CellSortingByFACS
Currently, the Moflo instrument (Dakocytomation) is used to sort cells at the AECOM FACS facility.Sorting is performed by the person in charge at the facility.You need to book an appointment in advance. (at calendar.yahoo.com, use aecom_moflow / facs**** to log in).Stain cells for FACS as usual but under sterile conditions (unless you don't need to grow the sorted cells). Note that certain staining......阅读全文
Cell－Sorting－By－FACS
Currently, the Moflo instrument (Dakocytomation) is used to sort cells at the AECOM FACS facility.Sorting is performed by the person in charge at the
Detection－Of－Cell－Viability－And/Or－Apoptosis－By－Flow－Cytometry－(FACS)
Viable cells are cells that when allowed to continue beyond the timepoint of examination will stay alive. Besides live and healthy cells, cells in ear
Flow－Sorting－Fibroblasts－with－GFP－and－P.I.
ProtocolWash cells with PBS and trypsinize to a single cell suspension.Count an aliquot on a hemocytometer. Meanwhile centrifuge the cells (e.g. 1000
ex－vivo－expanded－endothelial－progenitor－cells
Cell Culture. 1. Total hPBMCs were isolated from blood of human volunteers by density gradient centrifugation. 2. Cells were plated on culture dishes
FACS－Analysis－of－ES－Cells
Isolate cells and dissociate to single cell suspension (can use Gibco Cell Dissociation Buffer, Accutase or Trypsin)Wash with 10% FBS/DMEM:F12For surf
FACS－Procedures－for－Apoptosis－Detection
Materials:Hoechst 33258 (Sigma B-2883).stock: 10 mg/ml in dH20 (40)working dilution: 500µg/ml (50µl stock + 950µl PBS).7-Amino-actinomycin (Sigma A-94
Comparison－of－Enzymatic－and－NonEnzymatic－Means3
MTT Assay on Reattached CellsAs seen in Fig. 2 , the proportion of viable MSC that re-attached was significantly higher (p = 0.0004) upon dissociati
Purification－of－Lin,－ckit+,－Sca1+－bone－marrow－cells－for－Culture
MATERIALSMedia:Heat inactivated FBS (56°C x 30 min)PBS + 2% heat-inactivated FBSIMDM + 20% heat-inactivated FBSViral transduction: Iscove's + 20%
Enrichment－of－PBMCs－with－monocytes－(The－Science－Advisory－Board)
DescriptionThis protocol is used in our lab to reduce the costs of the cell sorting with MACS reagents. The cell suspension obtained after this protoc
FACS－Analysis－Using－Peripheral－Blood－Cells
FACS Analysis Using Peripheral Blood CellsCollect blood (75 microliters) into 1ml PBS containing 5 microM EDTA (10 microliters of 0.5 M stock) and mix
Protocol－for－intracytoplasmic－staining－of－cytokines－for－FACS－analysis
DescriptionProtocol for intracytoplasmic staining of cytokines for FACS analysis Procedure1) Prepare spleen, lymph node or T cell clone cells as singl
青岛能源所单细胞功能分选技术取得新进展
现有基于荧光染色的活体单细胞分选技术(Fluorescence-Activated Cell Sorting; FACS)一般需要外加荧光标记，在单位时间只能获得个别分子所代表的表型，且通常难以直接进行“原位”观测。而基于拉曼显微光谱技术的单细胞分选方法无需外加标记，可无损获得整个单细胞的化
流式细胞分选的那些事
将感兴趣的目的细胞分离纯化出来，一直是细胞生物学中的一个重要研究手段。无论是体外刺激研究细胞因子的表达谱，还是细胞共培养检测细胞功能，均对细胞的纯度具有很高的要求。目前分离纯化细胞的手段主要有两种：MACS（magnetic-activated cell sorting），原理就是用结合了磁
