ControlofskeletalmyogenesisbyHDAC&calcium／calmodulindependentkinase
The differentiation of muscle cells is transcriptionally regulated, in part by the myocyte enhancer factor-2, MEF2. During myogenesis MEF2 binds to MyoD and other basic helix-loop-helix factors to activate transcription of genes involved in muscle cell differentiation. Transcriptional activation by MEF2 is blocked by interaction with HDAC5 and other histone deacetylases. In undifferentiated myoblasts, HDAC5 is presen......阅读全文
Control－of－skeletal－myogenesis－by－HDAC－calcium/calmodulindependent－kinase
The differentiation of muscle cells is transcriptionally regulated, in part by the myocyte enhancer factor-2, MEF2. During myogenesis MEF2 binds to My
Signal－Dependent－Regulation－of－Myogenesis－by－Corepressor－MITR
The differentiation of muscle cells is regulated by many factors, including the MyoD/MEF2 family of transcription factors. The MyoD/MEF2 dimer binds t
Role－of－MEF2D－in－Tcell－Apoptosis
Mef2 (Myocyte enhancer factor 2) transcription factors play a role in T-Cell Calcium Induced Apoptosis. Several factors regulate Mef2 transcription fa
Pest－Control
We use Dimilin G (4% diflubenzuron) from Maag AG to fight the small dipteran black flies (Trauermücken). It is a granular substance and sprinkled
gSecretase－mediated－ErbB4－Signaling－Pathway
The HER4/erbB4 receptor tyrosine kinase is a member of the EGF1 receptor family. HER4 is a receptor for the neuregulins (NRGs), a family of growth and
Protein－Kinase－A－at－the－Centrosome
Protein kinase A regulatory subunit RIIalpha (PKA-RIIa) is tightly bound to centrosomal structures during interphase through interaction with the A-ki
HISTONE－KINASE－ASSAY
PROTOCOLTo 1.5 mL eppendorf tubes add:200 µg of protein extract (see Western blot protocol for protein sample preps)q.s. to 300 µL with RIPA (with pro
Preparation－of－Conventional－Actin－from－Skeletal－Muscle
Modified from Spudich & Watt, 1971, JBC 246:4866.1. Mix 20 ml buffer G with each gm of muscle acetone powder. Extract with stirring on ice for 30 min.
Angiotensin－Protein－Kinase－Assay
James Hardwick's angiotensin assay protocolThis specific procedure was developed to assay the activity of the Lck kinase expressed from a retrovir
SAPK/Jun－kinase－assays
Preparation of cell lysate:1. For cells in suspension, grow in DMEM with 10% FCS and then the day before you do the experiment spin the cells down (1
Cell－Cycle:－G1/S－Check－Point
The G1/S cell cycle checkpoint controls the passage of eukaryotic cells from the first 'gap' phase (G1) into the DNA synthesis phase (S). Two
Reversal－of－Insulin－Resistance－by－Leptin
The insulin resistance of type II diabetes appears to be caused in part by the presence of high levels of lipids in cells such as skeletal muscle wher
SREBP－control－of－lipid－synthesis
Sterol-regulatory element binding proteins (SREBPs) play a key role in transcriptional regulation of cholesterol metabolism in response to cholesterol
Regulation－of－PGC1a
Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1a) is a tissue-specific coactivator that enhances the activity of many nucl
Skeletal－muscle－hypertrophy－is－regulated－via－AKT/mTOR－pathway
Skeletal muscle atrophies with disuse while with increased use and increased load skeletal muscle exhibits hypertrophy, with an increase in the size o
Human－Cytomegalovirus－and－Map－Kinase－Pathways
To replicate in the host cell, viruses commandeer cellular signaling pathways. Cytomegalovirus (CMV) is a DNA virus with that is widespread in the pop
Sprouty－regulation－of－tyrosine－kinase－signals
Four different members of the Sprouty protein family block the cellular proliferation and differentiation induced by several different growth factors,
Map－Kinase－Inactivation－of－SMRT－Corepressor
Corepressors are coregulators that interact with transcriptional silencers in a variety of pathways such as cell proliferation, differentiation and ap
International－Process－Analytics－and－Control－Congress
International Process Analysis and Control Congress, China 2012IPAC 2012 Call for paper and Introduction The International Process Analysis & Control
筛分仪－AS－200－control特点
德国RETSCH（莱驰）的AS200系列分析筛分仪广泛应用于科研与开发，原材料、半成品和成品的质量控制及生产监控等领域。其可控电磁驱动能为每一产品提供最优的配置。尖锐样品也能在短时间内过筛。所有AS 200 系列机型均采用电磁驱动动力。这是RETSCH 的一项ZL技术（EP 0642844）。这
筛分仪－AS－400－control特点
AS400control分析筛分仪广泛应用于科研与开发，原材料、半成品和成品的质量控制及生产监控等领域。该机型应用于干筛，其所使用的分析筛的直径最大可达到400 毫米。此机型所产生的均一的、水平转动式的筛分运动使筛分物能够得以精确地分离。对于十分注重精确度和操作舒适性的用户，AS400系列的全
Industry－Update－－Uptime－and－Control－in－Petrochemicals
The requirements for petrochemicals and related products are continuously updated and adapted to changes in the industry. The challenge for prof
筛分仪－AS－300－control特点
德国RETSCH（莱驰）的AS300control分析筛分仪广泛应用于科研与开发，原材料、半成品和成品的质量控制及生产监控等领域。其可控电磁驱动能为每一产品提供最优的配置。尖锐样品也能在短时间内过筛。AS 300 control 机型是专门为直径为305毫米（12英寸）的分析筛而设计的。与直径为20
Control－of－Gene－Expression－by－Vitamin－D－Receptor
The vitamin D receptor, VDR is the mediator of all genomic actions of vitamin D3 and its analogs. It belongs to a family of ligand induced transcripti
Proepithelin－Conversion－to－Epithelin－and－Wound－Repair－Control
This diagram illustrates the opposing effects that Proepithelin (PEPI) and the elastase digest fragments, the epithelins (EPIa-g), have on epithelial
Activation－of－cAMPdependent－protein－kinase,－PKA
G-protein coupled receptors (GPCRs) are one of the largest gene families of signaling proteins. Residing in the plasma membrane with seven transmembra
BLOCKiT™－Fluorescent－Oligo－as－RNAi－Transfection－Control
实验概要Intended UseDynabeads® Streptavidin are ideal for numerous applications, including purification of proteins, nucleic acids purification, protein
筛分仪Sieve－Shaker－AS－450－control特点
振荡筛分仪AS450适用于研发过程中原材料、半成品和成品的质量控制，同样适用于生产的监控。可控电磁驱动为每种产品提供了最优的方案。尖锐样品也能在短时间内过筛。振动筛分仪As450 control采用RETSCH连续能源转换技术（CET Technology），比常规筛分仪提供更高的能量，即使再最
Regulation－of－MAP－Kinase－Pathways－Through－Dual－Specificity－Phosphatases
Mitogen-activated protein (MAP) kinases are important players in signal transduction pathways activated by a range of stimuli and mediate a number of
Identification－of－a－Mutant－Kinase/ATP－Analog－Pair2
Cellular Transfection and Immunoprecipitation Before proceeding with the experiments outlined below, all kinase pocket mutants should be characterize