Tamoxifenadministrationtomice
One of the uses of tamoxifen in the lab is the activation of enzymes deactivated via an estrogen-binding domain, like Cre-ERT (Cre-LBD). Tamoxifen is an estrogen analogue that binds with higher affinity than estrogen to altered estrogen binding domains. There are several ways to administer tamoxifen to mice each with advantages and disadvantages. If precise dosage is not paramount, administration via f......阅读全文
Tamoxifen－administration－to－mice
One of the uses of tamoxifen in the lab is the activation of enzymes deactivated via an estrogen-binding domain, like Cre-ERT (Cre-LBD). Tamoxifen is
Blood－collection－and－administration－of－fluids－and－drugs－(mouse)
Administration of Fluids and Drugs (Overview):Gastro-Intestinal Tract:Oral or per os (po)- - through the mouthGavage - - into the stomach via a feedin
mice－islet－isolation
1. Islets of Langerhans were isolated from 5- to 7-week-old nonobese diabetic (NOD) mice. 2. Which involves cannulation of the common bile duct and
Blood－collection－and－administration－of－fluids－and－drugs－(mouse)2
Intraperitoneal:Equipment: Syringe and 23 to 27 g, 1/2 to 1-inch needle, preferably with a short bevel.Volume: The volume injected IP into an adult mo
Osteoblast－Harvest(Adult－Mice)
MaterialInstrumentsSolutions1 Curve Scissor50 ml tube 70% Etoh1 Straight ScissorEtoh Spray bottle1 Bone Scraper1 bottle of PBS2 Forceps (1 medium, 1
Harvesting－Hematopoietic－Cells－from－Mice
Materials4 mice from each genotype4 Ly5 miceBuckets with wet ice 3xBucket with dry ice 1xDewar flask with liquid nitrogen100 mL beakers with 95% ethan
Isolation－of－kidney－glomeruli－from－mice
Isolation of mice glomeruli1. Mice were anesthetized by an intraperitoneal injection of Avertin (2,2,2-tribromoethyl and tertiary amyl alcohol; 17
Change－in－Hemoglobin－Levels－due－to－Anesthesia－in－Mice3
The mean hemoglobin in group 1 was 9.9 mmol/L prior to the anesthesia and decreased to 8.7 mmol/L 10 min after administration of the anesthesia (p
Transplantation－of－Pancreatic－Islets－Intothe－Kidney－Capsule－ofDiabetic－Mice
Preparation of Islets for Transplant (Tx)Under an inverted microscope, hand-pick islets using a P200 pipetman and straight pipet tip from the cultured
Change－in－Hemoglobin－Levels－due－to－Anesthesia－in－Mice2
Table 1 The design of the cross-over studyBlood sample Number (days)Group A (n = 6)Group B (n = 6)1 (0)+ anesthesia− anesthesia2 (7)3 (14)4 (21)5 (28)
Change－in－Hemoglobin－Levels－due－to－Anesthesia－in－Mice1
An Important Confounder in Studies on Hematopoietic DrugsAnalgesic and anesthetic drugs may have an impact on the results achieved from animal experim
Guidelines－for－Retroorbital－Bleeding－in－Laboratory－Rats－and－Mice
Materials Needed:Micro pipettes (l00 µl) or Pasteur pipettes drawn to fine tip2X2 gauze squaresNon-sterile glovesEppendorf or other tubes to hold samp
Protocols－for－the－preparation－of－tumour－cells－for－s.c.－injections－in－mice
For the establishment of solid tumour in nude mice, tumour cells are treated in a sterile environment under a cell culturing hood. Disposable sterile