流式细胞检测
技术原理 流式细胞技术（flow cytometry，FCM）是利用流式细胞仪进行的一种单细胞定量分析和分选技术。是一种在液体系统中，测定单个细胞或细胞器的生物、物理和化学特性，并根据这些特性将所需要的细胞或细胞器进行定量分析和分选的技术。它是一种可以检测细胞或者生物学颗粒的多种特性的技
Use－of－the－B.D.－FACS－or－Calibur－Flow－Cytometers
Reservations:Schedule time on a cytometer (FACS or Calibur) by filling in the calendar at the flow lab. Don't grossly overbook since we have to pa
Fluidigm公司微液流芯片在单细胞研究中的应用（一）
Nature杂志在2009年5月7日的主页文章中大篇幅地介绍了美国Fluidigm公司的微液流芯片在单细胞表达中的应用。A closer look at the single cellMegan ScudellariNature Reports Stem CellsPublished online:
体外荧光法检测核内体早期动力学
A fluorescence-based in vitro assay for investigating early endosome dynamicsSina V Barysch1,2, Reinhard Jahn1 & Silvio O Rizzoli2ABSTRACTEarly endoso
用流式法（FACS)分离间质SP细胞实验
利用Hoechst33342外流性质分选角膜间质干细胞 用ABCG2表达特性免疫分选角膜间质干细胞 实验方法原理
Use－of－the－B.D.－FACS－or－Calibur－Flow－Cytometers2
Gating:Gating will allow you to view cells of interest by any combination of criteria that you choose. Gating does not change the intensity value assi
用流式法（FACS)分离间质SP细胞实验
实验方法原理 实验材料 2〜4代的间质细胞试剂、试剂盒 HBSS 2FBHoechst33342染料 lmg ml水溶碘化丙啶（PI) 2mg ml水溶维拉帕米 500μg ml水溶DMEM 2FB胰蛋白酶 TrypLE:TrypLEExpress或0.25%胰蛋白酶 溶于CMF-SalineG仪器
用流式法（FACS)分离间质SP细胞实验
利用Hoechst33342外流性质分选角膜间质干细胞用ABCG2表达特性免疫分选角膜间质干细胞实验方法原理实验材料2〜4代的间质细胞 试剂、试剂盒HBSS 2FB
发现有望根除侵袭性乳腺癌新方法
常规化疗通常不能够根除侵袭性乳腺癌，这是因为这种癌症在很早就发生肿瘤转移。三阴性乳腺癌（triple-negative breast cancer, TNBC）是一种极具侵袭性的乳腺癌亚型，当前没有靶向治疗方法。最近，人们已发现癌基因MYC在TNBC中增加表达，这就为开发新的靶向治疗策略来选择性
用流式法（FACS)分离间质SP细胞实验1
利用Hoechst33342外流性质分选角膜间质干细胞实验材料2〜4代的间质细胞试剂、试剂盒HBSS 2FBHoechst33342染料 lmg ml水溶碘化丙啶（PI) 2mg ml水溶维拉帕米 500μg ml水溶DMEM 2FB胰蛋白酶 TrypLE:TrypLEExpress或0.25%胰蛋
用流式法（FACS)分离间质SP细胞实验2
实验材料2〜4代的间质细胞试剂、试剂盒PBSA BSA：PBSA+0.5%BSAPBSA 2FB:含2%FBS的PBSA封闭缓冲液抗体：MAB4155F克隆5D3抗ABCG2-FITC或同型抗体-FITC仪器、耗材具有488 nm氩激光器和525 20带通滤波器的高速细胞分选仪实验步骤(a)胰蛋白酶
细胞分选，不仅仅是FACS和MACS
细胞分选（cell sorting）是根据细胞的某种特性，从混合细胞样本中挑选出特定细胞。一提到细胞分选，大家的脑海中可能马上涌现出MACS或FACS。其实，新型的分选技术也在不断涌现，并推动这项技术应用在更广泛的领域。细胞分选火热增长“细胞分选技术的应用呈爆炸式增长，在过去五年中大约翻了一番，”A
特定细胞类型标记的细胞核分离
从大量的植物或动物组织中分离出特异性的细胞类型是费力和棘手的。为了研究表观遗传学上的改变和在不同细胞系中不同表达的基因，如癌细胞与正常细胞，或者拟南芥中两种类型的表皮细胞(epidermal cell)，人们通常必须采用激光捕获显微切割(laser capture microdissection,
青岛能源所基于pDEPRADS推出首台高通量流式拉曼分选仪
单个细胞是生命活动的基本单元，也是生物进化的基本单位。因此单细胞技术正在推动生命起源、细胞功能异质性机制、生命暗物质挖掘与利用等领域的一系列重大突破。单细胞拉曼光谱（SCRS）能非标记、非侵入性、无损、全景式地揭示细胞代谢状态，因此基于拉曼光谱的单细胞分选（Raman-Activated Cel
流式细胞仪做血细胞
Collect blood (75 microliters) into 1ml PBS containing 5 microM EDTA (10 microliters of 0.5 M stock) and mix immediately to prevent clotting. Keep tub
流式细胞仪做血细胞
Collect blood (75 microliters) into 1ml PBS containing 5 microM EDTA (10 microliters of 0.5 M stock) and mix immediately to prevent clotting. Keep tub
The－role－of－FYVEfinger－proteins－in－vesicle－transport
Eukaryotic cells take up constituents of the extra-cellular environment and regulate the cell-surface level of membrane proteins via a recycling syste