水材料的接触角测试：荷叶（lotus－leaf）
超疏水材料的接触角测试过程，示例中采用了荷叶作为测试的样品。超疏水材料的接触角测试非常特殊，由于此时微小的重力均会对接触角产生明显影响，因而，此时只有Young-Laplace方程拟合法才能完成测试。通常的算法，如圆拟合、椭圆拟合均不符合要求，更谈不上落后的量高、量角等方法。而在硬件方面的特殊要求是
PEGMediated－Protoplast－Transformation－with－Naked－DNA
Direct introduction of DNA into plant protoplasts facilitates a rapid analysis of transient gene expression, as well as the generation of stably t
Immunocytochemistry...
实验概要The method provides a guideline procedure for staining of cell cultures using immunofluoresence.实验步骤1. General procedure 1) Coat coverslips wit
TEM－Specimen－Preparation：Preparative－Techniques－for－the－TEM
For routine transmission electron microscopy (TEM), it is generally accepted that specimens should be thin, dry and contain molecules which diffract e
SBA5型CO2气体分析仪参与作物对大气变化响应的相关研究1
——探究大气成分变化对美国中西部地区作物农艺性状和产量的影响，以寻找应对这一挑战的解决方案来自美国农业部农业研究局（USDA-ARS）和伊利诺伊大学厄巴纳-香槟分校创造了SoyFACE（Soybean Free Air Concentration Enrichment）技术，用于研究在实际野外生产条
FISH－protocols－for－Drosophila1
.1 RNA Probe Preparation (see Note 1)1. 1.5 mL microcentrifuge tubes or standard 96-well V-bottom microplates.2. RNAse free water.3. T7, T3 or S
Immunofluorescent－Staining－of－Mouse－and－Rat－Leukocytes
I. ProcedureHarvest cells from tissue, preparing a single cell suspension. Red blood cells may be removed by lysis or density gradient: Red blood cell
Intracellular－Immunofluorescent－Staining－for－Flow－Cytometry2
Table 2: Human Cytokines: Intracellular Staining Quick GuideHuman Cytokines: Intracellular Staining Quick GuideHuman CytokineCell SourceActivationIncu
Immunofluorescent－Staining－of－Drosophila－Larval－Brain－Tissue
INTRODUCTIONThe Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem
Immunofluorescence－Labeling－of－Cells
实验概要Antibodies are an important tool for demonstrating both the presence and the subcellular localization of an antigen. Cell staining is a very ver
Chemotaxis－Assay
PurposeThe purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cel
Chemotaxis－Assay趋化性实验
Springer Lab，The CBR Institute for Biomedical Research, Inc. Department of Pathology Harvard Medical Schoolhttp://cbr.med.harvard.edu/investigators/sp
Isolation－of－Actin－and－Myosin－Filaments
LEVEL IIIMaterialsRelaxing Solution0.1 M KCl0.001 M MgCl5 mM ATP0.016 M NaHPONaHPOAdjust pH to 7.30.05 M Sodium phosphate buffer, pH 7.00.001 M EDTA (
Immunofluorescence－Microscopy－Protocol
实验概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which i
Immunofluorescence－Microscopy－Protocol
实验概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which i
Application－Note:－Qdot®－Nanocrystal－Conjugates－in－Flow－Cytometry
实验概要Researchers today are trying to maximize the information that they get out of flow cytometry experiments by looking at more parameters in a sing