HLA－Typing－for－A2.1－Transgenic－Mice,－protocol1
DNA Digestion and Extraction from Mouse Tailv Cut about 5mm of mouse tail, place in a 1.6mL eppendorf tube on ice (if very cold, the tail is not going
HLA－Typing－for－A2.1－Transgenic－Mice,－protocol2
2) Nested PCR: amplification product is 715 bp.Primer AL#22: CAC TCC ATG AGG TAT TTC TT Primer AL#Q: CTC TCT GCT GCT CCG CCADNA preparation: make a 1:
Protocols－for－the－preparation－of－tumour－cells－for－s.c.－injections－in－mice
For the establishment of solid tumour in nude mice, tumour cells are treated in a sterile environment under a cell culturing hood. Disposable sterile
In－Vivo－Luciferin－Imaging－Procedure
Mice are injected by an intraperitoneal route with a Luciferin solution (15 mg/mL or 30 mg/kg, in PBS, dose of 150 mg/kg) that is allowed to distribut
无创血压计应用论文：动物用血压计（三）
TABLE IPrimer sequences for mRNA analyses Sequences are listed 5' to 3'.Quantitative Real-time PCR by SYBR Green Detection Method-The mRNA l
无创血压计应用论文：动物用血压计（四）
Ang II-stimulated Cardiac Dysfunction in ARKO Male Mice-We also evaluated the cardiac performance of these mice using echocardiography. Although l
FOSB－gene－expression－and－drug－abuse
Drug addiction is associated with long-term behavioral changes, suggesting a long-lived transcriptional regulator that responds to chronic drug exposu
TLR9人源化小鼠（BhTLR9－mice）的应用
我们百奥赛图也为大家送来了“年货” -TLR9人源化小鼠（B-hTLR9 mice），一起来看看吧~ B-hTLR9小鼠基本信息 TLR9基因功能 TLR9（Toll-like receptor 9） 是由TLR9基因编码的一种蛋白质，也称为CD289（分化抗原决定簇289
Invivo－evaluation－ofdrug－lead－candidates－by－intravenous－continuous－infusion
In vivo evaluation of drug lead candidates by intravenous continuous infusionSang Ho LeeWesley ShoopBruce MichaelThomas FelcettoSheo SinghJun Wang , j
营养干预糖尿病研究取得系列进展
热量限制（calorie restriction, CR）是目前国际上公认能够改善代谢并延长寿命的最佳手段，但持续的热量限制在实际应用中有一定难度，因此近年来有人提出了间歇性热量限制的概念，即只限制热量一段时间，然后恢复正常进食。中国科学院上海营养与健康研究院陈雁课题组对间歇性热量限制是否能有效
癌症治疗：光照的黑暗一面
随着生活水平的改变，乳腺癌已经成为摧残部分女性身体的恶魔，是女性最常见的恶性肿瘤之一。据资料统计，乳腺癌发病率大约占各种恶性肿瘤的7-10%。它的发病与遗传有关，以40-60岁之间、绝经期前后的妇女发病率较高，仅约1-2%的乳腺患者是男性。这一通常发生在乳腺上皮组织的恶性肿瘤严重影响了女性的身心
BhC5AR1－mice－–－补体系统新靶点药物研究模型
百奥赛图制备了一系列免疫检查点人源化小鼠，并利用人源抗体进行了有效验证，为免疫检查点抗体药物体内药效评价提供了有效模型和有力工具！今天，小编着重为大家介绍一个行之有效的免疫研究模型 -B-hC5AR1 mice。 背景介绍 在肿瘤免疫中消除免疫抑制细胞，如骨髓细胞和中性粒细胞
BhC5AR1－mice－–－补体系统新靶点药物研究模型
百奥赛图制备了一系列免疫检查点人源化小鼠，并利用人源抗体进行了有效验证，为免疫检查点抗体药物体内药效评价提供了有效模型和有力工具！今天，小编着重为大家介绍一个行之有效的免疫研究模型 - B-hC5AR1 mice。 背景介绍在肿瘤免疫中消除免疫抑制细胞，如骨髓细胞和中性粒细胞，以允许效应细胞的再激
复旦大学Nature子刊发表癌症研究新成果
来自复旦大学、上海中医药大学等机构的研究人员证实，COPS5扩增和过表达通过降解NcoR赋予了Erα阳性乳腺癌对它莫西芬（tamoxifen）的耐药性。这一研究发现发布在7月4日的《自然通讯》（Nature Communications）杂志上。 复旦大学附属肿瘤医院检验科的郭林（Lin Gu
vaxart－疫苗
Vaxart 组建于2004年，公司位于南圣弗朗西斯科，是一家致力于口服重组疫苗开发的私人公司。 1发展历史 2技术平台 疫苗抗原传递技术平台：载体-佐剂结合物可与多数的重组抗原结合后用于口服方式接种； 新开发的非复制性的腺病毒5型载体(Ad5)和经Toll样受体(TLR)起作用的免
CMFDA－Labeling－of－Platelet
OUTLINECMFDA (5-chloromethylfluorescein diacetate) is a lipophilic tracer that has an enormous advantage over ordinary tracers (e.g. FITC) because it
Erythropoietin－mediated－neuroprotection－through－NFkB
Erythropoietin (Epo) is most commonly known as the cytokine secreted by the kidneys that stimulates red blood cell production and is used as a drug fo